Chemiluminescence enzyme immunoassay

Examples exist of different types of immunobased technology being interfaced to produce an effective analytical system. In a variety of recent methods, immunoaffinity chromatography has been employed for purification, chemiluminescence enzyme immunoassay has been used for quantification of salbutamol and clenbuterol in tissue and plasma from calves and pigs (122), clenbuterol in cattle hair (123), and monensin in chicken tissues (124). In these methods, quantification at sub-ppb levels has been demonstrated. 

Zhao, L.X. and J.M. Lin. 2005. Development of a micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for rapid- and high-throughput analysis of 17 beta-estradiol in water samples. J. Biotechnol. 118 177-186. 

Zhao, L., J.-M. Lin, Z. Li, et al. 2006. Development of a highly sensitive, second antibody format chemiluminescence enzyme immunoassay for the determination of 17[beta]-estradiol in wastewater. Anal. Chim. Acta 558 290-295. 

Pang et al. reported a successful mass screening for congenital adrenal hyperplasia by measuring 17a-hydroxyprogesterone in dried blood samples on filter paper (P2). They used radioimmunoassay but recently Arakawa et al. developed a chemiluminescence enzyme immunoassay of 17a-hy-droxyprogesterone for this purpose (A6). 

A6. Arakawa, H., Maeda, M., and Tsuji, A., Chemiluminescence enzyme immunoassay of 17a-hydroxyprogesterone using glucose oxidase and bis(2,4,6-trichlorophenyl) oxalate-fluorescent dye system. Chem. Pharm. Bull. 30, 3036-3039 (1982). 

Bronstein, I. Voyta, J.C. Thorpe, G.H.G. Krica, L.J. Edwards, B. Chemiluminescent enzyme-immunoassay of alpha-fetoprotein based on an adamantyl dioxetrance phenyl phosphate substrate. Clin. Chem. 1989, 35, 1441-1446. 

Tsuji A, Maeda M, Arakawa H, Shimizu S, Ikegami T, Sudo Y, et al. Fluorescence and chemiluminescence enzyme immunoassays of 17a-hydroxyprogesterone in dried blood spotted on filter paper. J Steroid Biochem 1987 28 33-40. 

McCormack G> Faix J. Evaluation of chemiluminescent enzyme immunoassay for thyroid binding globulin (TBG) and comparison of T4/TBG ratio with free thyroxin index. Clin Chem 1990 36 1149. 

Sekiya K, Saito Y, Ikegami T, et al. Fully automated analyzer for chemiluminescent enzyme immunoassay. In Stanley PE, Kricka LJ, eds. Bioluminescence and... 

Matsunaga T, Kawasaki M, Yu X, Tsujimura N, Nakamura N. (1996). Chemiluminescence enzyme immunoassay using bacterial magnetic particles. Anal. Chem. 68, 3551-3554. 

Matsunaga T, Sato R., Kamiya S, Tanaka T, Takeyama H. (1999). Chemiluminescence enzyme immunoassay using Protein A-bacterial magnetite complex. J. Magn. Magn. Mater. 268, 932-937. 

To solve the problem, we have developed a highly specific and sensitive assay for C-peptide in serum and plasma using specific monoclonal antibody (MoAb) to the N-terminal of the C-peptide molecule. In this report, we describe the assay performance of C-peptide on the LUMIPULSE system. The system is a fully automated chemiluminescent enzyme immunoassay (CLEIA) system that uses AMPPD as a substrate for alkaline phosphatase and ferrite micro-particles as a solid phase. ... 

Nishizono I, lida S, Suzuki N, Kawada H, Murakami H. eds. Rapid and sensitive chemiluminescent enzyme immunoassay for measuring tumor markers. Clin Chem 1991 37 1639-44. 

Various 1,2-dioxetane derivatives have been synthesized and reported. Among them, AMPPD (Adamantyl Methoxy Phenyl Phosphoryl Dioxetane) is a well known dioxetane bearing a spiroadamantyl group at the 3-position and a phenol phosphate at the 4-position, which is now used for chemiluminescence enzyme immunoassay (CLEIA) using alkaline phosphatase with highly sensitive detection. 

Using the substrate of 1,2-dioxetane derivative in combination with an enhancer, we applied a chemiluminescence enzyme immunoassay (CLEIA) for TSH (Thyroid Stimulating Hormone). 

Figure 3. Calibrator curve of TSH, sensitivity of TSH using chemiluminescence enzyme immunoassay and TSH correlation against TOSOH AIA-1800...

Furthermore, this chemiluminescence enzyme immunoassay of TSH was found to have a good correlation against TOSOH AIA-1800 as shown in Figure 3. 

For the chemiluminescence enzyme immunoassay (CLEIA) application, high light yield is not an essential requirement but low background is essential. 

Figure 3. Calibration graph of the avidin-biotin chemiluminescence enzyme immunoassay...

Dotsikas Y, Loukas YL, Siafaka I. Determination of umbilical cord and maternal plasma concentrations of fentanyl by using novel spectrophotometric and chemiluminescence enzyme immunoassay Anal Chim Acta 2002 459 177-185. 

Fig. 3. Left dose-response curves for HRP. Right calibration curves obtained from a sandwich chemiluminescent enzyme immunoassay for Yersinia, enterocolitica.

SIMULTANEOUS MULTIPLEX BIO- AND CHEMILUMINESCENT ENZYME IMMUNOASSAY FOR PCR PRODUCTS DERIVED FROM GENETICALLY MODIFIED PAPAYA... 

Albrecht, S., Ehle, H., Schollberg, K., Bublitz, R., and Horn, A., Chemiluminescent enzyme immunoassay of human growth hormone based on adamantyl dioxetane phenyl phosphate substrate. In Bioluminescence and Chemiluminescence Current Status (P. E. Stanley and L. J. Kricka, eds.), pp. 115-118. Wiley, Chichester, 1991. 

C12. Champiat, D., Bador, H., and Bonneau, M., Pesticides detection using a chemiluminescence enzyme immunoassay. J. Biolumin. Chemilumin. 2, 191 (abstr.) (1988). 

O Toole, A., Kricka, L. J., Thorpe, G. H. G., and Whitehead, T., Rapid enhanced chemiluminescent enzyme immunoassay for ferritin monitored using instant photographic film. Anal Chim Acta 266, 193-199 (1992). 

Stevens, P., Berdischewsky, M., and Huang, N.-Y., Chemiluminescence enzyme immunoassay for the detection of cytoplasmic protein antigens of Candida Albicans. In Immunoassays Clinical Laboratory Techniques for the 1980s (R. M. Nakamura, W. R. Dito, and E. S. Tucker, III, eds.), pp. 205-212. Liss, New York, 1980. 

Mickova, B., T. Kovalczuk, P. Rauch, M. J. Moreno, A. Abad, A. Montoya, E. Ferri, F. Fini, and S. Girotti. 2004. Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides. Anal. Chim. Acta. 

Magliulo, M., Mirasoli, M., Simoni, P., Lelli, R., Portanti, O., and Roda, A. 2005. Development and validation of an ultrasensitive chemiluminescent enzyme immunoassay for aflatoxin Ml in milk. / Agri. Food Chem. 53 3300-3305. 

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