Bottles and pipettes

1) Glassware is removed from the soaking baths and cotton wool plugs removed from pipettes (take care not to splash the caustic chloros). 

2) Place calgon metasilicate (CMS) in a boiler and boil for 20 min. Alternatively bottles may be filled with hot CMS, Decon or 7X and left overnight. 

5) Dry in a hot air oven — if any white streaks are found on the sides of the vessels the rinsing procedure is inadequate. This may be a result of failure to rinse sufficiently or it may indicate that the deionised water supply is faulty. 

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In addition to these precautions the necks of all bottles and pipettes etc., should be flamed . This does not have the function of sterilising the glassware but of raising its temperature above the ambient and thus causing an upflow of air around the bottle or pipette. It prevents airborne contaminants from settling into flasks or onto pipettes. 

Today s high potency drugs, intended for local and systemic treatments, depend on reliable delivery systems. As an alternative to squeeze bottles and pipettes, propellant-driven or mechanical-dispensing systems are often used. Aerosol systems are well known in inhalation therapy. They are ready to use and easy to handle. While new propellants will replace the old ones, the environmental and pharmacological discussions will continue. The switch to new propellants must be supported by sufficient clinical and toxicological data. In particular, compatibility problems must be addressed. Furthermore, attention must be paid to the surfactants not under dispute. 

Add a small volume of siliconizing fluid to clean glassware (spinner flask, bottles and pipettes used for handling microcarriers) and wet all surfaces. 

Much time will be saved if each of the solvents (Water, Ether, 5 per cent. Sodium Hydroxide, 5 per cent. Sodium Bicarbonate and 5 per cent. Hydrochloric Acid) be contained in a 30 or 60 ml. bottle fitted with a cork carrying a calibrated dropper. The concentrated sulphimic acid should be kept in a glass-stoppered bottle and withdrawn with a dropper or pipette as required. 

Droplet generation by air pressure differential across the film, e.g. bursting of liquid film in bubbles, froth across bottle mouths and pipette tips... 

To begin the morphological scoring process on GDll, the embryos with their intact yolk sacs are carefully removed with a pipette from the culture bottle and gently dispersed into a culture dish filled with warmed Tyrode s buffer ( 37°G). 

This involves heating the glassware to 160°C for 90-120 min. It is the preferred method for bottles which do not have screw caps when their orifice should be covered with aluminium foil. It is also used for glass Petri dishes and pipettes. Petri dishes should either be stacked in tins or containers specially designed for them or simply held closed with sterilisation tape. This brown paper adhesive tape has pale strips which turn dark brown under sterilising conditions and is used as an indicator of the effectiveness of the procedure. It is available from the 3 M Company Ltd. (Appendix 3). 

The table shows the nominal tnj tfa/concentrations and volumes of the aqueous KI solutions and of the solutions of I2 in CCI4 to be used. Also given are the nominal concentrations of the thiosulfate solutions to be used for titrating and the sizes of burettes and pipettes to be used. The actual, precise concentrations of the solutions used should be read from the labels on the respective bottles. 

Capacitance cell as shown in Fig. 1 oscillator as described in the text and Fig. 2 frequency counter (range at least 0.5 to 5 MHz) shielded coaxial cables with connectors five 50- or 100-mL volumetric flasks a 5-mL Mohr pipette acetone wash bottle rubber pipette bulb. 

Place 10 ml of urine in each of two 20-ml glass bottles fitted with plastic s ew-caps (McCartney bottles), and to one bottle add 1 to 2ml of M sulphuric acid and to the other add sufficient sodium bicarbonate to saturate the solution. Label the bottles A and B (acids and bases) respectively. Fill each bottle with a mixture of chloroform and isopropyl alcohol (9 1), screw on the caps, shake for 5 minutes, and centiifiige for 5 to 10 minutes. Aspirate the top aqueous phase using a Pasteur pipette connected to a water-operated vacuum pump, filter the chloroform extracts throu phase-separating paper to remove residual water, and collect the filtrates in 10-ml conical test-tubes ... 

Add 10 ml of warm trypsin solution using a 10-ml plastic pipette and pipetter. Gently roll the solution over the cell sheet and pour off the trypsin into the discard bottle. 

Preparation. A solut ion of hydrazoic acid in benzene or chloroform is prepared in a three-necked flask fitted with an efficient stirrer, a dropping funnel, a thermometer, and a gas-exit tube. A paste is prepared from 65 g. (1 mole) of sodium azide and 65 ml. of warm water, 400 ml. of benzene or chloroform is added, the mixture is cooled to 0°, and 0.5 mole of coned, sulfuric acid is added dropwise with control of the temperature to 0-5°. The organic layer Is separated and dried over sodium sulfate. The concentration of the solution of hydrazoic acid is determined by transferring a sample with pipette and pipetter (Fig. H-2) to a glass-stoppered bottle, shaking it with distilled water, and titrating with standard alkali. 

LAB 3 employed INAA and used procedures developed by Filby and Musa [6,13,14]. Oil aliquots were irradiated in a uranium fission reactor, and the flux and energy spectrum of emitted gamma radiation was measured. Mercury concentrations were determined by comparison to standards that were irradiated at the same time as the samples. Oil aliquots were removed from sample bottles by pipette and placed in quartz vials that were then torch-sealed prior to irradiation. 

Using forceps, carefully remove each membrane from its hybridization bottle and transfer it face up to a new container containing 20 ml of lx Blocking Buffer each membrane needs its own container (we use a container that is equivalent to the size of a 200-ml pipette-tip container, 4.5 x 3.5 inches). 

Sterile graduated pipettes of 10, 5, 2 and 1 ml, sterile capped 7.5 x 1.3 cm tubes/ small screw-capped bottles, Pasteur pipettes, an overnight broth culture of the test and control organisms (same as for disc diffusion tests), the required antibiotic in powder form, the required solvent for the antibiotic, sterile distilled water - 500 ml and suitable nutrient broth medium. A suitable rack to hold 22 tubes in two rows, i.e., 11 tubes in each row. 

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