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Fish species identification

CG Sotelo, C Pineiro, JM Gallardo, RI Perez-Martin. Fish species identification in seafood products. Trends Food Sci Technol4 395 -401, 1993. [Pg.164]

SG Armstrong, DN Leach, SG Wyllie. The use of HPLC protein profiles in fish species identification. Food Chem 44 147-155, 1992. [Pg.164]

Rehbein H (1997). Comparison of several types of precast polyacrylamide gels for fish species identification by DNA analysis (single strand conformation polymorphism, and random amplified polymorphic DNA). Arch. Lebensmittelhyg., 48 41-43. [Pg.116]

Rehhein H, Mackie IM, Pryde S, et al. (1999a). Fish species identification in canned tuna hy PCR-SSCP validation by a collaborative study and investigation of intra-species variability of the DNA patterns. Food Chem., 64 263-268. [Pg.117]

In recent decades a number of polymerase chain reaction (PCR)-based methods for fish species identification by DNA analysis have been developed to support food control authorities to achieve this goal. In the following sections these techniques are described... [Pg.209]

The number of fish species with mitochondrial genes sequenced exceeds by far the number of species for which sequences of nuclear genes are known. In April 2008 the database MitoFish (mitofish.ori.u-tokyo.ac.jp) listed 418 DNA sequences of complete mitochondrial genomes and more than 88,000 partial sequences of 10,000 fish species. Comparison of these figures with the total number of fish species (about 29,000) demonstrates the wealth of information available for fish species identification by mitochondrial DNA analysis. [Pg.211]

The use of mitochondrial DNA for fish species identification is made under the assumption that hybridization between species is a rare event. In the case of fish (but not of mussels see below) only the mitochondrial genome of the mother is passed on to the next generation (Brown, 2008). Therefore, it is not possible to differentiate between fish of the maternal species and hybrids by analysis of mitochondrial DNA. Interspecific hybridization has been observed for many freshwater species (Hubbs, 1955), but has also been documented for marine species such as flatfish (Garrett et al., 2007) and redfish (Pampoulie and Danielsdottir, 2008). [Pg.211]

Attempts have been made to accelerate and simplify PCR-based fish species identification by development of microarrays. Up to now these attempts have not been very successful, because the large number of fish, many of them closely related, makes it necessary to perform lengthy tests for the specificity of the oligonucleotide probes. Recentiy, a first prototype has been constmcted to identify 11 commercially important fish species using a fragment of the 16S rRNA gene (Kochzius et al., 2008). [Pg.216]

Species identification of seafood product is important for the implementation of the labeling regulations as set by many countries. These regulations to prevent the substitution of some commercially important fish can be effectively achieved when species-specific data of all fish species are available. Genome and protein techniques are the two valuable methodologies that can be used for fish species identification. These methods can prevent adulteration of toxic puffer species as nontoxic puffer one. Hence, the development of genome and protein techniques for effective identification of puffer species is critically needed. [Pg.209]

At present, PCR-RFLP is the most common DNA-based technique used for fish species identification. Scientists draw the greatest attention to mitochondrial cytochrome b gene. The species-spedfic gene appears to have substantial inter- and intraspedes variation in its original nucleotide sequence, and the... [Pg.210]

Gallardo, J.M., Sotelo, C.G., Pineiro, C., and Perez-Martin, R.l. 1995. Use of capillary zone electrophoresis for fish species identification-differentiation of fiat fish species. J. Agric. Food Chem. 43, 1238-1244. [Pg.224]

Doodley, J. J., Sage, H. D., Clarke, M. A. L., Brown, H. M., and Garrett, S. D., Fish species identification using PCR-RFLP analysis and lab-on-a-chip capillary electrophoresis. Apphcation to detect white fish species in food products and an interlaboratory study, J. Agric. Food Chem., 53, 3348, 2005. [Pg.911]

Meat proteins comprise a water-soluble fraction (containing the muscle pigment myoglobin and enzymes), a salt-soluble fraction composed mainly of contractile proteins, and an insoluble fraction comprising connective tissue proteins and membrane proteins. As reviewed by Dierckx and Huyghebaert [107], HPLC analysis of meat proteins has been successfully applied to evaluate heat-induced changes in the protein prohle, to detect adulterations (addition of protein of lower value, the replacement of meat from high-value species with meat from lower-value species, etc.), and for specie identification in noncooked products (also for fish sample). [Pg.580]

