Cytometry assay

Lecoeur, H., Prevost, M.C. and Gougeon, M.L., 2001, Oncosis is associated with exposure of phosphatidylserine residues on the outside layer of the plasma membrane A reconsideration ofthe specificity of the annexin V/propidium iodide assay. Cytometry, 44 65-72. 

Earley MC, Vogt RF Jr, Shapiro HM, et al. Report from a workshop on multianalyte microsphere assays. Cytometry 2002 50 239-42. 

Cavaliere A, BucciareUi E, Sidoni A, Bianchi G, Pietropaoli N, et al. Estrogen and progesterone receptors in breast cancer Comparison between enzyme immunoassay and computer-assisted image analysis of immunocytochemical assay. Cytometry 1996 26 204-08. 

Ordonez, J. V. Wehman, N. M. Amphotericin B susceptibility of Candida species assessed by rapid flow cytometric membrane potential assay. Cytometry 1995, 22, 154-157. 

Buenz, E. J. Limburg, P. J. Howe, C. L. A high-throughput 3-parameter flow cytometry-based cell death assay. Cytometry 2007, 71A, 170-173. 

Azas, N. Di Giorgio, C. Delmas, E Gasquet, M. Timon-David, P. Assessment of amphotericin B susceptibility in Leishmania infantum promastigotes by flow cytometric membrane potential assay. Cytometry 1997,28,165-169. 

We will first summarize the fluorescence and spectroscopic assays that have been developed for the fluorometer and then describe their applications using flow cytometry. We will summarize research which exemplifies the utility of simultaneous measurement of responses and shows how these methods have provided Information about the signal transduction pathways and activation in neutrophils. 

CLARKE R G, LUND E K, JOHNSON I T aud PINDER A c (2000) Apoptosis cau he detected in attached colonic adenocarcinoma HT29 cells using annexin V binding, hut uot hy TUNEL assay or suh-GO DNA content . Cytometry, 39 141-50. 

The CTAD additive mixture has found application in the monitoring of heparin therapy by either the chromogenic substrate assay or the APTT and in the measurement of platelet markers such as P-selectin (CD62) by flow cytometry (108, 109). 

Finally, multiparameter flow cytometry has the potential to improve existing methodologies for the evaluation of immunotoxicological endpoints such as the natural killer cell (NK) assay and the local lymph node assay (LLNA) to evaluate innate immunity and chemical-induced allergic disease. Accepted methodologies typically utilize radio-... 

Manetz, T.S. and Meade, B J., Development of a flow cytometry assay for the identification and differentiation of chemicals with the potential to elicit irritation, IgE-mediated, or T cell-mediated hypersensitivity responses, Toxicol. Sci.. 48, 206, 1999. 

FIGURE 15.8. Mouse local lymph node assay (LLNA) (ICVAM protocol). Modification using flow cytometry instead of radiolabeling is preferable. 

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