Chip laser

HPLC with microchip electrophoresis. Capillary RPLC was used as the first dimension, and chip CE as the second dimension to perform fast sample transfers and separations. A valve-free gating interface was devised simply by inserting the outlet end of LC column into the cross-channel on a specially designed chip. Laser-induced fluorescence was used for detecting the FITC-labeled peptides of a BSA digest. The capillary HPLC effluents were continuously delivered every 20 s to the chip for CE separation. 

In order to provide access holes on fused quartz cover chips, laser drilling [151,152,828,1006] or ultrasonic drilling [349,1041] have been used. In order to remove particulates caused by drilling, the drilled quartz plate was rinsed in 0.5% HF for 5-10 min in an ultrasonic bath [153,658]. However, in some cases no drilling was performed, with the end of the channel protruding out of the coverslip [148],... 

Fig. 4 Examples of portable microfluidic instruments, (a) The system integrates fluidics, microseparation chips, lasers, optics, high-voltage power supplies, electronic controls, data algorithms, and a user interface into a hand-portable instrument (Reprinted from [55] with permission of The American Chemistry Society), (b) A miniature LED-induced fluorescence microdevice (Reprinted from [56] with permission of The Royal Society of Chemistry), (c) Hand-held isotachophoresis instrument (dimensions 7.6 x 5.7 x 3.8 cm) (Reprinted from [57] with permission of The Royal Society of Chemistry)...

The chip laser is actually a miniaturised version of the Nd-YAG laser. It contains a diode laser pump, an active laser medium, a saturable absorber, and a frequency multiplier in a solid block. Chip lasers are an inexpensive and reliable source of UV radiation. Unfortunately they cannot be made with repetition rates higher than a few tens of kHz. The pulse width is of the order of 1 ns. Chip lasers are sometimes used in TCSPC systems for environmental research, e.g. to trace contamination of water by polycyclic hydrocarbons. 

Because of the possibility of focusing laser beams, tlrin films can be produced at precisely defined locations. Using a microscope train of lenses to focus a laser beam makes possible tire production of microregions suitable for application in computer chip production. The photolytic process produces islands of product nuclei, which act as preferential nucleation sites for further deposition, and tlrus to some unevenness in tire product film. This is because the subsuate is relatively cool, and therefore tire surface mobility of the deposited atoms is low. In pyrolytic decomposition, the region over which deposition occurs depends on the drermal conductivity of the substrate, being wider the lower the thermal conductivity. For example, the surface area of a deposit of silicon on silicon is nanower dran the deposition of silicon on silica, or on a surface-oxidized silicon sample, using the same beam geomeU y. 

Ultraviolet coatings for excimer lasers. These lasers are likely to play a large role in lithography of very high density computer chips,... 

The capability to control an excimer laser beam also is exploited in the semiconductor industry, where these lasers are used to etch elaborate features during the fabrication of semiconductor chips. Neil Bartlett probably never dreamed that his explorations of the chemistry of xenon would lead to such exotic applications. 

The ProteinChip System from Ciphergen Biosystems uses patented SELDI (Surface-Enhanced Laser Desorption/Ionization) ProteinChip technology to rapidly perform the separation, detection, and analysis of proteins at the femtomole level directly from biological samples. ProteinChip Systems use ProteinChip Arrays which contain chemically (cationic, anionic, hydrophobic, hydrophilic, etc.) or biochemically (antibody, receptor, DNA, etc.) treated surfaces for specific interaction with proteins of interest. Selected washes create on-chip, high-resolution protein maps. This protein mass profile, or reten-tate map of the proteins bound to each of the ProteinChip Array surfaces, is quantitatively detected in minutes by the ProteinChip Reader. 

In a second experiment, Cy5-labelled antiBSA antibodies were immobilised on a silanised glass slide precoated with metallic nanoislands using a polydimethylsiloxane (PDMS) flow-cell. The antibody solution was left for 1 hour to attach and then the cell was flushed with deionised water. The slide was then dried with N2. For this experiment, a portion of the slide was not coated with metallic nanoislands, in order to act as a reference. Figure 20 shows the image recorded using the fluorescence laser scanner mentioned previously. The enhancement in fluorescence emission between those areas with and without nanoislands (B and A, respectively) is again evident. For both chips, an enhancement factor of approximately 8 was recorded. There is considerable interest in the elucidation and exploitation of plasmonic effects for fluorescence-based biosensors and other applications. 

Bowden M., Geschke O., Kutter J.P., Diamond D., C02 laser microfabrication of an integrated polymer microfluidic manifold for the determination of phosphorus, Lab On a Chip 2003 3 221-223. 

In conventional chip experiments, fluorescence scanners are used for chip read-out. In the case of laser scanners, HeNe lasers are used as excitation sources and photomultiplier tubes as detectors, whereas CCD-based scanners use white light sources. The optical system can be confocal or non-confocal. Standard biochip experiments are performed using two fluorescent labels as... 

A scanner with two lasers for Cy3 and Cy5 labeling is fairly good enough for most of the microarray experiments. However, multiple lasers are necessary for simultaneous detection of all four nucleotide polymorphisms in chip-based SNPs detection. Besides, an extra third flurophore attached to a sequence that specifically binds to a linker region of the DNA spots could be used for spotting quality control. 

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