Baby hamster fibroblast cell line

The BHK-21 (baby hamster kidney) cell line consists of adherent fibroblasts, that can also be adapted to suspension culture, and was isolated from five 1-day-old hamsters (McPherson and Stoker, 1962). These cells are commonly used for virus propagation (polio, rabies, and foot-and-mouth disease) for production of veterinary vaccines. 

Fig. 2. Effect of serum concentration on the attachment and spreading of BHK-21 cells onto TCP2 surface. BHK-21 cells were seeded in media containing the indicated concentrations of intact serum (open squares), Fn-depleted serum (triangles). Vn-depleted serum (circles), or serum-free medium alone (the single closed square) and the attachment panel (A) and spreading panel (B) of the cells were determined after 90 min culture on TCP (panel (A, B)) Mean SEM. (Reproduced from J. Biomed. Mater. Res. [Ref. 11 Role of serum vitronection and fibronectin in adhesion of fibroblasts following seeding onto tissue culture polystyrene] through the courtesy of John Wiley Sons, Inc.) BHK-21 Fibroblast cell lines from Baby Hamster Kidney 2 Similar results on Primaria are also presented in [Ref 11]...

The LPC permeabilization technique reproducibly permeabilizes numerous cell types including human and murine T cells, T-cell lines, NK cells, B cells, B-cell lines, fibroblasts, Chinese hamster ovary cells, baby hamster kidney cells, MDCK cells, murine neurons, and COS African green monkey kidney cells, using identical conditions. 

Most of the molecular biology of alphaviruses has been determined using a continuous baby hamster kidney (BHK) cell line or primary chicken embryo fibroblast (CEF) cells infected with either Sindbis (SIN) virus or Semliki Forest (SF) virus. Alphavirus-cell in-... 

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