Protein expression/purification

Chen, H. Wang, R. Cerniglia, C. E. Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis. Protein Expression Purif. 2004,34, 302-310. 

Williams MD, Fieno AM, Grant RA, Sherman DH (1996) Protein Expression Purif 7 203... 

Nilsson, J., Stahl, S., Lundeberg, J., Uhlen, M., and Nygren, P.-A. (1997). Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expression Purif., 11, 1-16. 

Miki Y, Morales M, Ruiz-Duenas FJ et al (2009) Escherichia coli expression and in vitro activation of a unique ligninolytic peroxidase that has a catalytic tyrosine residue. Protein Express Purif 68 208-214... 

Spitsberg, V.L., Gorewit, R.C. 1998. Solubilization and purification of Xanthine oxidoreductase from bovine milk fat globule membrane. Protein Expression Purif. 13, 229-234. 

Geisse S, Gram H, Kleuser B, Kocher HP. Eukaryotic expression systems A comparison. Protein Express Purific 1996 8 271-82. 

HUANG, E. X., HUANG, Q. L WILDUNG, M. R CROTEAU, R SCOTT, A. I. Overproduction, in Escherichia coli, of soluble taxadiene synthase, a key enzyme in the taxol biosynthetic pathway, Protein Expression Purification, 1998,13,90-%. 

Gaskin, D. K., Bohach, G. A., Schlievert, P. M., and Hovde, C. J. (1997). Purification of Staphylococcus aureus /3-toxin Comparison of three isoelectric focusing methods. Protein Express. Purif. 9, 76-82. 

McDonald, J. R., Ong, M., Shen, C., Parandoosh, Z., Sosnowski, B., Bussel, S., and Houston, L. L. (1996). Large scale purification and characterization of recombinant fibroblast growth factor-saporin mitotoxin. Protein Express. Purif. 8, 97-108. 

Saxena, A., Chun man, L., Doctor, B.P. (2008). Developing procedures for the large-scale purification of human serum butyrylcholinesterase. Protein Express. Purif. 61 191-6. 

Roessner CA, Spencer JB,Ozaki S,Min CH,Atshaves BP,Nayar P,AnousisN, Stolowich NJ, Holderman MT, Scott AI (1995) Protein Express Purif 6 155... 

Magnuson, N.S. Linzmaier, P.M. Reeves, R. An, G. HaGlass, K. Lee, J. Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture. Protein Express Purif 1998, 13, 45-52. 

Hasslacher M, Schall M, Hayn M, Bona R, RumboJd K, Liickl J, Griengl H, Kohlwein SD, Schwab H (1997) Protein Express Purif 11 61... 

For biochemical assays with protein target reagents, one needs to only multiply the final concentrations of key reagents to estimate the total amount sufficient for the HTS project see Note 1). It is assumed that the infrastructure for protein expression, purification, and QC are within the HTS lab or accessible either through other core services or on an outsourced basis. 

Makrides, S.C. (1999) Components of vectors for gene transfer and expression in mammalian cells. Protein Express. Purif 17 183-202. 

Reeves R, An GH, Lee JM (1996) Enhanced recovery of a secreted mammalian protein from suspension culture of genetically modified tobacco cells. Protein Express Purif 7 220-228. 

Shin JH, Magnuson NS, Lee JM (2000) Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Protein Express Purif 19 131-138. 

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