Diode array detector capillary electrophoresis

A capillary electrophoresis systems Agilent CE 1100 (HP, USA) equipped with a diode array detector was used to separate and quantify... [Pg.150]

AMPA = aminomethylphosphoric acid, CZE = capillary zone electrophoresis, DAD = = diode array detector. MEKC =... [Pg.745]

Capillary electrophoresis with diode array detection. Ultraviolet spectra (190-350 nm) were recorded after separation of fractions III, IV, and V-2 by capillary electrophoresis with diode array detection (CE-DAD) in 30 mM phosphoric acid buffer pH 2.3 and pH 7.2. Separations were carried out with a Hewlett-Packard 3D GE equipped with a diode array detector. Samples were introduced under pressure (30 mbar, 10 sec) into a fused silica capillary (effecfive lengfh 8 cm, total 64.5 cm, ID 50 pm, OD 375 pm) followed by separafion af 30 kV. [Pg.78]

Every analyst who has access to CE equipment and ESI-MS may take this new alternative into consideration when appropriate. When is it appropriate If an analytical problem can be solved using affinity capillary electrophoresis and a more universal and specific detection or structural information is required than is accessible with the help of conventional UV diode array detectors, consider ACE/MS. [Pg.355]

Both Parts I and II have been completely rewritten and reflect the many advances in biochemistry-molecular biology theory and techniques. Especially noteworthy have been the technical advances in chromatography (perfusion, FPLC, bioaffinity), electrophoresis (pulsed gel, capillary, nucleic acid sequencing), spectrophotometry (nmr, ms, and diode array detectors), and molecular biology (microsequencing of proteins and nucleic acids, blotting, restriction enzymes). [Pg.483]

Notes LOD, limit of detection MeOH, methanol EtOH, ethanol ACN, acetonitrile EtAC, ethyl acetate SPE, solid phase extraction HLB (hydrophilic lipophilic balanced) TFA, trifluoroacetic acid GC, gas chromatography TMS, trimethylsilyl MS, mass spectrometry HPLC, high-performance liquid chromatography DAD, diode array detector NMR, nuclear magnetic resonance ESI, electrospray ionization APCI, atmospheric pressure chemical ionization CE, capillary electrophoresis ECD, electrochemical detector CD, conductivity detector TLC, thin layer chromatography PDA, photodiode array detector. [Pg.65]

Jimenez-Lozano E., Marques I., Barrdn D., Beltran J.L., Barbosa J., Determination of pATa values of quinolones from mobdity and spectroscopic data obtained by capillary electrophoresis and a diode array detector. Anal. Chim. Acta, 464, 37- 5 (2002). [Pg.180]

Abbreviations DAD, diode array detector FIC, flow injection chromatography FIA-SPE-MEKC, flow injection analysis-solid phase extraction-micellar electro kinetic chromatography HPLC-FI-CH, high performance liquid chromatography-flow injection-chemiluminescence FI-CH, flow injection-chemiluminescence detection FIPCE, high performance capillary electrophoresis LOD, limit of detection LOQ, limit of quantitation SIC, sequential injection chromatography. [Pg.185]

PG propyl gallate OG octyl gallate DG dodecyl gallate TBHQ BHA butylated hydroxyanisole BHT butylated hydroxytoluene LC capillary electrophoresis and DAD diode array detector. [Pg.250]

For many applications, diode array detection has become routine. A photodiode array was used for simultaneous detection of 100 capillaries in zone electrophoresis and micellar electrokinetic chromatography (MEKC).1516 Deflection of a laser beam by acoustic waves was reported as a means to scan six capillary channels on a microchip.17 The design of a low-noise amperometric detector for capillary electrophoresis has been reported.18... [Pg.428]

The most important applications for diode array systems are in molecular spectroscopy, since in general they do not have the resolution necessary for atomic spectroscopy. In molecular spectroscopy the most useful areas of application are for (1) scanning fast reactions to determine kinetics, (2) applications involving low light levels because spectra can be stored and added to each other, increasing the intensity, and (3) detectors for HPLC and capillary electrophoresis (CE). HPLC and CE are discussed in Chapter 13. [Pg.341]

Online applications are by far the most important utilization of diode array spectrometry. High-performance liquid chromatography, supercritical fluid chromatography, capillary electrophoresis, and flow-injection techniques produce enhanced sensitivity and structure-related information due to coupling with diode-array-based detectors. Emission of the microwave-induced plasma generated in atomic emission detectors for capillary gas chromatography is also analyzed by means of UV-Vis diode array instruments. [Pg.4473]

Detection methods HPLC-DAD, HPLC with diode-array detection. HPLC-(ESI-MS), HPLC with electrospray ionization mass spectrometry. RP-HPLC, reversed-phase HPLC. UPLC/Q-TOFMS, UPLC with quadrupole-time of flight mass spectrometry. HPLC-APCI-MS, HPLC with atmospheric pressure chemical ionization mass spectrometry. CE-IT-MS, capillary electrophoresis-ion-trap mass spectrometry. LC-ESI-ITMS, liquid chromatography-electrospray ionization ion trap mass spectrometry. LC-ELSD, LC with evaporative light scattering detector. DNBZ-Cl, 3,5-dinitrobenzoyI chloride. [Pg.294]