Guinea pig tumor cells

A clone of guinea pig tumor cells, 104C1 (the cells were donated by Dr. Charles H. Evans, National Cancer Institute, Bethesda, MD), was maintained in our laboratory (2 ) on RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco). Cultures were grown in Falcon T-flasks (75 cm2) containing 15... 

Figure 4. Phase contrast micrographs of 104CI guinea pig tumor cells grown in culture (X100)...

The incidence of ovarian tumors in mice, guinea pigs, and rabbits increased after 3 years of chronic irradiation at doses as low as 1.1 mGy daily (Lorenz et al. 1954). Unlike other tumors, the induction of ovarian tumors depended on a minimum total dose and seemed to be independent of a daily dose (Lorenz et al. 1954). Radiation-induced neoplastic transformation of hamster cells may be associated initially with changes in expression of the genes modifying cytoskeletal elements (Woloschak et al. 1990b). 

High doses of dioxane by oral administration produced malignant tumors of the nasal cavity and liver in rats, and mmors of the liver and gallbladder in guinea pigs." Rats administered either 0.5% or 1.0% (vol/vol) in the drinking water had squamous cell carcinomas of the nasal turbinates hepatocellular adenomas were seen in the dosed females." In another study, inhalation of 111 ppm, 7 hours/day, 5 days/week for 2 years did not result in any increased tumor incidence in rats. ... 

When crude endotoxin from the heptose-less mutant of Salmonella typhimurium is combined with trehalose dimycolate from mycobacteria in oil droplets and injected directly into established tumors (line 10 hepatocellular carcinoma) in syngeneic guinea pigs, rapid regression of the tumors occurs and over 90% of the animals are cured. The three required components for activity in this tumor model are (a) the endotoxin (b) the mycobacterial adjuvant, trehalose dimycolate and (c) a compound satisfying the minimal structural requirement (muramyl dipeptide) for adjuvant activity by bacterial cell wall materials. The mycobacterial cell wall skeleton is able to replace the latter two components. 

Carcinogenicity Tg.rasH2 transgenic mouse model Other antigenicity in guinea pigs, mice, and rats tumor cell lines (effects of the product on proliferation) human tumor xenografts in athymic nude mice hemolytic potential studies... 

Figure 1 shows the kinetics of release of radioiodinated protein from antibody-complement-treated tumor cells. Guinea pig serum caused the maximum enhanced release compared to untreated cells of I-labeled protein from anti-Forssman antibody-sensitized cells within 10 min of incubation there was no enhanced release of I-labeled protein compared to controls from anti-line-10 antibody-sensitized cells treated with GPC (Fig. lA). Similarly, HuC caused maximal enhanced release of cell surface protein from cells sensitized with either antibody within 10 min (Fig. IE). No enhanced release of membrane protein was observed from cells treated with antibody alone or complement alone (Fig. 1). The values in Fig. 1 represent 23-40% of the total cell-bound activity associated with [ I]ISA.

T, tumor cells alone TAp or TAtu, cells sensitized with anti-Forssman IgM or antitumor antibody, respectively TCop or TChu. cells treated with guinea pig or human serum (GPC or HuC), respectively TAC, cells sensitized with antibody and treated with complement. 

Antibody-Guinea Pig Serum (GPC)-Mediated Release of I-Labeled Membrane Lipids from Line-10 Tumor Cells"... 

Comparison of the Effects of Guinea Pig and Human Serum (GPC AND HuC) ON THE RELEASE OF I-LaBELED MEMBRANE LiPIDS FROM Line-Tumor Cells ... 

Watson, M.L., Smith, D., Bourne, A.D., Thompson, R-C. and Westwick, J. (1993). Cytokines contribute to airway dysfunction in antigen-challenged guinea pigs inhibition of airway hyperreactivity, pulmonary eosinophil accumulation and tumor necrosis fitctor generation by pretreatment with an interleukin-l receptor antagonist. Am. J. Respir. Cell. Mol. Biol. 8, 365-369. 

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