Doubled haploid

Smykalova, I., Smirous, P., Kubosiova, M., Gasmanova, N. and Griga, M. (2009). Doubled haploid production via anther culture in annual, winter type of caraway Carum carvi L.). Acta Physiologiae Plantarum, 31 (1) 21-31. 

The doubled haploids represent useful tools for applied breeding and genetic analysis. 

The utility of doubled haploid lines in barley breeding programs have been demonstrated, and a number of cultivars have been developed using this system. Microspore culture in barley has been improved more than in other cereals and thus is preferred for investigations such as microspore transformation. 

Both the stage of the microspore when collected for pretreatment and the pathway of nuclear development have also been considered to influence the frequency of doubling. He concluded that microspores collected at uninucleate stages 1-3 (early, mid and late, respectively) resulted in mostly haploid and doubled haploid plants while those collected at later stages (4-6, mitosis and binucleate) resulted in mostly doubled haploids as well as some triploid and tetraploid plants. It has also been demonstrated in wheat that the pretreatment method will influence the pathway alongwhich the nuclei will develop. 

Fig. 2. Cytogenetic test for androgenetic plantlets in barley. The majority of plantlets were spontaneous doubled haploid (A) and Low percents of them were haploid (B). Kahrizi D (2009).

Liu W, Zheng MY, Polle EA. Konzak C.F. (2002). Highly efficient doubled-haploid production in wheat Triticum aestivum L.) via induced microspore embryogenesis. Grop Sci. 42 686-692. 

Barnabas B. 2003a. Protocol for producing doubled haploid plants from anther culture of wheat (Tritkum aestivum L.). In Maluszynaki M, Kasha KJ, Forster BP, Szarejko I. 

Cistue L, Riomas A, Castillo AM and Romagosa L. 1994. Production of a large number of doubled haploid plants from barley anthers protected with high concentration of mannitol. Plant Cell Report 13 709 - 712. 

Germana MA. 2011. Anther culture for haploid and doubled haploid production. Plant Cell Tissue and Organ Culture 104 283 - 300. 

Grewal RK, Lulsdorf M, Croser J, Ochatt S, Vandenberg A and Warkentin TD. 2009. Doubled haploid production in chick pea (Cicer arietinum L.) role of stress treatments. Plant Cell Rep 28 1289 -1299. 

Maluszynski M, Kasha KJ, Szarejko I 2003. Published double haploid protocols in plant species. In Maluszynski M, Kasha K, Forster BP, Szarejko I (eds.). Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, pp 309 - 335. 

Progress in doubled haploid technology in higher plants. In Touraev A, Forster BP, Jain SM, (eds.). Advances in haploid production in higher plants. Spainger, Berlin, pp 1 - 34. 

HPLC is also useful in genetic studies of other cereals. Devaux et al. [174] used RP-HPLC to analyze B- and C-hordeins from barley doubled haploid lines... 

Alkaloids are one of the most important groups of secondary metabolites used since prehistoric age. It has been used as an anti-inflammatory agent for a long time. Besides, as a potential mitotic poison, colchicine is applied to produce polyploid and double haploid plants by the plant breeders. Colchicine is... 

Alkaloid anti-inflammatory agent autumnaline colchicine Colchicum double haploid elicitors in vitro culture phenethylisoquinoline polyploid... 

Botanical Uses of Colchicine 6.2.1 Development of Double Haploid Lines... 

Redha A, Attia T, Buter B, Saisingtraig S, Stamp P, Schmid JE (1998) Improved prodijction of doubled haploids by colchicine application to wheat (J riticum aestivum) anther culture. Plant Cell Rep 17 974-979... 

Wan Y, Petolino JF, Widholm JM (1989) Efficitait production of doubled haploid plants through colchicine treatment of anther derived maize callus. Theor AppI Genet 77 889-892... 

Detection, 1967 program, 2409 window, 1082 Detoxification, 2395 Detrusor smooth muscle, 3992 Devirilization, 2418 Dexamethasone, 4119,4120, 4147 Dexterity, 2414 DH. See Double haploid (DH)... 

We have obtained a double haploid mutant line of B. napus that is similar in lipid and fatty add composition at 2 C but which shows lower levels of chloroplast lipids and trienoic acids (18 3 and 16 3) at low temperature (5 C). With the low trienoic fatty acids there is a concomitant increase in the dienoic fatty acids. This is the reverse of the normal situation where plants grown at constant low temperature increase the level of trienoic fatty acids. 

Similar Imes have also been isolated from double haploid mutant lines that show a correlation between the level of 18 3 in PC at 5 C and the 18 3 level of the seed oil (2.5-12 wt%, measured at approx. 20°C). These multiple mutants respond in varying degrees to low temperature induction of desaturation in PC in the leaves and appear to effect the level of 18 3 in the seed oil. 

St T was a double haploid line of Stellar. Certified seeds of the French variety Drakkar (Drk C) were used as high linolenic acid control. 

Amar S, Becker HC, MoUers C. 2008. Genetic Variation and Genotype X Environment Interactions of Phytosterol Content in Three Doubled Haploid Populations of Winter Rapeseed. Crop Sci. 48 1000-1006. 

Salmanowicz BP, Surma M, Adamski T, R barz M 2008. Effects of amounts of HMW glutenin subunits determined by capillary electrophoresis on technological properties in wheat doubled haploids. J Sci Food Agric 88 1716-1725. 

Tang, J., R. Scarth and P.B.E. McVetty (2004), Stability of the Expression of Acyl-ACP Thioesterase Transgenes in Oilseed Rape Doubled Haploid Lines , Crop Science Vol. 44, pp. 732-740. 

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