Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Dried blood spots

Chance DH, Adam BW, Smith SJ, Alexander JR, Hillman SL, Hannon WH (1999) Validation of accuracy-based amino acid reference materials in dried-blood spots by tandem mass spectrometry for newborn screening assays. Clin Chem 45 1269-1277. [Pg.148]

A competitive ELISA assay for Lp(a) was recently described (Y4) in which the microtiter plate was coated with Lp(a) purified from a pool of donors. The method is simple and easy to perform, with satisfactory analytical parameters. A good stability and a reproducible coating of plates with the large Lp(a) lipoprotein is, however, critical in this type of assay. Wang et al. (W6) described an indirect sandwich assay for the measurement of Lp(a) in plasma and in dried blood spots, which can be applied to screening elevated Lp(a) levels in newborns (V3, V4). [Pg.108]

V4. Van Biervliet, J. P., Michiels, G., and Rosseneu, M., Quantification of lipoprotein(a) in dried blood spots and screening for above-normal lipoprotein(a) concentrations in newborns. Clin. Chem. (Winston-Salem. NC) 37, 706-708 (1991). [Pg.132]

Time-resolved approaches for multi-analyte immunoassays have been described recently. Simultaneous determination of LH, follicle stimulating hormone (FSH), hCG, and prolactin (PRL) in a multisite manual strip format has been reported. 88 Four microtiter wells are attached to a plastic strip, two-by-two and back-to-back, such that the wells can be read on a microtiter plate reader. In a quadruple-label format, the simultaneous quantitative determination of four analytes in dried blood spots can be done using europium, samarium, dysprosium, and terbium. 89 In this approach, thyroid-stimulating hormone, 17-a-hydroxyprogesterone, immunoreactive trypsin, and creatine kinase MM (CK-MM) isoenzyme are determined from dried blood samples spotted on filter paper in a microtiter well coated with a mixture of antibodies. Dissociative fluorescence enhancement of the four ions using cofluorescence-based enhancement solutions enables the time-resolved fluorescence of each ion to be measured through four narrow-band interference filters. [Pg.469]

Koal, T., Burhenne, H., Romling, R., Svoboda, M., Resch, K., and Kaever, V., Quantification of antiretroviral drugs in dried blood spot samples by means of liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry 19(21), 2995-3001, 2005. [Pg.97]

Thyroid status can be assessed using dried blood spot specimens for epidemiological surveys. Since TSH levels (as a marker of hypothyroidism) are stable in dried blood spot specimens for months this is the preferred assay for monitoring purposes. TSH monitoring is used in this way in India, China, Zaire, Thailand and Indonesia. [Pg.765]

Al-Dirbashi OY, Rashed MS, Brink HJ, Jakobs C, Filimban N, Al-Ahaidib LY, Jacob M, Al-Sayed MM, Al-Hassnan Z, Faqeih E (2006) Determination of succinylacetone in dried blood spots and liquid urine as a dansylhydrazone by liquid chromatography tandem mass spectrometry. J Chromatogr 831 274-280... [Pg.166]

Severe plasma turbidity due to hyperlipidaemia, as found in lipoprotein lipase deficiency, was shown to result in false-positive newborn screening results when dried blood spots on filter paper are used, but does not usually affect the quantitative colorimetric assay employing plasma samples (reference [21] and our own unpublished experience). [Pg.262]

A calibration curve is recorded for each plate (Fig. 4.1.10). 4-MU standards at absolute concentrations of 0.25 pmol (5 pi), 0.5 pmol (10 pi), 1.0 pmol (20 pi), 2.5 pmol (50 pi) and 5.0 pmol (100 pi) are measured in duplicates. The volume for each well is adjusted with demineralized water to the volume used in the assay. Before measurement, 200 pi stop solution are added and the plate is shaken for 5 min on a plate shaker. Fluorescence is read with an excitation wavelength of 355 nm and an emission wavelength of 460 nm. For leukocyte and dried blood measurements blanks that contain substrate and buffer solution without leukocyte homogenate or dried blood spots are prepared. After incubation and addition of the stop solution, the plate is read immediately for all assays based on leukocytes, while one dried blood spot from an arbitrary sample is added to each blank in case of dried blood assays. Hence, hemoglobin is eluted from blood spots for 30 min and these spots are removed again before measurement. It is crucial to match the age of these specimens to the age of the patient samples. If large variations (several weeks) are evident concerning the age of the samples on the same plate then a separate blank for each patient sample has to be prepared. [Pg.306]

