Immunomagnetic separation

Enzyme-linked immunosorbent assays (ELISA) Immunofluorescence assays (IFAs) Immunomagnetic separation... 

When the bacteria to be detected are less than 1% of the total population in a sample, IFAs cannot be used because of interference from unrelated particles that are concentrated when large volumes of sample are filtered. To overcome this problem, the organism of interest may be concentrated by immunomagnetic separation.10,51 62 For this procedure magnetic beads coated with monoclonal or polyclonal antibodies are mixed with the sample. The beads are collected with a magnet, and the cells attached to the beads then are removed, enumerated, and identified by IFAs. 

Pyle, B. H. Broadaway, S. C. McFeters, G A. Sensitive detection of Escherichia coli 0157 H7 in food and water by immunomagnetic separation and solid-phase laser cytometry. Appl. Environ. Microbiol. 1999, 65,1966-1972. 

Mansfield, L. P Forsythe, S. J. The detection of Salmonella using a combined immunomagnetic separation and ELISA end-detection procedure. Lett. Appl. Microbiol. 2000,31, 279-283. 

Lekowska-Kochaniak, A. Czajkowska, D. Popowski, J. Detection of Escherichia coli 0157 H7 in raw meat by immunomagnetic separation and multiplex PCR. Acta Microbiol. Polon. 2002,51, 327-337. 

Madonna, A. J. Van Cuyk, S. Voorhees, K. J. Detection of Escherichia coli using immunomagnetic separation and bacteriophage amplification coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Comm. Mass Spectrom. 2003,17, 257-263. 

Figure 14.6 Immunomagnetic separation (IMS) MALDI-MS protocol to isolate bacteria from biological mixtures.

El. Enroth, H., and Engstrand, L., Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens. J. Clin. Microbiol. 33, 2162-2165 (1995). 

Duncanson, P., Wareing, D. R. A., and Jones, O. (2003). Application of an automated immunomagnetic separation-enzyme immunoassay for the detection of Salmonella spp. during an outbreak associated with a retail premises. Lett. Appl. Microbiol. 37,144-148. 

Favrin, S. J., Jassim, S. A., and Griffiths, M. W. (2001). Development and optimization of a novel immunomagnetic separation-bacteriophage assay for detection of Salmonella enterica serovar Enteritidis in broth. Appl. Environ. Microbiol. 67, 217-224. 

Fegan, N., Higgs, G., Vanderlinde, P., and Desmarchelier, P. (2004). Enumeration of Escherichia coli 0157 in cattle faeces using most probable number technique and automated immunomagnetic separation. Lett. Appl. Microbiol. 38,56-59. 

Hudson, J. A., Lake, R. J., SaviU, M. G., Scholes, P., and McCormick, R. E. (2001). Rapid detection of Listeria monocytogenes in ham samples using immunomagnetic separation followed by polymerase chain reaction. /. Appl. Microbiol. 90, 614-621. 

Jacobsen, C. N., Fremming, C., and Jakobsen, M. (1997). Immunomagnetic separation of Listeria monocytogenes for flow cytometric determination of viable cells in liquid. /. Microbiol. Methods 31,75-81. 

Jenikova, G., Pazlarova, J., and Demnerova, K. (2000). Detection of Salmonella in food samples by the combination of immunomagnetic separation and PCR assay. Int. Microbiol. 3, 225-229. 

Jordan, D., Vancov, T., Chowdhury, A., Andersen, L. M., Jury, K., Stevenson, A. E., and Morris, S. G. (2004). The relationship between concentration of a dual marker strain of Salmonella typhimurium in bovine faeces and its probability of detection by immunomagnetic separation and culture. /. Appl. Microbiol. 97,1054-1062. 

Mercanoglu, B., and Aytac, S. A. (2002). Immunomagnetic separation and a cultural reference method for detection of Salmonella spp. in foods. Archiv Lebensmittelhyg. 53,43—45. 

Mercanoglu, B., and Griffiths, M. W. (2005). Combination of immunomagnetic separation with real-time per for rapid detection of Salmonella in milk, ground beef, and alfalfa sprouts. J. Food Prot. 68, 557-561. 

Reinders, R. D., Bama, A., Lipman, L. J. A., and Bijker, P. G. H. (2002). Comparison of the sensitivity of manual and automated immunomagnetic separation methods for detection of Shiga toxin-producing Escherichia coli 0157 H7 in milk. J. Appl. Microbiol. 92,1015-1020. 

Rijpens, N., Herman, L., Vereccken, F., Jannes, G., De Smedt, J., and De Zutter, L. (1999). Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCR. Int. J. Food Microbiol. 46,37M4. 

Ruan, C., Wang, H., and Li, Y. (2002a). A bienzyme electrochemical biosensor coupled with immunomagnetic separation for rapid detection of Escherichia coli 0157 H7 in food samples. Trans. ASAE 45,249-255. 

Skjerve, E., Rorvik, L. M., and Olsvik, O. (1990). Detection of Listeria monocytogenes in foods by immunomagnetic separation. Appl. Environ. Microbiol. 56,3478-3481. 

Varshney, M., Li, Y. B., Nanaparmeni, R., Johnson, M. G., and Griffis, C. L. (2003). A chemiluminescence biosensor coupled with immunomagnetic separation for rapid detection of Salmonella typMmurium.. Rapid Methods Automat. Microbiol. 11,111-131. 

Laundy, G J, Peberdy, M, Klouda, P T, and Bradley, A (1989) Applications of automated simultaneous double fluorescence (SDF). II HLA class I phenotypmg using immunomagnetically separated T-lymphocytes J Immunogenet 16, 141-148... 

Brun, A, Gaudernack, G, and Sandberg, S. (1990) A new method for isolation of reticulocytes positive selection of human reticulocytes by immunomagnetic separation Blood 76, 2397—2403... 

Cornelia, K., Nakamura, M., Melnik, K., Chosy, J., Zborowski, M., Cooper, M. A., et al. (2001) Effects of antibody concentration on the separation of human natural killer cells in a commercial immunomagnetic separation system. Cytometry 45, 285. 

CUDJOE K S, PATEL P D, OLSEN E, SKJERVE E and OLSVIK 0 (1993), Immunomagnetic separation techniques for the detection of pathogenic bacteria in foods , in Kroll R G, Gilmour A and Sussman M, New Techniques in Food and Beverage Microbiology, Oxford, Blackwell Scientific Publications, 17-31. 

The same platform was used to prepare BSA-functionalized magnetic MWNTs.32 After magnetic separation, those nanotubes with iron or iron oxide encapsulation were isolated and coated with anti-E coli 0157. The resulting immunomagnetic MWNTs were evaluated in immunomagnetic separation of E. coli 0157 H7 cells in pure and... 

Immunomagnetic separation using immunomagnetic beads coated with sheep antimouse IgC ... 

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