Laser-scanning cytometry assay

Smolewski P, Ruan Q, Vellon L, et al. Micronuclei assay by laser scanning cytometry. Cytometry. 2001 45(l) 19-26. 

Deptala, A., Bedner, E., Gorczyca, W., and Darzynkiewicz, Z. (1998) Activation of nuclear factor kappa B (NF-kB) assayed by laser scanning cytometry. Cytometry 33, 376-382. 

Darzynkiewicz, Z., Smolewski, P., Holden, E., et al., 2011. Laser scanning cytometry for automation of the micronucleus assay. Mutagenesis 26,153—161. 

Special emphasis is placed on the carbohydrate-mediated cell - target system interaction by describing hints and pitfalls of assays for cytoadhesion, specificity, cytoinvasion, and cytoevasion. In addition, basic considerations are presented to discriminate between active and passive uptake as well as to detect lysosomal accumulation. Finally, the pros and cons of two useful analytical techniques, namely, flow cytometry and confocal laser scanning microscopy, are described in detail. 

Bowen WP, Wylie PG. Application of laser-scanning fluores- 96. cence microplate cytometry in high content screening. Assay... 

As with flow cytometry, multiparameter apoptosis assays may also be performed by confocal laser scanning microscopy (CLSM). Using the approach similar to that detailed above for flow cytometry, we have examined NADPH content, mitochondrial membrane potential (CMX Rosamine fluorescence), and mitochondrial mass (Mitotracker Green), by CLSM. Figure 3 shows an example of a typical multiparameter assay performed by confocal microscopy. 

Unlike the conventional flow cytometry which scans the signal of individual bead, the Nanoplex Reader Raman Instrument acquires a total signal from the whole population of the beads within the laser spot (150 pm). Thus, increasing the portion of the beads that carry the specific signal in the laser spot area should improve the limit of detection of the assay. A multiple probe scheme is created to facilitate this characteristic (Fig. 19.5). In the assay, target DNA was first chopped into small... 

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