Targeted gene disruption

Cox, D., Condeelis, J., Wessels, D., Soli, D., Kern, H., Knecht, D.A. (1992). Targeted disruption of the ABP-120 gene leads to cells with altered motility. J. Cell Biol. 116, 943-955. 

Chisaka, O., and Capecchi. M. R. (1991). Regionally restricted developmental defects resulting from targeted disruption of the mouse homeobox gene hox-1.5. Nature 350 413-419. 

Caron, K. M., Soo, S. C., Wetsel, W. C., Stocco, D. M., Clark, B. J., and Parker, K. L. 1997. Targeted disruption of the mouse gene encoding steroidogenic acute regulatory protein provides insights into congenital lipoid adrenal hyperplasia. Proc. Natl. Acad. Sci. U.S.A., 94(21) 11540-11545. 

Reduced hepatic uptake and intestinal excretion of organic cations in mice with a targeted disruption of the organic cation transporter 1 (Octl [Slc22al]) gene, Mol. Cell. Biol. 2001, 23, 5471-5477. 

Rohrer, D. K., etal. Targeted disruption of the mouse betal-adrenergic receptor gene developmental and cardiovascular effects. Proc. Natl Acad. Sci. U.S.A. 93 7375-7380,1996. 

Susulic, V. S., Frederich, R. C., Lawitts, J. etal. Targeted disruption of the beta 3-adrenergic receptor gene. /. Biol. Chem. 270 29483-29492,1995. 

A recent study showed significant increases of expression of eNOS in the brains of rats treated with pyrithia-mine [20]. Increased eNOS expression was apparent prior to the onset of neurological symptoms and was restricted to vulnerable medial thalamus and inferior colliculus. Expression of inducible (iNOS) and neuronal (nNOS) isoforms were minimally altered in brain in thiamine deficiency and it has also been shown that targeted disruption of the eNOS (but not the iNOS or nNOS) gene results in reduced extent of neuropathological damage in thalamus of thiamine deficient animals [21] (Fig. 34-5). 

Gunther, U., Benson, J., Benke, D et al. (1995) Benzodiazepine-insensitive mice generated by targeted disruption of the y2-subunit gene of y-aminobutyric acid type A receptors. Proc. Natl. Acad. Sci. USA 92,7749-7753. 

Tretter, V., Hauer, B., Nusser, Z et al. (2001) Targeted disruption of the GABAa receptor 8 subunit gene leads to an up-regulation of y2 subunit-containing receptors in cerebellar granule cells. J. Biol. Chem. 276, 10532-10538. 

Aeai, T., et al., Targeted disruption of pl85/Cul7 gene results in abnormal vascular morphogenesis. Proc Natl Acad Sci USA, 2003, 100(17), 9855-60. 

Borowsky, A., Jirik, F., Krystal, G. and Humphries, R.K., 1998, Targeted disruption of SHIP leads to hemopoietic perturbations, lung pathology and a shortened lifespan. Genes Dev. 12 1610-1620... 

McKercher SR, Torbett BE, Anderson KL, Henkel GW, Vestal DJ, Baribault H, Klemsz M, Feeney AJ, Wu GE, Paige CJ, Maki RA (1996) Targeted disruption of the PU.l gene results in multiple hematopoietic abnormalities. EMBO J 15 5647-5658 Mclean AR, Michie CA (1995) In vivo estimates of division and death rates of human T lymphocytes. Proc Natl Acad Sci USA 92 3707-3711... 

Shull MM, Ormsby I, Kier AB, Pawlowski S, Diebold RJ, Yin M, Allen R, Sidman C, Proetzel G, Calvin D, et al Targeted disruption of the mouse transforming growth factor-pj gene results in multifocal inflammatory disease. Nature 1992 359 693-699. 

Clark JC, Wert SE, Bachurski CJ, et al. Targeted disruption of the surfactant protein B gene disrupts surfactant homeostasis, causing respiratory failure in newborn mice. Proc Natl Acad Sci USA 1995 92(17) 7794 7798. 

TABLE 2. Targeted disruption of genes in the mouse producing osteopetrosis... 

The first gene whose targeted disruption unexpectedly produced osteopetrosis was c-src (Soriano et al, 1991). c-src codes for cytoplasmic tyrosine kinase... 

Dai, X.M., Ryan, G.R., Hapel, A.J., Dominguez, M.G., Russell, R.G., Kapp, S., Sylvestre, V., and Stanley, E.R. (2002) Targeted Disruption of the Mouse Colony Stimulating Factor 1 Receptor Gene Results in Osteopetrosis, Mononuclear Phagoc34e Deficiency, Increased Primitive Progenitor Cell Frequencies, and Reproductive Defects. Blood99, 111 20. 

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