Capillary electrophoresis-diode array

Mateus, L., Cherkaoui, S., Christen, R, and Veuthey, J. L. (1999). Capillary electrophoresis-diode array detection-electrospray mass spectrometry for the analysis of selected tropane alkaloids in plant extracts. Electrophoresis 20, 3402—3409. 

Sato S, Soga T, Nishioka T, Tomita M. Simultaneous deter- 55. mination of the main metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection. Plant J. 2004 40 151-163. 

Bianco A, Uccella N (2000) Biophenolic components of olives. Food Res Int 33 475 85 Savarese M et al (2007) Characterization of phenolic extracts from olives (Olea europaea cv. Pisciottana) by electrospray ionization mass spectrometry. Food Chem 105 761-770 Ryana D et al (1999) Determination of phenolic compounds in olives by reversed-phase chromatography and mass spectrometry. J Chromatogr A 832 87-96 Priego-Capote F et al (2004) Fast separation and determination of phenolic compounds by capillary electrophoresis-diode array detection application to the characterization of alperujo after ultrasound-assisted extraction. J Chromatogr A 1045 239-246... 

Priego-Capote, F., Ruiz-Jimenez, J., and Luque de Castro, M.D., Fast separation and determination of phenolic compounds by capillary electrophoresis-diode array detection. Application to the characterisation of alperujo after ultrasound-assisted extraction, J. Chromatogr. A, 1045, 239, 2004. 

A capillary electrophoresis systems Agilent CE 1100 (HP, USA) equipped with a diode array detector was used to separate and quantify... 

AMPA = aminomethylphosphoric acid, CZE = capillary zone electrophoresis, DAD = = diode array detector. MEKC =... 

For many applications, diode array detection has become routine. A photodiode array was used for simultaneous detection of 100 capillaries in zone electrophoresis and micellar electrokinetic chromatography (MEKC).1516 Deflection of a laser beam by acoustic waves was reported as a means to scan six capillary channels on a microchip.17 The design of a low-noise amperometric detector for capillary electrophoresis has been reported.18... 

M. Trojanowicz, L. Wojcik and K. Urbaniak Walczak, Identification of natural dyes in historical Coptic textiles by capillary electrophoresis with diode array detection, Chem. Anal. (Warsaw), 48, 607 620 (2003). 

R. Loos, M.C. Alonso and D. Barcelo, Solid-phase extraction of polar hydrophilic aromatic sulfonates followed by capillary zone electrophoresis-UV absorbance detection and ion-pair liquid chromatography-diode array UV detection and electrospray mass spectrometry. J. Chromatogr.A 890 (2000) 225-237. 

Capillary electrophoresis with diode array detection. Ultraviolet spectra (190-350 nm) were recorded after separation of fractions III, IV, and V-2 by capillary electrophoresis with diode array detection (CE-DAD) in 30 mM phosphoric acid buffer pH 2.3 and pH 7.2. Separations were carried out with a Hewlett-Packard 3D GE equipped with a diode array detector. Samples were introduced under pressure (30 mbar, 10 sec) into a fused silica capillary (effecfive lengfh 8 cm, total 64.5 cm, ID 50 pm, OD 375 pm) followed by separafion af 30 kV. 

Capillary electrophoresis ofV-2 at pH 2.3 and pH 7.2. Inserts ultraviolet spectra (190-350 nm) of selected fractions. Capillary separation at -30kV with diode array detection. 

Every analyst who has access to CE equipment and ESI-MS may take this new alternative into consideration when appropriate. When is it appropriate If an analytical problem can be solved using affinity capillary electrophoresis and a more universal and specific detection or structural information is required than is accessible with the help of conventional UV diode array detectors, consider ACE/MS. 

Both Parts I and II have been completely rewritten and reflect the many advances in biochemistry-molecular biology theory and techniques. Especially noteworthy have been the technical advances in chromatography (perfusion, FPLC, bioaffinity), electrophoresis (pulsed gel, capillary, nucleic acid sequencing), spectrophotometry (nmr, ms, and diode array detectors), and molecular biology (microsequencing of proteins and nucleic acids, blotting, restriction enzymes). 

Notes LOD, limit of detection MeOH, methanol EtOH, ethanol ACN, acetonitrile EtAC, ethyl acetate SPE, solid phase extraction HLB (hydrophilic lipophilic balanced) TFA, trifluoroacetic acid GC, gas chromatography TMS, trimethylsilyl MS, mass spectrometry HPLC, high-performance liquid chromatography DAD, diode array detector NMR, nuclear magnetic resonance ESI, electrospray ionization APCI, atmospheric pressure chemical ionization CE, capillary electrophoresis ECD, electrochemical detector CD, conductivity detector TLC, thin layer chromatography PDA, photodiode array detector. 

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