Automated mass spectral system

Association Fran aise de Normalisation French Association for Standardisation (Paris, France) Automated Mass Spectral Deconvolution and Identification System (NIST)... 

DAGAN, S., Comparison of gas chromatography-pulsed flame photometric detection-mass spectrometry, automated mass spectral deconvolution and identification system and gas chromatography-tandem mass spectrometry as tools for trace level detection and identification, J. Chromatogr., A., 2000,868,229-247. 

Extensive technical measures have been implemented to allow the ISP to protect confidential, commercial, or sensible military information during on-site analysis. These measures include in particular the option of blinding the GC/MS operating software and security level filters of AMDIS (Automated Mass Spectral Deconvolution and Identification System), the GC/MS raw data processing software. These measures can be applied separately or combined, offering the ISP the choice of a gradual restriction of the information revealed to the IT (for details see Chapter 2). 

Therefore, the information obtained, through onsite GC/MS analysis about the identity of compounds present in a sample should be restricted to chemicals relevant to the aim of the inspection. This is achieved by operating the instrument in a specially developed blinded mode, which shows neither the chromatogram nor mass spectra during or after the chromatographic run. Additionally, if the analysis is conducted in blinded mode, the only available postprocessing software is a specifically developed on-site version of AMDIS (Automated Mass Spectral Deconvolution and Identification System). This software works only with the OCAD, which contains only compounds relevant to the CWC thus, it reports exclusively the presence of compounds for which spectra are in this database library. 

AMDIS Automated Mass Spectral Deconvolution and Identification System... 

Detection of a target analyte may be tedious with GC-SIM because signal interference may conceal the signal. A method for mass spectrum extraction of GC-MS data has been incorporated into an automated software program developed by the National Institute of Standards, called Automated Mass Spectral Deconvolution and Identification system (AMDIS). The LEGO corporation has also developed a software program used to resolve overlapped signals from GC-TOF-MS. 

Although GC-MS spectral analyses and compound identification can be performed manually, the effort can be tedious, inefficient, and problematic for highly complex chromatograms. The authors laboratory makes productive use of a software package developed by the US Department of Defense and NIST for international treaty verification. The Automated Mass Spectral Deconvolution and Identification System (AMDIS) incorporates several algorithms to distill pure component spectra and associated data from intricate chromatograms, and then uses that information to assess whether the component can be attributed to a known compound in the reference database. 

Meyer MR, Peters FT, Maurer HH (2010) Automated mass spectral deconvolution and identification system for GC-MS screening for drugs, poisons, and metabolites in urine. Clin Chem 56(4) 575-584... 

Zhang, W., Wu, P., and Li, C. (2006) Study of automated mass spectral deconvolution and identification system (AMDIS) in pesticide residue analysis. Rapid Commun. 

GC retention index and mass spectral software/ database AMDIS (Automated Mass Spectrometry Deconvolution and Identification System). The AMDIS is distributed with the OCAD for free to SPs. 

Over the past few years, GCxGC has been utilised in air and aerosol analysis. Particularly, GCxGC in combination with a fast acquisition mass spectrometer, for example, time-of-flight mass spectrometer (ToF MS) with a unit-mass resolution, provides extremely high analytical resolution with mass spectral information. Thus, the GCxGC-ToF MS is an exceptionally powerful tool in the air and aerosol analysis. It should be noted, however, that, as already mentioned in previous chapters, the system produces large amounts of data, and it is difficult to identify compounds from these datasets even with the structural nature and the mass spectral information. Therefore, automated procedures for data handling have been developed for identification purposes. 

Pnor to automated sequencing or mass spectrometry, one may wish to concentrate the peptide in a somewhat smaller tube Transfer the sample to a 1500 or 500 uiL Eppendorf polypropylene tube and combine with a rinse of the 12 x 75 mm tube (rinse with 100 pL of 80% MeCN/0.01% TFA it is advisable to rinse the tube several times if practicable). Immediately remove the solvent by vacuum centrifugation. We advise using only the transparent (uncolored) Eppendorf tubes to handle peptide solutions. Do not use tubes of any kind with O rings or septum closures because they have been shown to contain compounds that can interfere with mass spectral analysis. If a microbore HPLC system is available,... 

NIST have produced a freely available package called AMDIS (automated mass spectrometry deconvolution and identification system) for the analysis of GC/MS data sets. Developed to assist in the task of verifying the international Chemical Weapons Convention (http //www.opcw.org/) financially supported by the US Defense Special Weapons Agency (DSWA, US Department of Defense) the AMDIS program is also distributed with the NIST 02 Mass Spectral Library (see above). 

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