Bean seedlings

Figure Bl.14.8. Time course study of the arrival and accumulation of labelled sucrose in the stem of a castor bean seedling. The labelled tracer was chemically, selectively edited using CYCLCROP (cyclic cross polarization). The first image in the upper left comer was taken before the incubation of the seedlmg with enriched hexoses. The time given in each image represents the time elapsed between tire start of the incubation and the acquisition. The spectmm in the lower right comer of each image shows the total intensity...

Some members of the Liliaceae accumulate free azetidine-2-carboxylic acid in a much higher concentration than that found to be lethal to mung bean seedlings, but it is not incorporated into their proteins. Fowden (43) postulated that these plants either had a proline-incorporating system which was more specific than that found in other species, or some subcellular mechanism operated to prevent the homolog from reaching the sites involved in protein synthesis. Data which supported the first suggestion were subsequently obtained (116). 

Mung bean seedlings at different stages of development were treated with 24% (by weight) KOH to extract non-cellulosic polysaccharides as fully as possible. After neutralisation of the extracts, the polysaccharides were isolated by ethanol precipitation, and... 

Figure 6. Galactan synthase activity in relation to (l->4)-P-D-galactan content in the cell walls of mung bean seedlings...

The pathogenicity of DRB to crop plants has been shown to be host-specific (35) and thus is conceivably linked to root exudation. Alstrom (169) found that the pathogenicity of two isolates of Pseudomonas was determined by the major components of the broth culture in which they were applied to bean seedlings. Both isolates were pathogenic to bean seedlings when the broth contained sucrose and peptone or sucrose and yea.st extract. When the broth contained sucrose alone, one isolate was pathogenic and the other was not. Neither isolate was pathogenic when the broth contained yea.st extract or peptone alone (169). 

A non-invasive measurement of phloem and xylem water flow in castor bean seedlings by nuclear magnetic resonance microimaging), Planta 201, 53-63. 

A similar reaction had previously been known to occur in Saccharomyces fragilis (K8) and in bean seedlings (N3). This is an alternative route to UDPGal, but the activity of the enzyme in newborn liver is very low even in the adult it is only one-sixth that of galactose-l-phosphate uridyl transferase (13), as shown in Table 2. 

Vered Y, Grosskopf I, Palevitch D, Harsat A, Charach G, Weintraub MS, Graff F. (1997). The influence of Vida faba (broad bean) seedlings on urinaiy sodium excretion. Planta Med. 63(3) 237-40. 

Mizutani M, Ohta D, Sato R (1993) Purification and characterization of a cytochrome P450 (rran -cinnamic acid 4-hydroxylase) from etiolated mung bean seedlings. Plant Cell Physiol 34 481-488... 

Table III. Lignin Content in Mung Bean Seedlings...

Conversion of the a-D-glucopyranosyl derivative (94a) into the a-D-galactopyranosyl ester (95a) was demonstrated370 in 1951 as the first example of an enzymic reaction of a sugar nucleotide. The enzyme that catalyzes this reaction, namely, uridine 5 -(a-D-glu-copyranosyl pyrophosphate) 4"-epimerase,371 is common in Nature. Purified preparations have been obtained from yeast,372 373 Escherichia coli 374-376 mung-bean seedlings,377 wheat germ,378 and animal tissues.244,379 380... 

Bean seedlings were grown at 60 to 70 F. until they reached a size at which the primary leaves had almost fully expanded and before the growth of the second internode had exceeded 0.5 inch in length. At that time they were placed at temperatures of 35 , 40 , 50 , 60 , 70 , 80 , and 85 F. Varsol No. 1 was carefully brushed on the upper surface of the primary leaves. After several hours, all the plants were moved back to the original temperature. Ten replications per treatment were used. Statistical analysis of the average fresh weights showed no difference between treatments. 

The inhibition of the synthesis of auxin by D-galactose was also shown to be a contributory factor in the retardation of growth ofA vena coleoptiles.538-539 On the other hand, D-galactose-induced evolution of ethylene is known to retard the growth of mung-bean seedlings.540... 

The results in Table I show that, in the presence of plant material, the standard GA3 is spread over a considerably wider range than is usual. However, after the sample is further purified by the second run, the mobility of GA3 returns to normal. The same pattern of distribution, with zone III as maximum, is manifest in the natural run, although at considerably lower fluorescent intensities. For both fractions, the presence of GA3 is confirmed by the capryl system. A portion of zone III of the natural extract gave positive response proportional to concentration in the pinto bean seedling assay and in the dwarf maize mutants I and V assay. The relative activity on both mutants was approximately equal, as is required for GA3 (26). The correlation of relatively specific biological growth activity with chromatographic and chemical behavior affirms the presence of a GA3-like substance in kudzu vine. 

Aliquot of eluate of this zone gave a positive biogrowth response by dwarf maize hybrid I and V and by pinto bean seedling assay ... 

Growth inhibition activities Epicotyls of bean seedlings were treated and changes in the geotropic reaction were recorded. 

Papaya seeds contain a growth inhibitor, caricacin, which was shown to be N-benzyl-thiono-carbamic acid methyl ester (194). This compound (Table 11) inhibits the elongation of mung bean seedlings. Allicin, an essential ingredient of garlic, completely arrests bacterial growth (195). 

Caricacin (N-Benzyl-Thiono-carbamic acid methyl ester) Papaya seeds Inhibits mung bean seedling elongation 194... 

Wardrop, A. J. Polya, G. M. Properties of soluble auxinbinding protein from dwarf bean seedlings. Plant Sci. Lett.,... 

Plants such as mung bean seedlings can be used as bioindicators for toxic elements such as As [267]. Analysis of tree rings can provide information on short-term variations in pollution sources [268]. Elemental fingerprints have also been used to identify sources of plants, including cannabis [269]. 

The semiconservative replication of DNA at the chromosomal level was shown by J. H. Taylor and coworkers. Using autoradiography and bean seedling root cells in tissue culture, they showed that, after a part of a cycle of duplication with [3H]thymidine (a selective label for DNA), the two chromosomes, descended from an original unlabeled chromosome, were both labeled. Following an additional duplication in the absence of labeled thymidine, the labeled chromosome yielded one labeled and one unlabeled descendant, as predicted by the semiconservative mechanism. 

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