Antibodies anti-human

Stain slides with 40 pL of titrated secondary antibodies. Anti-human (or other species) IgG, IgM, or IgA antibodies with distinct fluorescent tags, Cy3, Cy5, orFITC, are mixed and then applied to the chips. 

Anti-haemocyanin antibody Anti-(human serum) antibody... 

The greatest selectivity of detection, however, has been achieved by covering piezoelectric sensor surfaces with biological antibodies. Roederer and Bastiaans obtained quantitative sensor response to the presence of human IgG dissolved in both buffer and diluted blood serum (70). The sensor was a quartz SAW device which had the antibody anti-human IgG covalently bonded to its surface. Selectivity was exhibited because response to the protein IgG was obtained in the presence of an excess of other blood serum proteins. In later work the limit of detection of an antibody-bonded SAW device was found to be 2 ng of antigen (82). 

Figure 9.5 Simultaneous quantitation of two proteins using a direct ELISA coupled to the ICP-MS detection. In this experiment, two proteins (human IgG and 3xFLAG-BAP) in IxPBS were incubated in triplicate for 1 h at room temperature to allow binding to the surfaces of the maleic anhydride plate. Negative controls consisted of 100 pf PBS without protein. The plate was probed with primary antibodies anti-human Fab -nanoAu and anti-FLAG-Eu, washed, and acidified with 10% MCI, 1 ppb Ir and 1 ppb Ho. ...

Albumin (Human) Anti- Human-Serum-Albumin (Immuno-Reaction) not reacted antibody 0.1 M NaOH Ag2S 0.5-30 pg/ml... 

A number of chimerized, humanized, and one human mAb have now been approved for therapeutic use in humans in the treatment of autoimmunity, malignancy, infection and cardiovascular disease (Table 1). Some of the currently licensed mAb will be discussed here. A much larger number of mAb are currently being evaluated in Phase I, II and III trials. In general, chimeric, humanized and human mAb are very well tolerated with few side effects. Chimeric or humanized mAb still have the potential to evoke host immune response to the variable domains or CDRs of the antibody so-called HACA (human anti-chimeric antibody) or HAHA (human anti-human antibody) responses, although these responses are uncommon. Short-lived and occasionally severe infusion-related acute hypersensitivity reactions such as fever, skin itching, shivering, respiratory compromise and low blood pressure sometimes occur-. Such effects may... 

Somatostatin. Figure 1 Somatostatin-like im mu noreactivity in neurons of the periventricular hypothalamic nucleus of the rat. Coronal brain cryostat sections have been processed for im mu nohistochemistry and sequentially incubated with a primary monoclonal mouse anti human somatostatin antibody and secondary antimouse antibody conjugated with the fluorescence-dye Cy-3. Images have been taken with a Zeiss Axioplan fluorescence microscope. Scale bar, 100 pM. 

K., Abe, O., and Saito, K. (1987). Antitumor effect of adria-mycin entrapped in liposomes conjugated with anti-human alfa-fetoprotein monoclonal antibody, Cancer Res., 47, 4471-4477. 

Noguchi, A., Takahashi, T., Yamaguchi, T., Kitamura, K., Takakura, Y., Hashida, M., and Sezaki, H. (1992) Preparation and properties of the immunoconjugate composed of anti-human colon cancer monoclonal antibody and mitomycin C—Dextran conjugate. Bioconjugate Chem. 3, 132-137. 

The first hurdle encountered during the development of alfalfa as a recombinant protein production system was the relative inefficiency of the available expression cassettes. A study in which a tomato proteinase inhibitor I transgene was expressed in tobacco and alfalfa under the control of the cauliflower mosaic virus (CaMV) 35S promoter showed that 3-4 times more protein accumulated in tobacco leaves compared to alfalfa leaves [5]. Despite the low efficiency of the CaMV 35S promoter in alfalfa, bio-pharmaceutical production using this system has been reported in the scientific literature. Such reports include expression of the foot and mouth disease virus antigen [6], an enzyme to improve phosphorus utilization [7] and the anti-human IgG C5-1 [8]. In this last work, the C5-1 antibody accumulated to 1% total soluble protein [8]. 

Fig. 8.1 Western blot analysis of transgenic lines showing the expression of an assembled monoclonal antibody in transgenic chloroplasts. Lane 1 Extract from a chloroplast transgenic line, Lane 2 Extract from an untransformed plant. Lane 3 Positive control (human IgA). The gel was run under non-reducing conditions. The antibody was detected with an AP-conjugated goat anti-human kappa antibody.

Human IgG monoclonal antibody C5 IgG Anti-human globulin reagent for phenotyping and crossmatching red blood cells of receivers and donors M. sativa 35 S MSP + 0.13-1.0% TSP 38... 

