Peptide chimeras

Zhang X., Ishihara T, Corey D. R. Strand invasion by mixed base PNAs and a PNA-peptide chimera. Nucleic Acids Res. 2000 28 3332-3338. 

Peptide chimeras of this type have proven extremely valuable for the examination of an array of recognition events. In conjunction with molecular modeling, peptidomimetic substrates and inhibitors of human thrombin were designed and synthesized to evaluate our proposed structure for the thrombin-bound conformation of fibrinopeptide A (Scheme 2i)J%]... 

The provided methods describe (1) the design and synthesis of peptide chimeras, (2) the analysis of receptor binding affinity, and (3) P-hexoseaminidase secretion from RBL-2H3. These protocols have proven particularly useful for the development of both GPCR ligands (9) and differential secretagogs (13). 

The covalent linkage of peptide monomers is an alternative strategy for the synthesis of peptide chimeras. The formation of cystine via the reduction of paired Cys residues is perhaps the most commonly employed methodology for... 

Synthesis of Linear Branched, and Cyclic Peptide Chimera... 

Linear peptides are the most common example of peptide chimeras. In this strategy two different peptide/protein sequences are tandemly linked. These compounds can be chemically assembled using stepwise (16,17) or convergent solid phase synthetic procedures (18). The length and/or the amino acid composition of desired sequences may dictate different approaches. The other possibility is the attachment of monomeric peptides by the formation of nonpeptide... 

In this chimeric peptide construct the aim was to combine the carrier function and immunstimulatory activity of tuftsin derivatives with an epitope derived from HSV gD to achieve an increased antibody response. Tuftsin is a well-known natural tetrapeptide (TKPR) that has a pronounced effect on the immune system (28,29). Polymerized tuftsin (polytuftsin) is also considered as a carrier molecule that increased antibody levels against attached epitopes in mice (30,31). New, sequential oligopeptides based on repeated tuftsin derivatives (H-[Thr-Lys-Pro-Lys-Gly]n-NH2, where n = 2,4,6,8) were developed in our laboratory to eliminate the drawbacks of tuftsin derivatives produced by polymerization. These new, nontoxic, nonimmunogenic compounds have immunostimulatory activity and a minor chemoattractant effect on monocytes (32). An oligotuftsin derivative was used in this study for the synthesis of a peptide chimera containing an HSV peptide epitope. 

Liu B, Han Y, Ferdous A, Corey DR, Kodadek T. Transcription activation by a PNA-peptide chimera in a mammalian cell extract. Chem Biol. 2003 10 909-916. 

The DAP, which was used by Reymond et al. in the synthesis of peptide dendrimers (see above), was adopted by Wickstrom and coworkers as a single building block for assembly of polyamide peptide-like dendrons on a PNA-peptide chimera presynthesized on a TentaGel Sieber resin (Scheme 15.9). Aminoethoxyethoxyacetate spacers were used to separate the various parts of this hybrid structure (peptide, PNA, and dendron) and HATU couphng... 

The DAP polyamide dendrons conjugated to PNA-peptide chimera and decorated with Gd(III) complexes of D03A ligand were tested as plausible candidates for genetic molecular imaging using MR modality (Scheme 15.32). The Tj relaxivity was increased with the dendron generation. 

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