Human skin fibroblasts

Moy LS, Howe K, Moy RL (1996) Glycolic acid modulation of collagen production in human skin fibroblast cultures in vitro. Dermatol Surg 22(5) 439-441... 

Offord, EA, JC Gautier, O Avanti et al. 2002. Photoprotective potential of lycopene, beta-carotene, vitamin E, vitamin C and camosic acid in UVA-irradiated human skin fibroblasts. Free Radio Biol Med 32(12) 1293-1303. 

Obermuller-Jevic, UC. Schlegel, B. Flaccus, A., and Biesalski, HK. 2001. The effect of beta-carotene on the expression of interleukin-6 and heme oxygenase-1 in UV-irradiated human skin fibroblasts in vitro. FEBS Lett 509 186-190. 

MWNTs were found to be cytotoxic in human skin fibroblasts (HSF42) and human epidermal keratinocytes (HEK) [42-44], whereas SWNTs were toxic in human keratinocyte (HaCaT) cultures [25, 26, 45]. Reduced cell proliferation and oxidative stress were reported also in epithelial (HeLa) cells [45] and murine epidermal cells (JB6 P + ) [46] upon incubation with SWNTs. 

Ding, L. et al. (2005) Molecular characterization of the cytotoxic mechanism of multiwall carbon nanotubes and nano-onions on human skin fibroblast. Nano Letters, 5 (12), 2448-64. 

In contrast to transition metals iron and copper, which are well-known initiators of in vitro and in vivo lipid peroxidation (numerous examples of their prooxidant activities are cited throughout this book), the ability of nontransition metals to catalyze free radical-mediated processes seems to be impossible. Nonetheless, such a possibility is suggested by some authors. For example, it has been suggested that aluminum toxicity in human skin fibroblasts is a consequence of the enhancement of lipid peroxidation [74], In that work MDA formation was inhibited by SOD, catalase, and vitamins E and C. It is possible that in this case aluminum is an indirect prooxidant affecting some stages of free radical formation. 

Reelfs, O., Tyrrell, R. M., and Pourzand, C., Ultraviolet a radiation-induced immediate iron release is a key modulator of the activation of NF-kappaB in human skin fibroblasts, J. Invest. Dermatol. 122, 1440-1447, 2004. 

Figure 7.9. Human skin fibroblasts labeled with dil and illuminated by T1RF at two different angles of incidence, (a) 0=74.3°, d= 105 nm (b) 0 = 67.9°, d=406nm. In this setup, 0C = 67.5°. Notice how more of the cell surface is immersed in the deeper evanescent wave of (b). 

Chen, W.W., Moser, A.B., and Moser, H.W., 1981, Role oflysosomal add ceramidase in the metabohsm of ceramide in human skin fibroblasts. Arch. Biochem. Biophys. 208 444-455. 

A method for quantitative acylcamitine profiling in human skin fibroblasts using unlabelled palmitic acid diagnosis of fatty acid oxidation disorders and differentiation between biochemical phenotypes of MCAD deficiency. 

Vile, G. R, and Tyrrell, R. M. 1995. UVA radiation-induced oxidative damage to lipids and proteins in vitro and in human skin fibroblasts is dependent on iron and singlet oxygen. Free Rad. Biol. Med. 18 721-22. 

Human skin fibroblasts are cultured from skin biopsy samples. The dermis is cut into small pieces (0.5 mm on each side) and placed in a dish containing DMEM, 10% (v/v) FCS, and 1% (v/v) antibiotic-antimycotic solution. When these primary cultures are confluent they are split into novel dishes. Cells between passages three and six are used for experiments. 

Human skin fibroblasts are cultured from skin biopsy samples. The dermis is cut into small pieces (0.5 mm on each side) and placed into a dish in DMEM containing 10% (v/v) FCS and 1% (v/v) antibiotic-antimycotic solution. When these primary cultures are confluent they are split and cells between passage three and six are used for experiments. For the cholesterol efflux assay, cells are grown in 24-well plates to 60-80% confluence and are labeled with [1,2-3H]-cholesterol (1 pCi/well) for 24 h. Cells are then washed with DMEM and incubated for 4 h at 37°C with DMEM containing BSA (0.2%, v/v) and either 0 (negative control) or 5-30 pg/ml apoA-I. The efflux medium is collected and centrifuged to remove cell debris. Cells are solubilized in 0.1 mol/1 NaOH and the radioactivity in the efflux media and in the cell lysates is determined by scintillation counting [11, 30, 75]. 

