Chromatography novel applications

The structure of the second edition follows that of the first edition, with novel applications contained in sections attached to the individual chapters. The present chapter covers mass spectroscopy, with additional applications being found in the individual chapters, as well as advances in common detectors, unusual modes of chromatography, and general theoretical advances. 

High-temperature high-resolution gas chromatography (HTGC) is an established technique for the separation of complex mixtures of high molecular weight compounds (HMW) which do not elute when analyzed on conventional GC columns [530]. The combination of this technique with mass spectrometry (i.e., HTGC-MS) is not so common, however, Elias et al. [530] used this novel application to evaluate and identify the occurrence of HMW tracers (> C40) from smoke aerosols. 

Novel applications and new developments in gas chromatography. Patel-Predd, P. LC-GC Meeting Report from Pittcon 2006, March 2006. 

Cinchona alkaloids comprising quinine, quinidine, cinchonidine, and cinchonine as the major members constitute a unique class of quinoline alkaloids with tremendous impact on human civilization. The odyssey of Cinchona alkaloids began with the discovery of their antimalarial properties followed by the very successful application in stereochemistry and in asymmetric synthesis. Currently, the portfolio of applications of Cinchona alkaloids is much broader, involving chiral stationary phases for enantioselective chromatography, novel biological activities, and several useful transformation converting them into other modular and chiral building blocks, such as, for example, quincorine or quincoridine. Current pressure on a more intense exploration of sustainable products and easy access to diverse molecular architectures make Cinchona alkaloids of primary importance for synthetic catalytic and medicinal chemistry. 

McGuffin V L 1984 Novel applications of microcolumn liquid chromatography. LC Mag 2 282-288... 

Howard, A.J., G. Carla and C.H. Byers, "Novel applications of continuous atmular chromatography separation of sugars," ACS National Meeting, Denver, CO, April, 5-10 (1987). 

A third parameter to consider is the column construction. Thus the sample applicator should provide optimal sample application to give the most performance possible out of the packed bed. Constructions should also allow simple, fast, and reproducible packing of the column. Because costs for repacking of columns are a substantial operating cost item in industrial chromatography, the selection of column construction from this point of view is also important. Some novel column constructions allow very simple procedures both for laboratory and for industrial scale (e.g., INdEX columns, see Section V). 

The development of new fiber coatings in the near future should further improve the specificity of SPME and overcome some of the observed matrix effects. Quantification by stable isotope dilution gas chromatography/mass spectrometry (GC/MS) may assist in improving analytical performance. Along with the possible application of micro LC and capillary LC columns to in-tube SPME, the development of novel derivatization methods and the potential for the analysis of fumigant pesticides, SPME appears to be a technique with a future in the analysis of pesticide residues in food. 

Nowak, M. and Seubert, A., Application of experimental design for the characterization of a novel elution system for high capacity anion chromatography with suppressed conductivity detection, /. Chromatogr. A, 855, 91,1999. 

J Gerhardt, K Nokihara, R Yamamoto. Design and applications of a novel amino acid analyzer for D/L and quantitative analysis with gas chromatography, in JA Smith, JE Rivier, eds. Peptides Chemistry and Biology. Proceedings of the 12th American Peptide Symposium, Escom, Leiden, 1992, pp 457-458. 

Magner J, Filipovic M, Alsberg T (2010) Application of a novel solid-phase-extraction sampler and ultra-performance liquid chromatography quadrupole-time-of-flight mass... 

Wehmeyer KR, Kasting GB, Powell JH, Kuhlenbeck DL, Underwood RA, Bowman LA. Application of liquid-chromatography with online radiochemical detection to metabolism studies on a novel class of analgesics. Journal of Pharmaceutical and Biomedical Analysis 8, 177-183, 1990. 

Liquid chromatography/mass spectrometry (LC/MS)-based techniques provide unique capabilities for pharmaceutical analysis. LC/MS methods are applicable to a wide range of compounds of pharmaceutical interest, and they feature powerful analytical figures of merit (sensitivity, selectivity, speed of analysis, and cost-effectiveness). These analytical features have continually improved, resulting in easier-to-use and more reliable instruments. These developments coincided with the pharmaceutical industry s focus on describing the collective properties of novel compounds in a rapid, precise, and quantitative way. As a result, the predominant pharmaceutical sample type shifted from nontrace/pure samples to trace mixtures (i.e., protein digests, natural products, automated synthesis, bile, plasma, urine). The results of these developments have been sig-... 

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