Genetic engineering protein expression vectors

Li JY, et al. Genetically engineered brain drug delivery vectors cloning, expression and in vivo application of an anti-transferrin receptor single chain antibody-streptavidin fusion gene and protein. Protein Eng 1999 12(9) 787-796. 

Gene Expression Systems. One of the potentials of genetic engineering of microbes is production of large amounts of recombinant proteins (12,13). This is not a trivial task. Each protein is unique and the stability of the protein varies depending on the host. Thus it is not feasible to have a single omnipotent microbial host for the production of all recombinant proteins. Rather, several microbial hosts have to be studied. Expression vectors have to be tailored to the microbe of choice. 

Genetic elements of viruses are ubiquitously used in genetic engineering to achieve supranatural expression in organisms. For viral vectors, the production system involves the use of a functional virus. The expression levels achieved by these systems are typically several percent of soluble protein—consistently higher than achieved by chromosomal integration (Table 2). 

Vaccine production is pursued with the objectives to genetically engineer vaccines with specific gene deletions reducing virulence produce viral and bacterial vectored vaccines and develop subunit vaccines using purified antigens obtained from in vitro expression vectors such as pseudorabies purified protein vaccine and foot-and-mouth disease vaccine. 

What is the purpose of genetic engineering How can human proteins be made by bacteria What is an expression vector ... 

What is the purpose of genetic engineering Once the DNA is successfully cloned, it can be expressed using an expression vector and cell line. This allows for the production of eukaryotic proteins quickly and cheaply in bacterial hosts. Genetically altered organisms, such as mice and corn, have been engineered for both pure and applied scientific purposes, and many more changes are to come. 

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