For other plant-derived antibodies, stability was shown to be similar to mammalian counterparts. For instance, a humanized anti-herpes simplex virus monoclonal antibody (IgGl) was expressed in soybean and showed stability in human semen and cervical mucus over 24 h similar to the antibody obtained from mammalian cell culture. In addition, the plant-derived and mammalian antibodies were tested in a standard neutralization assay with no apparent differences in their ability to neutralize HSV-2. As glycans may play a role in immune exclusion mechanisms in mucus, the diffusion of these monoclonal antibodies in human cerival mucus was tested. No differences were found in terms of the prevention of vaginal HSV-2 transmission in a mouse model, i.e. the plant-derived antibody provided efficient protection against a vaginal inoculum of HSV-2 [58]. This shows that glycosylation differences do not necessarily affect efficacy. 

Comparative evaluation of anti-human thyroid stimulating hormone (hTSH) antibody, bound to the fifth-generation ammonia core (N5) or the fifth-generation ethylenediamine core (E5) dendrimer (1), did not show any differences in either the effective protein concentrations or the shape of the dose-dependent response curves (calibration curves) as determined from the recovery of standard controls. All the other experiments described here were thus carried out with the fifth-generation (i.e. dia. = 70 A) particles of ethylenediamine core (E5) de-ndrimers. The later particles were selected for their ability to be produced reproducibly on a large scale. 

Using a concentration jump as the perturbation, Sutherland et a/.(113) measured the kinetics of binding of fluorescein-labeled human IgG (present as an antigen in solution) to surface-immobilized sheep anti-human IgG. Two TIRF surfaces were used a planar slide and a fiber-optic cylinder. Also using a TIRF recovery after a concentration jump, Kalb et a/,(114) measured the slow ( 10 4 s 1) unbinding kinetics of anti-trinitrophenol (TNP) antibodies in solution and a TNP-derivatized lipid in a planar bilayer. 

It is well known that a very important feature of many biological systems is specific recognition at the molecular level. Antibodies as a group are widely used for molecular recognition, e.g., affinity assays. This feature can be used by labeling an anti-human growth hormone antibody fraction with a fluorescent tag... 

Patients and blood donors are routinely screened for exposure to HIV by means of ElISA and Western blot assays of blood samples (F uie 1-7-15). The assays are designed to detect antibodies to HIV in the blood of the test subject The ELISA is used as the primary screening assay because it is very sensitive. Because the reference interval for the test is set to include everyone with antibodies to HIV, it also gives false positives and thus has a rather low positive predictive value, especially in low-risk populations. The Western blot (or immunoblot) is used as the confirmatory test for HIV exposure. In the Western blot technique, specific HIV proteins are separated by gel electrophoresis and blotted to a filter. The filter is incubated with the test sample. If the sample contains antibodies to HIV, they will bind to the proteins on the filter. The filter is next washed and incubated with a labeled goat anti-human IgG to visualize any bound human antibodies. The Western blot is highly specific. The combination of an ELISA and Western blot has a positive predictive value of greater than 99%,... 

Figure 2.8. Scheme of a chimeric peptide with examples for each of the distinct domains. 0X26, anti-rat transferrin receptor monoclonal antibody (mAh) 84-15, anti-human insulin receptor mAh cHSA, cationized human serum albumin VIP, vasoactive intestinal polypeptide DALDA, dermorphin analogue NGF, nerve growth factor BDNF, brain-derived neurotrophic factor PNA, peptide nucleic acid (3-gal, (3-galactosidase. 

With regard to transport capacity, the introduction of the anti-human insulin receptor antibody (HIR MAb) 83-14 as a vector indicates the potential for future improvements in brain-specific delivery vectors. Compared to anti-TfR monoclonal antibodies, the brain de-hvery in primates is over 7-fold higher due to the high PS product of the HIR MAb. 

Diluted human serum (1 pL) was incubated with the peptide microarray and bound antibodies were detected using a rhodamine-labeled anti-human IgG. Signal was detected using a slide scanner (Affymetrix model 418) with data collection in the Cy3 channel. A reported eightfold gain in sensitivify at 100% specificity over standard ELISA was achieved using the peptide microarray. 

Fig. 2 Determination of the binding stoichiometry of human serum albumin (HSA) to its mouse monoclonal IgG antibody (anti-HSA). (A) The concentration of anti-HSA was 0.33 /J.M. DNS-E was dansylglutamic acid used as internal standard. The intermediate species is considered to be due to the 1 1 complex. (B) A plot of the concentration of free ligand vs. the ratio of [HSA]/[anti-HSA] gives a sharp break at the stoichiometric point. (Reprinted with permission from Ref. 8. Copyright 1994 American Chemical Society.)...

Nitta, T, K. Sato, K. Okumura, and S. Ishii, Induction of cytotoxicity in human T cells coated with anti-glioma x anti-CD3 bispecific antibody against human glioma cells. J Neurosurg, 1990. 72(3) 476-81. 

HAMA Human against mouse antibody (anti-murine antibody) reactions... 

Carter, P, Presta, L., Gorman, C.M., et al. (1992). Humanization of an anti-pl85Her2 antibody for human cancer-therapy. Proc. Natl. Acad. Sci. USA, 89, 4285 1289. 

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