Giri, A., Osako, K., and Ohshima, T. (2010a). Identification and characterization of headspace volatiles of fish miso, a Japanese fish meat based fermented paste, with special emphasis on effect of fish species and meat washing. Food Chem. 120, 621-631. [Pg.101]

C Pineiro, CG Sotelo, I Medina, JM Gallardo, RI Perez-Martin. Reversed-phase HPLC as a method for the identification of gadoid fish species. Z Lebensm Unters Forsch A 204 411-416, 1997. [Pg.164]

SH Ashoor, MJ Knox. Identification of fish species by high-performance liquid chromatography. J Chromatogr 324 199-202, 1985. [Pg.164]

Molecular microbial ecology methods are being developed that fink species identification with activity, typically combining molecular identification methods with isotope labefing. For a comprehensive review of these methods, see Neufeld et al. (2007). One of these approaches combines isotope labeling followed by microautoradiography and hybridization techniques such as FISH (FISH-MAR) and microarrays (isotope arrays). FISH-MAR has been done with H labeled substrates (Cottrell and Kirchman, 2000 Lee et al., 1999 Ouvemey and Fuhrman, 1999), labeled substrates (Lee etal, 1999), and Pi labeled substrates (Lee ei estuarine water with H labeled amino acids, proteins. [Pg.1308]

The medaka (Oryzias latipes) is a teleost native to Japan, Korea, China, and Taiwan23. Similar to the zebrafish, it produces transparent embryos that allow easy observation and manipulation. Medaka is a popular model organism in Japan and the first fish species in which stable transgenesis was established, it provides some complimentary features to the zebrafish such as adaptation to a wide range of temperatures.22 The identification of sex specific markers, secondary sex characters and a sex determination gene have lead to increasing attraction of the medaka as a model for research on sex determination and differentiation23-28 (photo Andre Kunzelmann, UFZ). [Pg.248]

Identification of fish species by their amino acid pattern... [Pg.646]

Digestion of the products with different endonucleases, followed by agarose gel electrophoresis of the digested products, yielded specific restriction patterns that enabled direct visual identification of the species analyzed. This PCR-RFLP methodology allowed not only clear discrimination of different salmon species in raw and smoked products but also of different fish species that may be present in food products. Russell et al. (2000) demonstrated that this method can be used to differentiate between salmon species. The reliability and practicality of the method was also tested by a collaborative study carried out in five European laboratories (Hold et al., 2004). [Pg.99]

The need for high-throughput screening methods of human mutations has stimulated the development of CE-based methods for SSCP analysis. For detection of ssDNA, PCR is carried out with primers labeled at the 5 site with fluorescence dyes. Two different labels may be used for identification of the forward and reverse strands. Advantages of CE-SSCP are speed of electrophoresis (ca. 10 min), high sensitivity, reproducibility, and the possibility of automation (Andersen et al., 2003 Hestekin et al., 2006). In food analysis, CE-SSCP has been used to identify bacteria (see Section 5.4.4) but, to the knowledge of the author, not to species identification of meat, fish, or other food up to now. [Pg.109]

SSCP analysis has been found useful for the authentication of various types of seafood. As shown in Table 5.2, a considerable number of fish species, shrimps, and mollusks could be identified by SSCP of mitochondrial or nuclear genes. As SSCP gives very good results for short DNA segments, species identification of products containing severely degraded DNA (e.g., canned tuna) may be easier to achieve by SSCP than by RFLP (Rehbein et al., 1999). In a study of fish meal authentication, SSCP and RFLP-SSCP allowed differentiation between meals produced from a number of fish species, including two closely related sand eel species (Rehbein, 2002). [Pg.112]

Garcia-Vazquez E, Alvarez P, Fopes P, et al. (2006). PCR-SSCP of the 16S rRNA gene, a simple methodology for species identification of fish eggs and larvae. Scl Mar., 70S2 13-21. [Pg.115]

Rehbein H (2002). Identification of the fish species processed to fish meal. J. Aquat. Food Prod. Technol, 11 45-56. [Pg.116]

Rehbein H (2005). Identification of the fish species of raw or cold-smoked salmon and salmon caviar hy single-strand conformation polymorphism (SSCP) analysis. Eur. Food Res. Technol., 220 625-632. [Pg.117]


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See also in sourсe #XX -- [ Pg.209 , Pg.211 , Pg.212 , Pg.213 , Pg.214 ]




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