Estrogen and Progesterone Receptors in Fine-Needle Aspirates of Breast

IMMUNOSTAINING OF ESTROGEN AND PROGESTERONE RECEPTORS IN FINE-NEEDLE ASPIRATES OF BREAST 

Target Retrieval Solution (Dako S1700) is diluted 1 10 with distilled water and adjusted to pH 6.0. ThinPrep smears are rehydrated in 95% ethanol and distilled water for 5 min each. The smears are placed in a plastic slide container with sufficient retrieval solution. The container is immersed in a sufficient quantity of warm water inside the microwave pressure cooker (Dako, DO300X) and heated in a microwave oven for 20 min at full power until steam is generated. The smears, along with retrieval solution, are allowed to cool for 10 min. The smears are removed and rinsed with warm water, followed 

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