Escherichia coli detection

D. M. Hatch, A. A. Weiss, R. R. Kale, and S. S. Iyer, Biotinylated bi- and tetra-antennary glycoconjugates for Escherichia coli detection, ChemBioChem, 9 (2008) 2433-2442. 

Bacterial pathogens are relatively large targets (> 1pm) and therefore, their presence can be detected directly with an optional amplification by secondary antibodies (sandwich assay). Examples of foodbome bacterial pathogens detected by SPR biosensors include Escherichia coli (detection limit 5x10 cfii/ml " " ), Listeria monocytogenes (detection limit 1 O cfii/ml " ) and Salmonella enteritidis (detection limit lO cfii/ml" ). 

The test is commonly employed as an initial screen for genotoxic activity and, in particular, for point mutation-inducing activity. It detects point mutations, which involve substimtion, addition or deletion of one or a few DNA base pairs. The reverse mutation test in either Salmonella typhimurium or Escherichia coli detects mutation in an amino acid requiring strain (histidine or tryptophan, respectively) to produce a strain independent of an outside supply of amino acid. The principle of the test is that it detects mutations, which revert mutations present in the test strains and restore the functional capability of the bacteria to synthesize an essential amino acid. The revertant bacteria are detected by their ability to grow in the absence of the amino acid required by the parent test strain. 

Ercole, C., Del Gallo, M., Mosiello, L., Baccella, S., and Lepidi, A. (2003). Escherichia coli detection in vegetable food by a potentiometric biosensor. Sens. Actmtors B Chem. 91, 163-168. 

Escherichia coli Detection by immunoassay with immunomagnetic bead [117]... 

Vogel J, Bartels V, Tang TH, Churakov G, Slagter-Jager JG, Huttenhofer A, Wagner EGH. RNomics in Escherichia coli detects new sRNA species and indicates parallel transcriptional output in bacteria. Nucleic Acids Res. 2003 31(22) 6435-6443. Kawano M, Reynolds AA, Miranda-Rios J, Storz G. Detection of 5 - and 3 -UTR-derived small RNAs and cis-encoded antisense... 

Kemp, D. J., Coppel, R. L., Cowman, A. F., Saint, R. B., Brown, G. V., and Anders, R. F. (1983). Expression of Plasmodium falciparum blood-stage antigens in Escherichia coli Detection with antibodies from immune humans. Proc. Natl. Acad. Sci. USA 80, 3787-3791. 

Escherichia coli detected in 10 ml coliform bacteria detected in 0.1 ml... 

Cheng, M.S., Lau, S.H., Chow, V.T. and Toh, C.-S. (2011) Membrane-based electrochemical nanobiosensor for Escherichia coli detection and analysis of cells viability Environ. Sci. Technol, 45,6453-6459. 

Escherichia coli (detection of 2-NP)° Commercial BDD, cleaning when passivated by 40 cycles from -tl.0Vto-l.7V range Amperometry Foodstuffs Tap water 400 cells mF ... 

Samadapour M, J Liston, JE Ongerth, PI Tarr (1990) Evalnation of DNA probes for detection of Shiga-like-toxin-producing Escherichia coli in food and calf fecal samples. Appl Environ Microbiol 56 1212-1215. 

In a study directed to the analysis of the role of Fe and the generation of H2O2 in Escherichia coli (McCormick et al. 1998), hydroxyl radicals were specihcally trapped by reaction with ethanol to give the a-hydroxyethyl radical. This formed a stable adduct with a-(4-pyridyl-l-oxide)-iV-t-butyl nitroxide that was not formed either by superoxide or hydroxyl radicals. The role of redox-reactive iron is to use EPR to analyze the EPR-detectable ascorbyl radicals. 

TNF is produced and secreted by activated cells within minutes following contact with LPS, reaching peak levels at 90-120 minutes after the admnistration of Escherichia coli endotoxin in human volunteers (M27). Van Deventer et al. (D15) could not detect serum TNF levels during experimental endotoxemia. Even during continuous intravenous administration of recombinant TNF (rTNF), serum TNF rapidly becomes undetectable (M27). It has been proposed that circulating soluble TNF receptors (sTNF-Rs) may be an important down-regulating mechanism (G10). 

Fortin, N. Y. Mulchandani, A. Chen, W. Use of real-time polymerase chain reaction and molecular beacons for the detection of Escherichia coli 0157 H7. Anal. Biochem. 2001, 289, 281-288. 

Pyle, B. H. Broadaway, S. C. McFeters, G A. Sensitive detection of Escherichia coli 0157 H7 in food and water by immunomagnetic separation and solid-phase laser cytometry. Appl. Environ. Microbiol. 1999, 65,1966-1972. 

Iqbal, S. Robinson, J. Deere, D. Saunders, J. R. Edwards, C. Porter, J. Efficiency of the polymerase chain reaction amplification of the uid gene for detection of Escherichia coli in contaminated water. Lett. Appl. Microbiol. 1997,24,498-502. 

Van Poucke, S. O. Nelis, H. J. Rapid detection of fluorescent and chemiluminescent total coliforms and Escherichia coli on membrane filters. J. Microbiol. Meth. 2000,42,233-244. 

Lekowska-Kochaniak, A. Czajkowska, D. Popowski, J. Detection of Escherichia coli 0157 H7 in raw meat by immunomagnetic separation and multiplex PCR. Acta Microbiol. Polon. 2002,51, 327-337. 

Sheridan, G. E. C. Masters, C. I. Shallcross, J. A. Mackey, B. M. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl. Environ. Microbiol. 1998, 64,1313-1318. 

Bej, A. K. McCarty, S. C. Atlas, R. M. Detection of coliform bacteria and Escherichia coli by multiplex polymerase chain reaction comparison with defined substrate and plating methods for water quality monitoring. Appl. Environ. Microbiol. 1991, 57, 2429-2432. 

Beilin, T. Pulz, M. Matussek, A. Hempen, H.-G. Gunzer, F. Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes. J. Clin. Microbiol. 2001,39, 370-374. 

Madonna, A. J. Van Cuyk, S. Voorhees, K. J. Detection of Escherichia coli using immunomagnetic separation and bacteriophage amplification coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Comm. Mass Spectrom. 2003,17, 257-263. 

Dai, Y. Li, L. Roser, D. C. Long, S. R. Detection and identification of low-mass peptides and proteins from solvent suspensions of Escherichia coli by HPLC fractionation and MALDI mass spectrometry. Rapid Comm. Mass Spectrom. 1999,13, 73-78. 

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