Chimera studies

Barker, E. L. and Blakely, R. D. (1998) Structural determinants of neurotransmitter transport using cross-species chimeras studies on serotonin transporter. Methods Enzymol 296, 475 198. 

Mutation and chimera studies of IL-8 (CXCL8) have identified the ELR motif in the N-terminal domain of IL-8, MGSA (CXCLl), and NAP-2 (CXCL7) as essential for activation of CXCRl and CXCR2 (13). Similar studies with SDF-1 revealed that Lysl and Pro2... 

CCR3 Receptor Structure. Chimera studies of CCR3 and CCRl receptors show that the N-terminal segments of CCR3 appear to be important for eotaxin binding (77). However, eotaxin remained an effective agonist at this chimeric receptor in either calcium flux or chemotaxis assays. These data are consistent with a multisite model for chemokine-chemo-kine receptor interaction. 

Knockdown ES cells can also be used for chimeric analysis (92). For such experiments, the ES cells can be combined with diploid embryos and their ability to contribute to particular lineages can be investigated. As with aU chimera studies, either the ES cell or the host embryos (or both) must carry a ubiquitous genetic marker just as EGFP or lacZ (84). 

Dircks, Henry. Scientific studies , or, practical, in contrast with chimerical pursuits, exemplified in two popular lectures I. The life of Edward Somerset, second marquis of Worcester, inventor of the steam engine. II. Chimeras of science astrology, alchemy, squaring the circle, perpetuum mobile, etc. London E. F. N. Spon, 1869. 

Stewart PA, Wiley MJ. Developing nervous tissue induces formation of blood-brain barrier characteristics in invading endothelial cells a study using quail-chick transplantation chimeras. Dev Biol 1981 84 183-192. 

A basic property of differentiated neuronal cells is the expression of one classical neurotransmitter and several neuropeptides. In vivo studies with NC cells first demonstrated that the choice of neurotransmitter phenotype could be altered by the environment. In these studies, NC cells were examined by means of chimera transplantation. Two of the final NC cell phenotypes are sympathetic,... 

Mclnerney EM, Weis KE, Sun J, Mosselman S, Katzenellenbogen BS (1998) Transcription activation by the human estrogen receptor subtype beta (ERb) studied with ERa and ERb receptor chimeras. Endocrinology 139 4513... 

Carell has recently presented the study of a flavin amino acid chimera to model riboflavin in DNA photolyases [68]. This amino acid LI (Fig. 20) was synthesized in an enantiopure fashion by building the alloxazine ring onto the epsilon amine of lysine. This coenzyme chimera was applied to the problem of repairing DNA damage caused by UV irradiation. LI was incorporated into an 21-residue peptide, P-1, possessing the sequence of the DNA-binding domain of the helix-loop-helix transcription factor MyoD. 

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