Flow cytometry assay

Manetz, T.S. and Meade, B J., Development of a flow cytometry assay for the identification and differentiation of chemicals with the potential to elicit irritation, IgE-mediated, or T cell-mediated hypersensitivity responses, Toxicol. Sci.. 48, 206, 1999. 

Black CB, Duensing TD, Trinkle LS, Dunlay RT. Cell-based screening using high-throughput flow cytometry. Assay Drug Dev. Technol. 2010 Feb 9(l) 13-20. 

In some instances, flow cytometry assays are a superior alternative to conventional procedures for the determination of equilibrium binding constants (Stein et al., 2001). In contrast to assays that employ radiolabelled ligands, which measure population mean values for binding constants, flow cytometry methods can measure those values in individual cells, revealing heterogeneity in receptor expression within a population of cells or membrane vesicles. Furthermore, small samples can be characterized in a short period of time (hours). This approach to receptor-binding analysis may be limited only by the availability of a properly characterized fluorescent ligand. 

The concentration of inhibitor at 50% inhibition of the binding between human sera and pig kidney cells in flow cytometry assay. N/1 = no inhibition. 

Weaver, J. L., and Torous, D. (2000). Flow cytometry assay for counting micronucleated erythrocytes development process. Methods 21, 281-287. 

Osncentrations of all compounds listed are mg/ml in YPD broth. Flow cytometry assays and standard plate counts were performed as described in the footnote to Table 5. 

For simultaneous detection of live and dead cells, the appropriate combination of two (or more with the more advanced flow cytometers) markers can be used in a flow cytometry assay. 

Richardson, M. P Ayliffe, M. J. Helbert, M. Davies, E. G. A simple flow cytometry assay using dihydtor-hodamine for the measurement of the neutrophil respiratory burst in whole blood comparison with the quantitative nitrobluetetrazoUum test. J. Immunol Methods 1998, 219, 187-193. 

Kalgraff, C. A. K. Wergeland, H. I. Pettersen, E. F. Flow cytometry assays of respiratory burst in Atlantic salmon (Salmo salar L.) and in Atlantic cod (Gadus morhua L.) leucocytes. Fish Shellfish Immunol. 2011, 31, 381-388. 

Diaz, T. M. Pertega, S. Ortega, D. Lopez, E. Centeno, A. Manez, R. Domenech, N. FDA/PI flow cytometry assay of complement-mediated cytotoxicity of antibodies generated during xenotransplantation. Cytometry, Part A 2004, 62A, 54-60. 

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