Liposome conjugate with

K., Abe, O., and Saito, K. (1987). Antitumor effect of adria-mycin entrapped in liposomes conjugated with anti-human alfa-fetoprotein monoclonal antibody, Cancer Res., 47, 4471-4477. 

Y. Saeki, N. Matsumoto, Y. Nakano, M. Mori, K. Awai, and Y. Kaneda. Development and characterization of cationic liposomes conjugated with HVJ (Sendai virus) reciprocal effect of cationic lipid for in vitro and in vivo gene transfer, Hum. Gene Ther. 8 2133-2141 (1997). 

Onuma, M. Yasutomi, Y. Yamamoto, M. Watarai, S. Yasuda, T. Kawakami, Y. Antitumor effect of adriamycin entrapped in liposomes conjugated with anti-bovine tumor antigen monoclonal antibody in leukemic cows. Zentralbl. Veterinarmed. 1989, 36, 139-147. 

Mix lipids (as showing on Table 2 for preparing 30 mg of appropriate sample) in a 250 mL round bottom flask according to the desired lipid molar ratio. For liposomal conjugation with an antibody or similar targeting agent, MPB-PE or PEG-PE is to be added. 

These bolamphiphiles bind to and compact DNA, encapsulating in self-assembled iipsomes. Bolamphiphiiic liposomes conjugated with targeting vectors can direct DNA to specific sites. 

Figure 22.22 A protein may be conjugated with a liposome-containing PE groups using a carbodiimide reaction with EDC.

Dissolve the protein or peptide to be conjugated at a concentration of lOmg/ml in 0.5M sodium carbonate, pH 9.5. Mix the activated liposome suspension with the polypeptide solution at the desired molar ratio to effect the conjugation. Mixing the equivalent of 4 mg of protein per mg of total lipid usually results in acceptable conjugates. 

Figure 14.17. Liposome fluoroimmunoassay depiction, in which antigen-liposome conjugates containing fluorescent dye (concentration quenched) compete with analyte antigen for antibody binding sites, followed by wash and detergent lysis, to release the fluorophore for fluorescence measurement.

However, there are problems associated with the use of antibody—liposome conjugates for drug delivery in vivo. Particularly, since lipid vesicles are huge compared to... 

---