Breast cancer MCF

HSIEH c Y, SANTELL R c, HASLAM s z, HELFERICH w G (1998) Estrogenic effects of genistein on the growth of estrogen receptor-positive human breast cancer (MCF-7) cells in vitro and in vivo. Cancer Res. 58 3883-3838. 

YCUNG H J, DCERGE D R, KIMBERLY F A, ALLRED C D and HELFERICH W G (2002) Dietary genistein negates the inhibitory effect of tamoxifen on growth of estrogen-dependent human breast cancer (MCF-7) cells implanted in athymic mice. Cancer Res 62,2474-77. 

When used in tumor cells, fulvestrant was initially described as a potent, competitive growth inhibitor of ER-positive, human breast cancer MCF-7 cells, whose growth is stimulated by estradiol. The compound was ineffective in tumor cell lines without ER, such as MDA-MB-231. The inhibitory effects were more pronounced with fulvestrant than with tamoxifen in the same cell line (Wakeling et al. 1991). 

Sapino A, Pietribiasi F, Bussolati G, and Marchisio PC [1986] Estrogen- and tamoxifen-induced rearrangement of cytoskeletal and adhesion structures in breast cancer MCF-7 cells. Cancer Res 46 2526-2531... 

A. Bielawska, K. Bielawski, K. Chrzanowski, S. Wolczynski, Prolinase-Activated Prodrug for Cancer Chemotherapy. Cytotoxic Activity of Proline Analogue of Chlorambucil in Breast Cancer MCF-7 Cells , Farmaco 2000, 55, 736-741. 

We have used human leukemias (HL-60 and K562), murine leukemia (L1210) and human breast cancer (MCF-7) for most of our studies. 

Ju, Y.H., Allred, C.D., Allred, K.F., Karko, K.L., Doerge, D.R. and Helferich, W.G. (2001). Physiological concentrations of dietary genistein dose-dependently stimulate growth of estrogen-dependent human breast cancer (MCF-7) tumors implanted in athymic nude mice, J. Nutr., 131, 2957-2962. 

Huang Y, Ray S, Reed JC, et al. Estrogen increases intracellular p26Bcl-2 to p21Bax ratios and inhibits taxol-induced apoptosis of human breast cancer MCF-7 cells. Breast Cancer Res Treat 1997 42( 1 ) 73—81. 

DFBcPh and its dihydrodiol were subjected to metabolism, and the extent of DNA binding in human breast cancer MCF-7 cells was assessed." The extent of DNA binding was then compared with that for BcPh and its dihydrodiol and the potent carcinogen BaP. The 1,4-DFBcPh series 2 (anti) DE-derived DNA adducts were also compared with those arising from intracellular oxidation of the dihydrodiol with subsequent DNA binding. These experiments showed that increased molecular distortion decreased metabolic activation to the terminal metabolites but the DE metabolites formed were the DNA-damaging species. 

Sathyamoorthy N, Wang TT. Differential effects of dietary phyto-oestrogens daidzein and equol on human breast cancer MCF-7 cells. Eur. J. Cancer 33, 2384-2389, 1997. 

Yoon H, Liu RH. 2007. Effect of selected phytochemicals and apple extracts on NF-kappaB activation in human breast cancer MCF-7 cells. J Agric Food Chem 55 3167-3173. 

Bielawska, A., Bielawska, K., Chrzanowski, K., and Wolczynski, S. Prolidase-activated prodrug for cancer chemotherapy cytotoxic activity of praline analogue of chlorambucil in breast cancer MCF-7 cells. II Farmaco 55 736-741, 2000. 

The human ER was first cloned and its structure determined from human breast cancer MCF-7 cells (Green et al., 1986 Greene et al., 1986). ERa consists of 595 amino acids separated into six functional domains (Kumar et al., 1987). Over the past decade, all studies to elucidate the molecular events underlying the mode of ER action as well as antiestrogen-designed therapies have focused on the ERa identified and cloned several years ago (Green et al., 1986 Greene et al, 1986 White et al, 1987). 

When the reporter gene was controlled by an AP-1 instead of an ERE element, E2, DES (diethylstilbestrol), tamoxifen, and raloxifene as well as ICI 164,384 all stimulated transcription with ERa. When ER(3 was transfected instead of ERa, E2 and DES had an inhibitory effect, whereas the three antiestrogens exerted a stimulatory effect (Fig. 6). Most interestingly, E2 could reverse the stimulatory effect of raloxifene mediated by ER(3 and the AP-1 element in a dose-dependent manner. Although these experiments were performed in HeLa cells, it is relevant to mention that in human breast cancer MCF-7 and uterine cancer Ishikawa cells (Paech et al., 1997), ER 3 acting at an AP-1 element led to the same observations, namely stimulation by antiestrogens and inhibi-... 

All. Asoh, K., Watanabe, Y., Mizoguchi, H., Mawatari, M Ono, M., Kohno, K., and Kuwano, M., Induction of manganese superoxide dismutase by tumor necrosis factor in human breast cancer MCF-7 cell line and its TNF-resistant variant. Biochem. Biophys. Res. Common. 162,794-801 (1989). 

Two new cyclic depsipeptides, 1962A (176) and 1962B (177), were isolated from the fermentation broth of an unidentified fungal endophyte obtained from K. candel collected in Hong Kong/ Both 176 and 177 were found to contain one D-amino acid each. In the MTT bioassay, 176 displayed weak activity against human breast cancer MCF-7 cells. 

Nava, V.E., Hobson, J.P., Murthy, S., Milstien, S., and Spiegel, S. Sphingosine kinase type 1 promotes estrogen-dependent tumorigenesis of breast cancer MCF-7 cells. Exp Cell Res, 281, 2002, 115-127. 

Chen, W.F., Huang, M.H., Tzang, C.H., Yang, M., and Wong, M.S., Inhibitory actions of genistein in human breast cancer (MCF-7) cells, Biochim. Biophys. Acta, 1638, 187-196, 2003. 

Studies on some cell lines have shown that in tumor models such as mouse epidermal 1B6 cells and MCF-7, ROS were observed to stimulate cell growth in monolayers. In other cell lines, ROS can also be involved in the pathogenesis of cancer. By promoting cell proliferation in the transformed cancer cell lines MCF-7, HeLa, and Jurkat cells, reduced antioxidant levels were implicated in malignant transformation. Overexpression of manganese superoxide dismutase (MnSOD), a normal cellular antioxidant, enzyme was reported to revert transformation or tumor-promotion response in these and other transformed cell tines, such as human melanoma (UACC-903) cells, human breast cancer (MCF-7) cells, and mouse epidermal JB6 cells. ... 

Guan HT, Xue XH, Wang XJ, Li A, Qin ZY. Effects of siRNA targeted to survivin in suppressing proliferation and inducing apoptosis in breast cancer MCF-7 cells. Zhonghua Zhong Liu Za Zhi 2006 28 326-330. 

Jiang L, Chen RS, Li JC. siRNA-cyclin D1 inhibit cell proliferation in breast cancer MCF-7 cell line. Fen Zi Xi Bao Sheng Wu Xue Bao 2006 39 118-122. 

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