Fine needle aspirates

Fine-Needle Aspiration of Right Breast Mass and Right... 

Biopsy of suspected lymph node(s)—either open lymph node biopsy or core biopsy preferred over fine-needle aspiration. 

Liu J, Farhood A. Immunostaining for thyroid transcription factor-1 on fine-needle aspiration specimens of lung tumors a comparison of direct smears and cell block preparations. Cancer 2004 102 109-114. 

Nizzoli R, Bozzetti C, Crafa P, et al. Immunocytochemical evaluation of Her-2/ nue on fine-needle aspirates from primary breast carcinomas. Diagn. Cytopathol. 2003 28 142-146. 

Kulkarni M, Desai S, Ajit D, et al. Utility of the thromboplastin-plasma cell-block technique for fine-needle aspiration and serous effusions. Diagn. Cytopathol. 2009 37 86-90. 

Nga ME, Lim G-L, Barbro N, et al. Successful retrieval of fine-needle aspiration biopsy material from previously stained smears for immunocytochemistry a novel technique applied to three soft tissue tumors. Mod. Pathol. 2005 18 728-732. 

Musso C, Silva-Santos M, Pereira F. Cotton block method one-step method of cell block preparation after fine needle aspiration. Acta Cytol. 2005 49 22-26. 

Krogerus L, Anderson L. A simple method for the preparation of paraffin-embedded cell blocks from fine needle aspirates, effusions and brushings. Acta Cytol. 1988 32 585-587. 

Beatty BG, Bryant R, Wang W, et al. Her-2/neu detection in fine-needle aspirates of breast cancer fluorescence in situ hybridization and immunocytochemical analysis. Am. J. Clin. Pathol. 2004 122 246-255. 

Masood, S. (1989) Use of monoclonal antibody for assessment of estrogen receptor content in fine-needle aspiration biopsy specimen from patients with breast cancer. Arch. Pathol. Lab. Med. 113, 26-30. 

Rapkiewicz A, Espina V, Zujewski JA et al (1925) The needle in the haystack application of breast fine-needle aspirate samples to quantitative protein microarray technology. Cancer 111 173-184... 

The diagnosis of SCLC is usually made using fiberoptic bronchoscopy. The value of fiberoptic bronchoscopy was established in the 1980s (8,9). Ninety-three percent of SCLC cases are diagnosed by fiberoptic bronchoscopy while fine needle aspiration or mediastinoscopy diagnose the rest. The majority of time, the diagnosis of SCLC is made cytologically. Unfortunately, this limits the amount of tissue available for further studies,... 

Direct laparoscopic visualization of the peritoneum, omentum, and liver surface can spare patients unnecessary surgery. Once a pancreatic mass is considered to be unresectable or if metastasis occurs, then a histologic diagnosis should be established by using direct fine-needle aspiration. 

There is also a trend toward performing minimally invasive biopsy techniques such as needle/core biopsies and fine needle aspirates (FNAs). The amount of material available from such techniques, even if the bulk (non-microdissected) sample were to be used, can be very small. Traditional DNA microarray analysis requires a fairly substantial amount of material for analysis some 5-10 p.g of total RNA is usually required for analysis in the absence of amplification. In order to obtain this quantity of RNA from typical epithelial cells, as many as 5 x 10 or 1 x 10 cells are required. Clearly, this total RNA requirement poses a challenge when studying microdissected samples, but it also presents a challenge when using small, unique clinical samples, such as FNA biopsies. 

Assersohn L, Gangi L, Zhao Y et al. The feasibility of using fine needle aspiration from primary breast cancers for cDNA microarray analyses. Clin Cancer Res 2002 8 794-801. 

Fine-needle aspiration biopsy (FNA) Normal pattern Test not indicated Test not indicated... 

Hughes, D. A, Kempson, M. G., Carter, N. P., and Morris, P. J (1988) Immuno-gold-silver/Romanowsky staining simultaneous immunocytochemical and morphological analysis of fine-needle aspirate biopsies. Transplant. Proc 20, 575,576... 

Another advantage of immunohistochemistry is that tissues of a small size (e.g., biopsies) can be used. This is important because it is better to detect tumors at an early stage, when they are small. The necessity of early detection cannot be overemphasized. Very small tumors and fine-needle aspirates cannot be used for biochemical assays. Although the DCC assay provides quantitative results, it does not take into account the relative amount of the connective tissue in the specimen, the presence of carcinoma in situ lesions, or normal ducts and lobules. These limitations are not encountered when using paraffin sections. In addition, immunohistochemistry allows the use of archival tissues when fresh tissues are not available. This method does not require any special, expensive equipment and can be carried out in any standard laboratory. 

IMMUNOSTAINING OF ESTROGEN AND PROGESTERONE RECEPTORS IN FINE-NEEDLE ASPIRATES OF BREAST... 

Charpin, C., Andrac, L., Habib, M.-C., Vacheret, H., Xerri, L., Devictor, B., Lavaut, M. N., and Toga, M. 1989. Immunodetection in fine-needle aspirates and multiparametric (SAMBA) image analysis. Cancer 63 863-872. 

Leung, S. W., and Bedard, Y. C. 1999. Estrogen and progesterone receptor contents in ThinPrep-processed fine-needle aspirates of breast. Am. J. Pathol. 112 50-58. 

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