Second-day Experiments

Students will learn how to measure enzyme kinetics and how to determine protein concentration using Bradford s method (Bradford, 1976). 

1 Kinetics of p-nitrophenyl-fi-D-galactoside hydrolysis with (3 -galactosidase 

Knowing the path length and the molar extinction coefficient, we can calculate the equivalent concentration of PNP observed  

the real concentration of PNP obtained in the linear part is 2.58 x 10 6 x 5.5 

In order to calculate enzyme activity, one should divide PNP concentration obtained per minute by the enzyme concentration in the test tube. In order to determine the enzyme concentration in the test tubes, we will apply the Bradford method (Bradford, 1976). 

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