Expression purification

W. L., and Rivera, K. E., Facile purification of fibrinogen fragments using a computer-based model with general applicability to the generation of salt gradients, Prot. Expression Purif., 14, 71, 1998. 

Marcy AI, Eiberger LL, Harrison R, Chan HK, Hutchinson NI, Hagmann WK, Cameron PM, Boulton DA, Hermes JD. Human fibroblast stromelysin catalytic domain expression, purification and characterization of a C-terminally truncated form. Biochemistry 1991 30 6476-6483. 

Chen, H. Wang, R. Cerniglia, C. E. Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis. Protein Expression Purif. 2004,34, 302-310. 

Williams MD, Fieno AM, Grant RA, Sherman DH (1996) Protein Expression Purif 7 203... 

Spider Silk and Spider Silk-ELP Fusion Proteins from Plants Expression, Purification and Applications... 

Haining RL, Hunter AP, Veronese ME, et al. Allelic variants of human cytochrome P450 2C9 baculovirus-mediated expression, purification, structural characterization, substrate stereoselectivity, and prochiral selectivity of the wild-type and I359L mutant forms. Arch Biochem Biophys 1996 333(2) 447 t58. 

Pikuleva lA, Bjorkhem 1, Waterman MR. 1997. Expression, purification, and enzymatic properties of recombinant human cytochrome P450c27 CYP27). Arch Biochem Bio-phys 343 123-130. 

Yu AM, Raining RL. 2006. Expression, Purification and characterization of mouse CYP2d22. Drug Metab Dispos 34 1167-1174... 

StoU VS, Manohar AV, Gillon W, Mac Farlane EL, Hynes RC, et al. 1998. A thioredoxin fusion protein of VanH, a d-lactate dehydrogenase from Enterococcus Faecium cloning, expression, purification, kinetic analysis, and crystallization. Protein Sci 7 1147-1155. 

Loudon, R. P., and Benovic, J. L. (1994) Expression, purification, and characterization of the G protein-coupled receptor kinase GRK6. J. Biol. Chem. 269, 22691-22697. 

Huang, R. Y, Boulton, S. J., Vidal, M., Almo, S. C., Bresnick, A. R. and Chance, M. R. (2003). High-throughput expression, purification, and characterization of recombinant Caenorhabditis elegans proteins. Biochem. Biophys. Res. Commun. 307,928-934. 

Balendiran, K., Bonventre, J., Knott, R., Jack, W., Benner, J., SchUdkraut, 1. and Anderson, J. E. (1994). Expression, purification, and crystallization of restriction endonuclease Pvull with DNA containing its recognition site. Proteins 19,77-79. 

Logue SE, Elgendy M, Martin SJ (2009) Expression, purification and use of recombinant annexin V for the detection of apoptotic cells. Nat Protoc 4 1383-1395... 

Ruppert, M., Panjikar, S., Barleben, L. and Stockigt, J. 2006. Heterologous expression, purification, crystallization and primary X-ray analysis of raucaffricine glu-cosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 62 257-260. 

For proteins that are members of gene families, such as cytochrome P450, available structural models derived from X-ray crystallography and NMR data allow inference of the structure of newly discovered family members. The inference is based on the sequence variations derived from comparing the reference and new DNA sequences. While a high degree of computational power is needed, predictions based on this approach provide preliminary estimates of protein structure without a huge investment in expression, purification, and crystallization. A full structural elucidation requires several years for each protein, even if it can be crystallized. 

Sinha U, Hancock T, Lin P-H, Hollenback S, Wolf D. Expression, purification, and characterization of inactive human coagulation factor Xa (Asn322Ala419). Protein Expr Purif 1992 3 518-524. 

Aoki, M. Iwamoto-Sugai, M. Sugiura, L Sasaki, C. Hasegawa, T. Oku-mura, C. Sugio, S. Kohno, T. Matsuzaki, T. Expression, purification and crystallization of human T-protein kinase I/glycogen synthase kinase-3. Acta Crystallogr. Sect. D, 56 (Pt 11), 1464-1465 (2000)... 

S.J. Ellington, W.R. Chapman, M.S. Expression, purification from inclusion bodies, and crystal characterization of a transition state analog complex of arginine kinase a model for studying phosphagen kinases. Protein Sci., 6, 444-449 (1997)... 

King DJ, Adair JR, Angal S, Low DC, et al. 1992. Expression, purification and characterization of a mouse-human chimeric antibody and chimeric Fab fragment. Biochem J. 281 317-323. 

J. Alam, N. Beyer, and H. w. Liu, Biosynthesis of colitose Expression, purification, and mechanistic characterization of GDP-4-keto-6-deoxy-D-mannose-3-dehydrase (ColD) and GDP-L-colitose synthase (ColC), Biochemistry, 43 (2004) 16450-16460. 

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