Chimeras, random

Barth, John. Chimera. Random House, New York. 1972. 

Note that the strategies discussed thus far create hybrids in which the orientation of the DNA insert within the chimera is random. Sometimes it is desirable to insert the DNA in a particular orientation. For example, an experimenter might wish to insert a particular DNA (a gene) in a vector so that its gene product is synthesized. To do this, the DNA must be placed downstream from a promoter. A promoter is a nucleotide sequence lying upstream of a... 

List of Abbreviations GABA, gamma-aminobutyric acid type A 5HT3, 5-hydroxytryptamine type 3 SDM, site-directed mutagenesis PCR, polymerase chain reaction TRCP, targeted random chimera production SDS, sodium dodecyl sulphate... 

A modification of the random chimera production technique was first described by Boileau et al. (1998). Targeted random chimera production (TRCP) allows the user to delimit the area of recombination by inserting a specific fragment of one of the cDNAs into the dual cDNA construct (O Figure 20-3). For this... 

After following the procedure for random chimera production, the resulting chimeric molecules will have exchanges delimited by the truncated cDNA in step b)... 

Highly active chimeric fusion proteins were constructed by swapping reductase domains of several P450 BM3 mutants. Subsequently, random mutagenesis at the heme domain of the chimeras was applied to generate chimeric variants that were more active than the parental chime-... 

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