The preparation of a large batch of dried blood spot samples (e.g., ten filter paper cards) from one source requires about 5 ml whole blood. These samples can serve as quality control for at least 150 separate runs. Positive controls from patients may also be prepared in that manner and patients are far more likely to consent to this use of their blood due to the small amount necessary. [Pg.307]

Dried blood spots are prepared from native blood on standardized filter paper (Whatman 903, Whatman). The paper has to be fully soaked with blood and dried at room temperature. Blood has to be applied from one side only, to ensure even distribution throughout the filter paper. Do not use any plastic wrapping before the paper is completely dry. For further details, consult the guidelines from your closest newborn screening facility or metabolic laboratory processing dried blood specimens. [Pg.307]

Dried blood spots (3 mm) are punched from filter paper cards (Whatman 903 paper, Whatman) and distributed in a microtiter plate. Each patient sample is run in duplicates. Subsequently, 100 pi of substrate buffer and 100 pi of citrate buffer are added to each specimen. The plate is shaken for 45 min, sealed with tape, covered with aluminum wrapping and incubated at 37°C for 21 h. The reaction is terminated by the addition of 200 pi stop solution and the fluorescence is read with an excitation wavelength of 365 nm and an emission wavelength of 450 nm. [Pg.318]

Leukocytes from normal controls showed activities ranging from 0.1 to 1.38 nmol/mg protein-min. Dried blood spots from healthy subjects displayed activities between 0.3 and 2.6 nmol/spot-21 h. Patient samples are expected to exhibit barely detectable activities. [Pg.318]

Chamoles NA, Blanco MB, Gaggioli D, Casentini C (2001) Hurler-like Phenotype enzymatic diagnosis in dried blood spots on filter paper. Clin Chem 47 2098-2102... [Pg.322]

Hein LK, Meikle PJ, Dean CJ, Bockmann MR, Auclair D, Hopwood JJ, Brooks DA (2005) Development of an assay for the detection of mucopolysaccharidosis type VI patients using dried blood spots. Clin Chim Acta 353 67-74... [Pg.323]

Lukacs Z, Santavuori P, Keil A, Steinfeld R, Kohlschiitter A (2003) Rapid and simple assay for the determination of tripeptidyl peptidase and palmitoyl protein thioesterase activities in dried blood spots. Clin Chem 49 509-511... [Pg.323]

Wang D, Eadala B, Sadilek M, Chamoles NA, Turecek F, Scott R, Gelb MH (2005) Tandem mass spectrometric analysis of dried blood spots for screening of mucopolysaccharidoses I in newborns. Clin Chem 51 898-900... [Pg.324]

Table 4.4.1 Enzyme and protein deficiencies and the associated sphingolipid storage disorders. D Dried blood spot, F fibroblasts, L leukocytes,... [Pg.352]

Reference range for dried blood spots 40-150 pmol/l-h. [Pg.359]


See other pages where Dried blood spots is mentioned: [Pg.212]    [Pg.133]    [Pg.133]    [Pg.90]    [Pg.192]    [Pg.192]    [Pg.553]    [Pg.99]    [Pg.109]    [Pg.115]    [Pg.58]    [Pg.254]    [Pg.263]    [Pg.305]    [Pg.309]    [Pg.318]    [Pg.320]    [Pg.354]    [Pg.355]    [Pg.356]   
See also in sourсe #XX -- [ Pg.57 , Pg.254 , Pg.672 ]

See also in sourсe #XX -- [ Pg.45 , Pg.258 , Pg.347 , Pg.349 , Pg.357 ]




SEARCH



Dry blood spot

© 2024 chempedia.info