Germline chimeras

Ma CG, Fan LC, Ganassin R, Bols N, Collodi P (2001) Production of zebrafish germline chimeras from embryo cell cultures. Proc Natl Acad Sci USA 98 2461-2466. 

Once thawed, blastomeres cells can be transplanted into a recipient embryo, leading to the formation of a chimera. The purpose of such transplantation is so injected cells contribute to formation of the germline. Chimera production with cryopreserved cells has been successfully reported in rainbow trout (Takeuchi et al, 2001,2004), together with successful germline transmission, although transmission rates were very low. Germline transmission rates were improved after the recipient cells were weakened by irradiation (Joly et al, 1999). In zebrafish, only two adult chimaeric specimens were obtained from a total of 47 injected embryos... 

ES cellsb Simple Inexpensive None None Need to make germline transmitting chimeras Upon germline transmission only half the ES derived offspring will be heterozygotes ES cells carry tissue culture associated genetic damage Not scalable [Pg.28]

Removal of loxP-Flanked Short Genomic Segment In Situ by Crossing Germline-Transmitter Chimeras with a Stable Cre Transgenic Line... 

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