Cord blood progenitors

Noirey, N., Rougier, N., Andre, C., Schmitt, D., and Vincent, C. 2000. Langerhans-like dendritic cells generated from cord blood progenitors internalize pollen allergens by macro-pinocytosis, and part of the molecules are processed and can activate autologous native T lymphocytes. J Allergy Clin Immunol 105 1194-1201. 

Chen N, Hudson J E, Walczak P, et al. (2005). Human umbilical cord blood progenitors The potential of these hematopoietic cells to become neural. Stem Cells. 23 1560-1570. 

Daculsi R. Remy-Zolghadri M, Grellier M, Conrad V, Fernandez P, Bareille R, Bordenave L. Signal transduction and procoagulant state of human cord blood-progenitor-derived endothelial cells after interleukin-lalpha stimulation. Endothelium 14 163-171,2007. 

Studies on expression of cysteinyl leukotriene receptors by human mast cells unexpectedly revealed that LTE4 helps to induce greater numbers of mast cells from cord blood progenitor cells cultured together with lL-6 and IL-10 than... 

Koller, M. R., J. G. Bender, E. T. Papoutsakis, and W. M. MiUer. 1992. Effects of synergistic cytokine combinations, low oxygen, and irradiated stroma on the expansion of human cord blood progenitors. Blood 80(2) 403-11. 

Compare the different types of HCTs, specifically (a) the types of donors (i.e., autologous and allogeneic), (b) the source of hematopoietic cells (i.e., umbilical cord, peripheral blood progenitor cells, and bone marrow), and (c) the type of preparative regimen (i.e., myeloablative and nonmyeloablative). 

Umbilical cord blood, peripheral blood progenitor cells (PBPC) and bone marrow can serve as the source of hematopoietic cells. The optimal cell source differs based on the donor and recipient characteristics but has not been clearly identified for all patients. 

The type of HCT performed depends on a number of factors, including type and status of disease, availability of a compatible donor, patient age, performance status, and organ function. In addition to bone marrow, hematopoietic cells may be obtained from the peripheral blood progenitor cells (PBPCs) and umbilical cord blood. The essential properties of the hematopoietic cells are their ability to engraft, the speed of engraftment, and the durability of engraftment.1... 

Christopherson KW 2nd, Hangoc G, Broxmeyer HE. Cell surface peptidase CD26/ dipeptidylpeptidase IV regulates CXCL12/stromal cell-derived factor-1 alpha-mediated chemotaxis of human cord blood CD34+ progenitor cells. J Immunol 2002 169(12) 7000-7008. 

Mayani H, Lansdorp PM, 1998. Biology of human umbilical cord blood-derived hematopoietic stem/progenitor cells. Stem Gells 16 153... 

Theunissen, K. Verfaillie, C.M. (2005) A multifactorial analysis of umbilical cord blood, adult bone marrow and mobilized peripheral blood progenitors using the improved ML IC assay. Experimental Hematology, 33,165-172. 

Third type of culture is stroma-non contact . In this system primitive progenitor cells are sustained when cells are co-cultured with irradiated allogeneic stroma but separated from it by the 0,4 micron membrane in transwell inserts (Costar, Cambridge, MA). These cultures are maintained by daily supplementation of stromal feeder conditioned media (Roller et al. 1998, Verfaillie, 2001) successfiilly expanded umbilical cord blood cells in a novel automated perfusion culture system. Development these approaches followed in the studies of investigators who incorporated the stromal components into the expansion culture. Recently published trials by McNiece et al. 2000 are more encouraging where cells were expanded in static culture for 10 days in Teflon bags (American Fluoroseal, USA). 

Broxmeyer, H.E., Gordon, G.W., Hangoc, G. et al. (1989) Human umbilical cord blood as a potential source of transplantable hematopoietic stem/ progenitor cells, Proc. Natl. Acad. ScL t/S 86 3828-3832. 

Endothelial progenitor cells can be isolated from the mononuclear fraction of the bone marrow or peripheral blood, as well as from fetal liver or umbilical cord blood [12, 23, 66, 67], Heterologous,... 

Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol 2000 109 235-242. 

There are three sources of hematopoietic stem and progenitor cells available bone marrow (BM), peripheral blood after stem cell mobifization (MPB) and umbilical cord blood (CB). 

Other mechanistic approaches to cell therapy can also be envisioned, For example, a characteristic of compensated cardiac hypertrophy is the reduction of mechanical stress—a clear call for cells that are able to stabilize scar. Mesenchymal BM cells, fibroblasts, and skeletal myoblasts (all mesoderm-derived progenitors) improve diastolic function and reduce wall stress (30). The BN-MNC, peripheral blood endothelial progenitor cells (EPCs), and umbilical cord blood (UCB) cells... 

Umbilical cord blood is an alternative source of hemopoietic stem cells rather than bone marrow, especially for patients who lack an HLA-matched donor (35). Umbilical cord blood contains significantly more early and committed progenitor cells and has been used in patients with malignant and non-mahgnant hematological diseases. One of the advantages of cord blood is that it... 

Koller MR, Bender JG, Miller WM Papoutsakis ET (1992b) Reduced oxygen tension increases hematopoiesis in longterm culture of human stem and progenitor cells from cord blood and bone marrow. Experimental Hematology 20 264-270. 

I. Kashiwakura, and T.A. Takahashi, Basic fibroblast growth factor-stimulated ex vivo expansion of haematopoietic progenitor cells from human placental and umbilical cord blood, Br J Haematol 122, 479-488 (2003). 

Chua KN et al (2006) Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells. Biomaterials 27(36) 6043-6051... 

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