Gene fusions

Double barrels have occurred by gene fusion... 

Gene Fusion Systems for Isolation of Cloned Fusion Proteins ... 

AdapLed. from Uhleii, M., and Moles, T., 1990. Gene fusions for purpose of expression An inu oducdon. Methods in Enzymology 185 129-143. 

The PGI and PGII produced from strains transformed with the promoter gene fusion are in all respects tested identical to those enzymes obtained from the wild type strain when grown on pectic substances. For both PGI and PGII the pH optimum is 4.1-4.2 in 50 mM Na-acetate buffer, 30 °C. All further kinetic analyses were performed under these conditions. 

As already mentioned before a complete family of pectin lyases is present in A. niger. With all the genes sequenced and individually expressed, sometimes via promoter gene fusions (see below), the way is open to characterize the full spectrum of activities as outlined in the intro-... 

Optimizing pectinolytic enzyme expression Promoter gene fusions... 

PL activity in extracts from leaf tissue of potatoes transformed with the p35S-PL3 gene fusion. 

Activity of pel uidA gene fusions in strain L37, after leaf inoculation... 

Zakany, J., Tuggle, C. K., Patel, M. D and Nguyen-Huu, C. M. (1988). Spatial regulation of homeobox gene fusions in the embryonic central nervous system. Neuron 1 679-691. 

Rouch, D.A., Parkhill, J., and Brown, N.L., Induction of bacterial mercury- and copper-responsive promoters Functional differences between inducible systems and implications for their use in gene-fusions for in vivo metal biosensors, J Ind Microbiol and Biotechnol, 14 (3), 349-353, 1995. 

Enright, A. J., Iliopoulos, I., Kyrpides, N. C., andOuzounis, C. A. (1999). Protein interaction maps for complete genomes based on gene fusion events. Nature 402, 86-90. 

In this work, we identified a c/.s-acting element associated with the hydA promoter of C. reinhardtii by introducing reporter gene fusions carrying various hydA 5 upstream regions into C. reinhardtii. Our results indicate that a sequence contained within the -128 to -21 region of the hydA promoter is sufficient to confer anaerobic specific gene expression. 

Many of the Philadelphia-positive adult ALL cases (50-75%) have a different type of gene rearrangement. The gene fusion BCR/c-abl takes place after the breakpoints in intron 1 of the BCR gene and the common sequence of the C-abl gene (H2). 

Figure 4. Leukaemic gene fusions involving HATs. (a) The t(8 16) translocation result in the MOZ-CBP fusion protein, while inv(8) gives rise to MOZ-T1F2. (b) Hypothetical model to explain how MOZ-T1F2 and MOZ-CBP fusions result in a similar leukaemia cell phenotype. See text for details, (c) The t(ll 16) and t(l 1 22) result in the MLL-CBP and MLL-p300 gene fusions, respectively...

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