Detection of Escherichia coli and coliform bacteria

The cultivation of these germs is carried out in one process. Two methods can be employed  

Liquid enrichment with lactose-peptone solution 

Membrane-filter method using endoagar or endo-nutrient cardboard disks Liquid enrichment method 

Where the only question to be decided is whether or not Escherichia coli and/or coliform bacteria are present in 100 ml water, it is sufficient to 

With the second loop, part of the material is now spread out on a third of the culture medium surface at right angles to the first. Then the dish is turned through a further 20 and part of the material is applied with a third sterile loop to the part of the culture medium surface which has not yet been coated. It is possible in this way to obtain individual colonies, which can then be identified in the so-called colour series . 

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Sheet 3 - Detection of Escherichia coli and coliform bacteria... 

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