High-throughput screening method

Bevan, C. D., Uoyd, R. S. A high-throughput screening method for the determination of aqueous drug solubility using laser nephelometry in microtiter plates. Anal. Chem. 2000, 72,1781-1787. 

Considering that PAMPA is a high-throughput screening method, the errors are low enough to encourage the use of the method to study mechanistic properties, as our group at pION has done since 1997. 

Hwang, B.-Y. and Kim, B.-G. (2004) High-throughput screening method for the identification of active and enantioselective m-transaminases. Enzyme and Microbial Technology, 34, 429-436. 

W.P. Janzen (Ed.), High Throughput Screening Methods and Protocols, Humana, Totowa, NJ, 2002. 

Typical methods for generation of polymorphs include sublimation, crystallization from different solvents, vapor diffusion, thermal treatment, melt crystallization, and rapid precipitation. High-throughput screening methods have been reported for polymorph screening.50... 

Onofrey, T. and Kazan, G. Performance and Correlation of a 96-Well High throughput Screening Method to Determine Aqueous Drug Solubility, Application Note (Billerica, MA Millipore Corp., 2003). 

Baumann, M., Sturmer, R. and Bornscheuer, U.T. (2001) A high-throughput-screening method for the... 

Chisholm B, Potyrailo R, Shaffer R, Cawse J, Brennan M, Molaison C (2003) Combinatorial chemistry methods for coating development III. Development of a high-throughput screening method for abrasion resistance correlation with conventional methods and the effects of abrasion mechanism. Prog Org Coat 47 112-119... 

DNA assays will certainly play an essential role in future medical research, along with proteomics. This makes high-throughput screening methods necessary. Parallel DNA separation chips coupled with high-sen-sitivity detection such as LIF or mass spectrometry (56) should be able to provide the required structural information in less time than with techniques currently employed (12). 

A product that is widely used, and one that has also grown in importance since the advent of high throughput screening methods, is the AM (acetoxymethyl) ester of the fluoran derivative, Fluo-3 (3.67). This product, Fluo-3AM, does not itself bind Ca but is readily hydrolysed to Fluo-3 by endogenous esterases once the dye is in the cells. 

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