SHE, Sister chromatid exchange, human lymphocytes in vitro SHE, Sister chromatid exchange, human lymphocytes in vitro SHE, Sister chromatid exchange, human lymphocytes in vitro SHE, Sister chromatid exchange, human lymphocytes in vitro SHE, Sister chromatid exchange, human lymphocytes in vitro SHE, Sister chromatid exchange, human lymphocytes in vitro MIH, Micronucleus test, human blood lymphotyctes in vitro CHF, Chromosome aberrations, human skin fibroblasts in vitro... 

CHF, Chromosome aberrations, human skin fibroblasts in vitro... 

Beckman et al. (28) have studied the electrophoretic separation of the acid phosphatase activity in tissue extracts on starch gel at pH 8. They described four electrophoretic bands A, B, C, and D. Table IV (28) shows the distribution of activity in different organ extracts. The ABD pattern predominated in kidney BD in liver, intestine, heart, and skeletal muscle B in skin and D in pancreas. The C component was present in a large number of placentae but not in other adult organs. All four electrophoretic components were inhibited by d-(- -)-tartrate A contained sialic acid, D had a lower pH optimum and was more heat resistant than A, B, and C. Components C and D showed parallel electrophoretic behavior. In human skin fibroblasts grown in tissue culture, the acid phosphatase was generally high and the most common pattern was BD. Almost every culture showed some activity. The BD... 

Receptor-mediated uptake of LDL by human skin fibroblasts. Specific LDL receptors are located in coated regions of the plasma membrane. LDL binding results in uptake by endocytosis and formation of a coated vesicle. This vesicle fuses with a lysosome containing many hydrolytic enzymes that degrade the lipoprotein, releasing cholesterol. 

Electron micrograph of LDL particles (made electron-dense with covalently bound ferritin) bound to coated regions of a human skin fibroblast (97,000 X ). (From R. G. W. Anderson, M. S. Brown, and J. L. Goldstein, Role of the coated and endocytic vesicle in the uptake of receptor-bound low-density lipoprotein in human fibroblasts. Cell 10 351, 1977. Cell Press.)... 

Glycosaminoglycan synthesis, in culture, using fibroblasts from human skin,97,98 human normal and hypertrophic scar,99 and embryonic chick... 

K. Takigaki, T. Nakamura, A. Kon, S. Timura, and M. Endo, Characterization of /9-D-xyloside-induced glycosaminoglycans and oligosaccharides in cultured human skin fibroblasts. J. Biochem. (Tokyo), 109(1991)514-519. 

A. Schmidtchen, I. Carlstedt, A. Malmstrom, and L. -A. Fransson, Inventory of human skin fibroblasts proteoglycans. Identification of multiple heparan and chondroitin/dermatan sulfate proteoglycans. Biochem. J., 265 (1990) 289-300. 

Groult, V., Hornebeck, W., Ferrari, P., Tixier, J. M., Robert, L., and Jacob, M. P. (1991). Mechanisms of interaction between human skin fibroblasts and elastin Differences between elastin fibers and derived peptides. Cell Biochem. Fund. 9, 171-182. 

Disease Deficient Enzyme Tentative Structure of Glycosphingolipid Accumulating in Human Skin Fibroblasts Abbreviated form of GSL... 

Incorporation of tritium (cpm/mg of protein) into individual moieties of glycosphingolipids of normal human skin fibroblasts in the presence and absence of galactose oxidase... 

Oriente A, Fedarko NS, Pacocha SE, Huang S-K, Lichtenstein LM, Essayan DM. Interleukin-13 modulates collagen homeostasis in human skin and keloid fibroblasts. Journal of Pharmacology and Experimental Therapeutics 2000, 292, 988-994. 

Yamada H, Tajima S, NishikawaT, Murad S, Pinnell SR. Tranilast, a selective inhibitor of collagen synthesis in human skin fibroblasts. Journal of Biochemistry 1994, 116,... 

Isobe, K., Ito, S., Hosaka, H., Iwamura, Y., Kondo, H., Kagawa, Y., and Hayashi, J. I., Nuclear-recessive mutations of factors involved in mitochondrial translation are responsible for age-related respiration deficiency of human skin fibroblasts. J. Biol. Chem. 273, 4601-4606 (1998). 

HUVEC, human skin fibroblasts UV patterning of photoresponsive protein-resistant polymer 2007 [39]... 

Mian, N., Analysis of cell-growth-phase-related variations in hyaluronate synthase activity of isolated plasma-membrane fractions of cultured human skin fibroblasts, Biochem. J., 237, 333, 1986. 

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