Detection of Escherichia coli

Fortin, N. Y. Mulchandani, A. Chen, W. Use of real-time polymerase chain reaction and molecular beacons for the detection of Escherichia coli 0157 H7. Anal. Biochem. 2001, 289, 281-288. 

Pyle, B. H. Broadaway, S. C. McFeters, G A. Sensitive detection of Escherichia coli 0157 H7 in food and water by immunomagnetic separation and solid-phase laser cytometry. Appl. Environ. Microbiol. 1999, 65,1966-1972. 

Iqbal, S. Robinson, J. Deere, D. Saunders, J. R. Edwards, C. Porter, J. Efficiency of the polymerase chain reaction amplification of the uid gene for detection of Escherichia coli in contaminated water. Lett. Appl. Microbiol. 1997,24,498-502. 

Lekowska-Kochaniak, A. Czajkowska, D. Popowski, J. Detection of Escherichia coli 0157 H7 in raw meat by immunomagnetic separation and multiplex PCR. Acta Microbiol. Polon. 2002,51, 327-337. 

Madonna, A. J. Van Cuyk, S. Voorhees, K. J. Detection of Escherichia coli using immunomagnetic separation and bacteriophage amplification coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Comm. Mass Spectrom. 2003,17, 257-263. 

Spangler B.D., Wilkinson E.A., Murphyb J.T., Tyler B.J., Comparison of the Spreeta surface plasmon resonance sensor and a quartz crystal microbalance for detection of Escherichia coli heat-labile enterotoxins, Analytica Chimica Acta 2001 444 149-161. 

Demarco D.R., Lim D.V., Detection of Escherichia coli 0157 H7 in 10-and 25-gram ground beef samples with an evanescent-wave biosensor with silica and polystyrene waveguides, J. Food Protect. 2002 65 596-602. 

Yu H., Bruno J.G., Immunomagnetic-electrochemiluminescent detection of Escherichia coli 0157 and Salmonella typhimurium in foods and environmental water samples, Appl. Environ. Micro. 1995 62 587-592. 

L. Yang, Y. Li, and G.F. Erf, Interdigitated array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli 0157 H7. Anal. Chem. 76, 1107—1113 (2004). 

H. Muramatsu, E. Tamiya, and I. Karube, Piezoelectric crystal biosensor system for detection of Escherichia coli. Anal. Lett. 22, 2155-2166 (1989). 

P. M. Fratamico, T. P. Strohaugh, M. B. Medina, and A. G. Gehring, "Detection of Escherichia coli 0157 H7 Using a Surface Plasmon Resonance Biosensor," Biotechnology Techniques 12, 571-576 (1998). 

Colquhoun, K. O., Timms, S., and Pricker, C. R. (1995). Detection of Escherichia coli in potable water using direct impedance technology, f. Appl. Bacteriol. 79, 635-639. 

DeMarco, D. R., Saaski, E. W., McCrae, D. A., and Lim, D. V. (1999). Rapid detection of Escherichia coli 0157 H7 in ground beef using a fiber-optic biosensor. /. Food Prot. 62, 711-716. 

Hahm, B. K., and Bhunia, A. K. (2006). Effect of environmental stresses on antibody-based detection of Escherichia coli 0157 H7, Salmonella enterica serotype Enteritidis and Listeria monocytogenes. J. Appl. Microbiol. 100,1017-1027. 

Oh, B. K., Lee, W., Lee, W. H., and Choi, J. W. (2003). Nano-scale probe fabrication using self-assembly technique and application to detection of Escherichia coli 0157 H7. Biotechnol. Bioprocess Eng. 8, 227-232. 

Prentice, N., Murray, J. S., Scott, M. F., Coombs, J. P., and Parton, A. (2006). Rapid isolation and detection of Escherichia coli 0157 H7 in fresh produce. /. Rapid Methods Automat. Microbiol. 14, 299-308. 

Ruan, C., Wang, H., and Li, Y. (2002a). A bienzyme electrochemical biosensor coupled with immunomagnetic separation for rapid detection of Escherichia coli 0157 H7 in food samples. Trans. ASAE 45,249-255. 

Glycolipid incorporated liposomes have found extensive use as sensors for the detection of Escherichia coli bacteria. Liposomes prepared using a diacetylene and a glucosyl lipid underwent a chromatic transition upon the addition of E. coli (Ma et al. 1998). The chromatic transition is sensitive to the diyne and glycolipid stmc-ture (Ma et al. 2000). An optimized vesicle assembly, consisting of a maltotriosyl lipid, phospholipid, and diyne, detected E. coli at a concentration of 2x10 cells/mL... 

Ma ZF, Li JR, Jiang L, Cao J, Boullanger P. Influence of the spacer length of glycolipid receptors in polydiacetylene vesicles on the colorimetric detection of Escherichia coli. Langmuir 2000 16 7801-7804. 

Rattray, E. A.S., Prosser, J. I., Killham, K. Glover, L. A. (1990). Luminescence-based nonextractive technique for in situ detection of Escherichia coli in soil. Applied and Environmental Microbiology, 56, 3368-74. 

Czajka, J. and Batt, C.A. (1996) A solid phase fluorescent capillary immunoassay for the detection of Escherichia coli 0157 H7 in ground beef and apple cider. Journal of Applied Bacteriology 81(6), 601-7. 

Work Item M horizontal method for the detection of Escherichia coli O 157. 

Jothikumar N, Griffiths MW (2002) Rapid detection of Escherichia coli 0157 H7 with multiplex real-time PCR assays. Appl Environ Microbiol 68 3169-3171... 

P20/I55.3 All foods Detection of Escherichia coli 0157 AFNOR-DYN-16/1 -03/96 Dynabeads anti Escherichia coli 0157... 

Several studies have focused on the specific detection of Escherichia coli with SPC, using different labelling procedures. While Lepeuple et al. (2003) used a 16S rRNA directed nucleic acid probe, Prescott and Fricker (1999) used a PNA probe. In both methods TSA was included to increase the fluorescence intensity. Van Poucke and Nelis (2000a, b) on the other hand used an enzyme substrate (fluorescein-6-D-diglucuronide) for the demonstration of 6-glucuronidase activity in Escherichia coli. The quantitative difference in enzyme activity between target and non-target bacterial cells allowed for the specific detection of Escherichia coli. 

Pyle et al. (1999) used SPC for the rapid detection of Escherichia coli 0157 H7 in meat. IMS allowed the isolation of target cells from the food matrix and was followed by a double labelling, using green fluorescent FTTC-conjugated antibodies and the red fluorescent viability substrate CTC. 

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