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ProteinChip technology

The ProteinChip System from Ciphergen Biosystems uses patented SELDI (Surface-Enhanced Laser Desorption/Ionization) ProteinChip technology to rapidly perform the separation, detection, and analysis of proteins at the femtomole level directly from biological samples. ProteinChip Systems use ProteinChip Arrays which contain chemically (cationic, anionic, hydrophobic, hydrophilic, etc.) or biochemically (antibody, receptor, DNA, etc.) treated surfaces for specific interaction with proteins of interest. Selected washes create on-chip, high-resolution protein maps. This protein mass profile, or reten-tate map of the proteins bound to each of the ProteinChip Array surfaces, is quantitatively detected in minutes by the ProteinChip Reader. [Pg.262]

Ernst G, Melle C, Schimmel B, et al. Proteohistography—direct analysis of tissue with high sensitivity and high spatial resolution using ProteinChip technology. /. Histochem. Cytochem. 2006 54 13-17. [Pg.397]

Dayal B, Ertel NH ProteinChip technology a new and facile method for the identification and measurement of high-den-sity lipoproteins apoA-I and apoA-II and their glycosylated products in patients with diabetes and cardiovascular disease. [Pg.179]

He QY, Yip TT, Li M, Chiu JF. Proteomic analyses of arsenic-induced cell transformation with SELDI-TOF ProteinChip technology. J Cell Biochem 2003 88 1-8. [Pg.436]

Key Words Alzheimer s disease A(3 amyloid precursor protein SELDI-TOF MS ProteinChip technology. [Pg.71]

In contrast, ProteinChip technology allows the detailed analysis of A(3 fragments and their modifications (22). Peptides are captured on arrays coated with anti-Ap Ab, washed to remove nonspecifically bound fragments, and finally detected by SELDI-TOF MS. The resolution of the technique allows the discrimination of peptides of similar mass and modified products, e.g., oxidized peptides versus native peptides, which differ by only 16 Da. ProteinChip technology has been used extensively to detect Af> fragments in various samples, including cell culture supernatants, serum, plasma, and cerebrospinal fluid (CSF) (21-23). [Pg.74]

Fig. 2. Capture of A(3 using Ab-coated ProteinChip arrays. Preactivated arrays with exposed active sites are shown in (A). Ab are covalently bound to the array via amine groups (B). Exposed active sites are blocked using Tris-containing buffer (C). Samples are added to the array, incubated for 1 1 h (D), and washed to remove nonspecifically bound proteins (E). EAM is applied to each spot (F), and the array is introduced into the ProteinChip reader where the laser is fired onto the chip surface. Proteins retained on the array surface are subsequently resolved via TOF-MS, which displays the mass-to-charge value and signal intensities of each protein (G, H). (Adapted from ProteinChip technology training course, Bio-Rad Laboratories.)... Fig. 2. Capture of A(3 using Ab-coated ProteinChip arrays. Preactivated arrays with exposed active sites are shown in (A). Ab are covalently bound to the array via amine groups (B). Exposed active sites are blocked using Tris-containing buffer (C). Samples are added to the array, incubated for 1 1 h (D), and washed to remove nonspecifically bound proteins (E). EAM is applied to each spot (F), and the array is introduced into the ProteinChip reader where the laser is fired onto the chip surface. Proteins retained on the array surface are subsequently resolved via TOF-MS, which displays the mass-to-charge value and signal intensities of each protein (G, H). (Adapted from ProteinChip technology training course, Bio-Rad Laboratories.)...
Wiesner, A. (2004) Detection of tumor markers with ProteinChip technology. Curr. Pharm. Biotechnol. 5,45-67. [Pg.85]

The usefulness of this technology has been proven by its wide application and frequent publications in recent years. The main advantages of the SELDl-TOF-MS ProteinChip technology include ... [Pg.42]

V. Seibert, A. Wiesner, Th. Buschmann, J. Meuer, Surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDl TOF-MS) and ProteinChip technology in proteomics research. Pathol. Res. Practice 200, 83 (2004)... [Pg.686]

ProteinChip technology [15] special array surfaces are used to selectively retain entire subsets of proteins directly, and in a single step from biological samples. Thus, in contrast to HPLC-MS, which combines elution chromatography with MS, SELDI-MS combines retention chromatography with MS. [Pg.382]


See other pages where ProteinChip technology is mentioned: [Pg.71]    [Pg.71]    [Pg.73]    [Pg.73]    [Pg.73]    [Pg.227]    [Pg.1334]    [Pg.1338]    [Pg.134]    [Pg.398]   


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Arrays ProteinChip technology

ProteinChip

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