White light source

Because on CCD setups excitation for D, S, and A images is usually filter-selected from a single white light source the relative intensity of excitation is approximately fixed. Confocal microscopes use separate laser lines, often from distinct lasers, that can (and for optimal imaging should) be independently adjusted. Thus, on CCD setups y (Eq. (7.6)) is constant for a given set of filters whereas on the confocal, it varies from image to image (also, see Sect. 7.4.2). 

The interferometric measurements with RIfS can be parallelized as demonstrated in Figure 18. In this case, instead of white light interferometry, only a few wavelengths are used to allow parallel detection of all measurement dots. A filter wheel selects one wavelength at a time from the white light source, while the CCD camera monitors the intensity distribution at the transducer for all spots, in this case in a microtiter plate35. 

In conventional chip experiments, fluorescence scanners are used for chip read-out. In the case of laser scanners, HeNe lasers are used as excitation sources and photomultiplier tubes as detectors, whereas CCD-based scanners use white light sources. The optical system can be confocal or non-confocal. Standard biochip experiments are performed using two fluorescent labels as... 

DM = Diohroie Mirror WLS = White Light Source CAM = Camera... 

Photolysis of the telluroester in the presence of thiophenol isolation of benzaldehyde. A solution of the telluroester (Ar = 1-naphthyl) (15 mg, 41.7 jjmol) and thiophenol (17 mg, 154 jjmol) in CDCI3 (0.6 mL) was photolysed with a 250 W white light source at 8°C for 2 h. Preparative TLC on silica gel of the reaction mixture, eluting with ethyl acetate/hexa-nes, 1 9, gave benzaldehyde (3.5 mg, 80%). 

Two instruments considered by Cooke and Kerker (1975) had single-valued response functions, presumably because of broad-band (white) light sources and large angular apertures for both incident and scattered light. 

Fig. 1. Schematic diagram ot high-pressure apparatus tor enzyme activity tests. A, C02 cylinder B, syringe pump C, equilibrium cell D, sapphire windows E, magnetic stirrer F, white light source G, pressure transducer H, ball valve I, micrometering valve J, relief valve.

Nanosecond laser Flash Photolysis experiments were performed with 355 and 532 nm laser pulses from a Brilland-Quantel Nd YAG system (5 ns pulse width) in a front face (VIS) and side face (NIR) geometry using a pulsed 450 W XBO lamp as white light source. Similarly to the femtosecond transient absorption setup, a two beam arrangement was used. However, the pump and probe pulses were generated separately, namely the pump pulse stemming from the Nd YAG laser and the probe from the XBO lamp. A schematic representation of the setup is given below in Fig. 7.3. 0.5 cm quartz cuvettes were used for all measurements. 

Fig. 7.3 Experimental setup for the nanosecond laser Flash Photolysis with a white light continuum. A Brilland-Quantel Nd YAG laser delivers the fundamental pulses (355 and 532 nm). A pulsed XBO lamp is used as white light source. The laser signal is split in order to trigger the digital storage oscilloscope (DSO) utilizing a second photodiode (PD). Two separate detection units in different geometries—photomultiplier (PMT) in front face and a PD in side face—detect the signal in the UV/vis and NIR region, respectively. The monochromator is operated by a standard PC...

When excited with a broadband (white light) source, Au nanoparticles will scatter light at the resonance frequency corresponding to Aspr, often giving rise to a brilliant iridescence. In most cases, the nanoparticle is much smaller than Aspr, such... 

Smoke density is measured based on the attenuation of a light beam by the smoke accumulating in the closed chamber. A white light source is located at the bottom of the enclosure, and a photomultiplier tube is mounted at the top. A modified version of Equation 14.19 is used to calculate the specific optical density from the measured transmittance ... 

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