Chimeras

Chilled surface drying ChiUproofing Chilton-Colburn analogy Chimeramycins Chimeras... 

Note that the strategies discussed thus far create hybrids in which the orientation of the DNA insert within the chimera is random. Sometimes it is desirable to insert the DNA in a particular orientation. For example, an experimenter might wish to insert a particular DNA (a gene) in a vector so that its gene product is synthesized. To do this, the DNA must be placed downstream from a promoter. A promoter is a nucleotide sequence lying upstream of a... 

Most enzymes consist of several identical or different subunits. It is known that subunits of similar activity but different origin, which therefore differ in size and amino acid composition and sequence, replace each other in oligomeric enzymes, leading to the formation of enzyme chimeras of catalytic activity 47). The feasibility... 

Schlaad H. and Antonietti M., Block copolymers with amino acid sequences Molecular chimeras of pol3fpeptides and synthetic pol3miers, Eur. Phys. J. E, 10, 17, 2003. 

Zhang X., Ishihara T, Corey D. R. Strand invasion by mixed base PNAs and a PNA-peptide chimera. Nucleic Acids Res. 2000 28 3332-3338. 

Borgatti M., Romanelli A., Saviano M., Pedone C., Lampronti 1., Breda L., Nastruzzi C., Bianchi N., Mischiati C., Gambari R. Resistance of decoy PNA-DNA chimeras to enzymatic degradation in cellular extracts and serum. Oncol. Res. 2003 13 279-287. 

Chimera Molecular modeling http //www.cgl.ucsf.edu/chimera/... 

E I is a kinetic chimera Kj and kt are the constants characterizing the inactivation process kt is the first-order rate constant for inactivation at infinite inhibitor concentration and K, is the counterpart of the Michaelis constant. The k,/K, ratio is an index of the inhibitory potency. The parameters K, and k, are determined by analyzing the data obtained by using the incubation method or the progress curve method. In the incubation method, the pseudo-first-order constants /cobs are determined from the slopes of the semilogarithmic plots of remaining enzyme activity... 

This assay has been thoroughly reviewed [34] and is outlined in Fig. 5. In brief, a cell is transfected with a reporter plasmid consisting of a GAL4 response element upstream from luciferase. NRs are produced as chimeras consisting of the GAIA... 

Datta-Mannan A, Stone MJ. Chemokine-binding specificity of soluble chemokine-receptor analogues identification of interacting elements by chimera complementation. Biochemistry 2004 43 14602-11. 

Dircks, Henry. Scientific studies , or, practical, in contrast with chimerical pursuits, exemplified in two popular lectures I. The life of Edward Somerset, second marquis of Worcester, inventor of the steam engine. II. Chimeras of science astrology, alchemy, squaring the circle, perpetuum mobile, etc. London E. F. N. Spon, 1869. 

Huszar, D Sharpe, A., and Jaenisch, R. (1991a). Migration and proliferation of cultured neural crest cells in W mutant neural crest chimeras. Development 112 131-141. 

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