Molecular microbial ecology methods

Molecular microbial ecology methods are being developed that fink species identification with activity, typically combining molecular identification methods with isotope labefing. For a comprehensive review of these methods, see Neufeld et al. (2007). One of these approaches combines isotope labeling followed by microautoradiography and hybridization techniques such as FISH (FISH-MAR) and microarrays (isotope arrays). FISH-MAR has been done with H labeled substrates (Cottrell and Kirchman, 2000 Lee et al., 1999 Ouvemey and Fuhrman, 1999), labeled substrates (Lee etal, 1999), and Pi labeled substrates (Lee ei estuarine water with H labeled amino acids, proteins. 

Milde K, Sand W, Wolff W, Bock E (1983) Thiobacilli of the concrete walls of the Hamburg sewer system. J Gen Microbiol 129 1327-1333 Millard SG, Gowers KR, Bungey JH (1995) Galvanostatic pulse techniques a rapid method of assessing corrosion rates of steel in concrete structure. NACE International Corrosion, Houston Miller JDA (1970) Microbial aspects of metallurgy. Elsevier, New York, pp 61-105 Mills AL, Powelson DK (1996) Bacterial interactions with surfaces in soils. In Fletcher M (ed) Bacterial adhesion molecular and ecological diversity. Wiley-Liss, New York, pp 25-57... 

Bockelmann, U., W. Manz, T. R. Neu, and U. Szewzyk. 2000. Characterization of the microbial community of lotic organic aggregates ( river snow ) in the Elbe River of Germany by cultivation and molecular methods. FEMS Microbiology Ecology 33 157—170. 

---