Cell monolayers brain microvessel endothelial

Audus KL, Borchardt RT. Characteristics of the large neutral amino acid transport system of bovine brain microvessel endothelial cell monolayers. J Neurochem 1986 47(2) 484 188. 

Audus KL, Borchardt RT. Bovine brain microvessel endothelial cell monolayers as a model system for the blood-brain barrier. Ann N Y Acad Sci 1987 507 9-18. 

Borges N, Shi F, Azevedo I, et al. Changes in brain microvessel endothelial cell monolayer permeability induced by adrenergic drugs. Eur J Pharmacol 1994 269 (2) 243-248. 

Guillot FL, Audus KL. Angiotensin peptide regulation of fluid-phase endocytosis in brain microvessel endothelial cell monolayers. J Cereb Blood Flow Metab 1990 10 (6) 827-834. 

Raub TJ, Audus KL. Adsorptive endocytosis and membrane recycling by cultured primary bovine brain microvessel endothelial cell monolayers. J Cell Sci 1990 97 (pt 1) 127—138. 

Fontaine M, Elmquist WF, Miller DW. Use of rhodamine 123 to examine the functional activity of P-glycoprotein in primary cultured brain microvessel endothelial cell monolayers. Life Sci 1996 59(18) 1521—1531. 

Modulation of the intercellular junctions by inhibiting the cadherin-cadherin interaction at the adherens junction has also been investigated. Peptides derived from the sequence of the extracellular domain of E-cadherin have been shown to modulate the intercellular junction of bovine brain microvessel endothelial cell (BBMEC) and Madin-Darby canine kidney (MDCK) cell monolayers. These peptides enhance the paracellular penetration of marker molecules such as 14C-mannitol and lower the transepithelial resistance of the monolayers.52 55 The use of these cadherin-derived peptides as adjuvants to enhance paracellular permeability of drugs is still under investigation. 

The study by Gil et al. deseribes quaternary ammonium (5-cyelodextrin nanoparticles as drug delivery earriers for doxorubicin, a hydrophobic anticancer drug, across the BBB (Figure 20.6). The cationic properties of the nanoparticles could be adjusted by ineorporating the amount of quaternary ammonium groups. An approximate hydrodynamic radius of 65-88 nm was achieved. The prepared nanoparticles proved to be very permeable across bovine brain microvessel endothelial cells (BBMVEC) monolayers at 100 pg mL", with permeability coefficients equal to or twice higher than that... 

Thompson SE, Cavitt J, Audus KL, 1994. Leucine-enkephalin effects on paracellular and trans-cellular permeation pathways across brain microvessel endothelial-cell monolayers. / Cardiovasc... 

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