Targeted random chimera production

List of Abbreviations GABA, gamma-aminobutyric acid type A 5HT3, 5-hydroxytryptamine type 3 SDM, site-directed mutagenesis PCR, polymerase chain reaction TRCP, targeted random chimera production SDS, sodium dodecyl sulphate... 

A modification of the random chimera production technique was first described by Boileau et al. (1998). Targeted random chimera production (TRCP) allows the user to delimit the area of recombination by inserting a specific fragment of one of the cDNAs into the dual cDNA construct (O Figure 20-3). For this... 

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