Antibody single chain

Haidaris, C. G., Malone, J., Sherrill, L. A., Bliss, J. M., Gaspari, A. A., Insel, R. A., et al. (2001) Recombinant human antibody single chain variable fragments reactive with Candida albicans surface antigens. J. Immunol. Meth. 257, 185-202. 

Worn, A. Pltickthun, A., Stability engineering of antibody single-chain fv fragments,./. Mol. Biol. 2001, 305, 989-1010... 

RPPs have been designed to capture proteins from cellular or tissue lysate samples or present in the serum or plasma onto surfaces (12), which are further identified by an affinity reagent such as antibody, single-chain variable fragments, and aptamers, among others. Thus, the signal could be related to specific protein detection and it could be affected by antibody affinity and... 

Monsanto/ Agracetus Monoclonal antibodies, single chain antibodies, Human growth hormone (plastid), collagen 170-177... 

Eldin, P., Pauza, M.E., Hieda, Y, Lin, G., Murtauch, M.P., Pentel, P.R., Pennell, C.A., High level secretion of two antibody single chain Fv fragments by Pichia pastoris. J. Immunol. Methods 1997, 201, 67-75. 

SJ Deng, CR MacKenzie, J Sadowska, et al. Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display. J Biol Chem 269 9533-9538, 1994. 

Okui N, Kobayashi N, Kitamura Y (1998) Production of uninfectious human immunodeficiency virus type 1 containing viral protein R fused to a single-chain antibody against viral integrase. J Virol 72 6960-6964... 

Yamamoto M, Hayashi N, Takehara T, Ueda K, Mita E, Tatsumi T, Sasaki Y, Kasahara A, Hori M (1999) Intracellular single-chain antibody against hepatitis B virus core protein inhibits the replication of hepatitis B virus in cultured cells. Hepatology 30 300-307 Yang 00, Tran AC, Kalams SA, Johnson RP, Roberts MR, Walker BD (1997) Lysis of HIV-1-infected cells and inhibition of viral replication by universal receptor T cells, Proc Natl Acad Sci USA 94 11478-11483... 

The method for the preparation of immunotoxins with SMPB is identical to that used for MBS (above). Since the thioether bonds formed with sulfhydryl-containing molecules are non-cleavable, A-chain isolates or single-chain toxin molecules can not be conjugated with antibodies with retention of cytotoxicity. Only intact A-B toxin molecules may be used with this crosslinker, since the A chain still is capable of being reductively released from the complex. 

K. Grennan, G. Strachan, A.J. Porter, A.J. Killard, and M.R. Smyth, Atrazine analysis using an amperometric immunosensor based on single-chain antibody fragments and regeneration-free multi-calibrant measurement. Anal Chim. Acta 500, 287-298 (2003). 

I. Vikholm and J. Peltonen, Layer formation of a lipid-tagged single-chain antibody and the interaction with antigen. Thin Solid Films 284/285, 924-926 (1996). 

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