Data from HPLC

According to Section 4.5.3 concentration data can also be obtained by chromatography. 

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FIGURE 9.1 van Deemter plots for chromatographic data from HPLC and SFC elution of... 

Data from HPLC, MS, NMR and IR have to be stored in standard analytical file formats and transferred to a generic data viewer connected to standard databases that are valuable in drug discovery. 

Procedures for statistical evaluation of analytical data from HPLC (and other chromatographic and electrophoretic methods) have been reviewed by Bietz and Simpson [91], The reader is also referred to an excellent article by Scanlon [92] that discusses this topic in detail. 

Data from HPLC demonstrates that BSA is effective as chiral selector when the [BSA]/[solute] ratio is greater than 1. 

Direct instrument control (or the lack of it) was an important issue for the earlier version of CDS. The scheme of connecting the detector channels through A/Ds to CDS worked well in analytical laboratories across the pharmaceutical industry. The scheme provided enough flexibility so that the CDS could collect data from a variety of instruments, including GC, HPLC, IC, SFC, and CE. It was equally important that the CDS could be connected to instruments that were manufactured by different vendors. It was not uncommon to find a variety of instruments from different vendors in a global pharmaceutical research company. The disadvantage of this scheme was that the instrument metadata could not be linked to the result file of each sample analyzed. It could not be guaranteed that the proper instrument parameters were used in sample analysis. Another need came from the increased use of... 

Data from Hasegawa et at. (1992). Determined by hplc on an optically active phase, "rt = room temperature. 

Nonvolatile Nitrosamines In Tobacco. A method which we developed several years ago for the analysis of tobacco-specific nitrosamines (TSNA 31) involves extraction of tobacco with buffered ascorbic acid TpH 4.5) followed by partition with ethyl acetate, chromatographic clean-up on silica gel, and analysis by HPLC-TEA (Figure 9). Results obtained with this method for a large spectrum of tobacco products (Table IV), strongly support the concept that the levels of nitrate and alkaloids, and especially the methods for curing and fermentation, determine the yields of TSNA in tobacco products. Recent and as yet preliminary data from snuff analyses indicate that aerobic bacteria play a role in the formation of TSNA during air curing and fermentation. 

From HPLC retention data (Swann et al. 1983, Szabo et al. 1990). 

Tomlinson, E., Hafkenscheid, T. L. (1986) Aqueous solution and partition coefficient estimation from HPLC data. In Partition Coefficient, Determination and Estimation. Dunn, III, W. J., Block, J. H., Pearlman, R. S., Eds., pp. 101-141, Pergamon Press, New York. 

Multiple sprayer ion source with rotatory ion beam chopper (MUX) (performed by instrument maker) Place two to four sprayers in same interface housing each sprayer receives effluent of HPLC spinning chopper protects orifice of MS to allow ions only from one sprayer to enter at a time special MS program separates data from sprayers HPLCs operated in parallel and simultaneously... 

The flow rate was 1 ml/min and AB25 was detected at 600 run (retention time, 10.2 min). LC-MS was performed in an ODS column (150 x 3mm i.d. particle size 3 /tin) using gradient elution from 0 to 100 per cent A in 25 min. MS measurements were made with ESI in negative mode. It can be concluded from the data that HPLC-DAD and HPLC-MS offer a unique possibility for the investigation of the photocatalytic degradation of Acid blue 25 and make possible the identification of coloured intermediates [126],... 

Strohschein, S., Pursch, M., Lubda, D., and Albert, K., Shape selectivity of C-30 phases for RP-HPLC separation of tocopherol isomers and correlation with MAS NMR data from suspended stationary phases. Anal. Chem., 70, 13, 1998. 

In the late 1970s, Hewlett-Packard introduced the HP-3300 series data-acquisition system, which was able to connect to 60 chromatographic instruments through an A/D converter. This was the beginning of what would become a revolution in CDS development within the analytical instrument industry. By the mid-1980s, all of the major analytical instrument manufacturers offered network-based data-acquisition systems Beckman, HP, PE, VG, and Waters. These were multi-user, time-sharing systems that used A/D converters to acquire data from the instruments. Instrument control, both HPLC and GC, was a capability that would soon follow. Several CDS manufacturers offered serial control of the HP 5890 GC while Waters also offered instrument control for their own HPLCs. 

Appropriate system suitability is very important for CE assays in a QC environment. It is preferable to define CE-specific system suitability criteria rather than directly adopt system suitability based on HPLC methods. Earlier data from biotech companies showed a high assay failure rate due to inappropriate system suitability criteria. When appropriate criteria were applied in the QC environment, the assay failure rate improved. 

Fig. 2.18 Raw data from a model GPC spin column/microbore HPLC ESI-MS primary screen of 400 compounds with PKA protein spiked with both staurosporine and olomoucine, known ligands of PKA. (Left) TIC, UV trace at 214 nm, and corresponding mass chromatograms for olomoucine and...

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