Ammonium sulfate test

If unpreso ed ethyl ether is used as a mobile phase constituent, it must be routinely tested for the presence of peroxides. A visual ferrous ammonium sulfate test is conducted as follows [798] SmL 1% ferrous ammonium sulfate (fi eshly prepared), O.SmL IN sulfuric acid, and 0.5mL 0.1 N ammonium thiocyanate ate mixed (and decolorized with a trace of zinc dust if necessary) and shaken with an equal quantity of the ether to be tested. If peroxides are present, a red color develops. This method is significantly more sensitive than the KI test described in Chapter 1. However, inqrroper storage can drastically increase the rate of peroxide formation and present a safety hazard to the user. This is especially true when pre- or postanalysis sanqrle concentration stq>s involve ether (e.g., extractions or preparative LC). 

Qualitative Analysis. Nitric acid may be detected by the classical brown-ring test, the copper-turnings test, the reduction of nitrate to ammonia by active metal or alloy, or the nitrogen precipitation test. Nitrous acid or nitrites interfere with most of these tests, but such interference may be eliminated by acidifying with sulfuric acid, adding ammonium sulfate crystals, and evaporating to alow volume. 

A simple test for ether peroxides is to add lOmL of the ether to a stoppered cylinder containing ImL of freshly prepared 10% solution of potassium iodide containing a drop of starch indicator. No colour should develop during one minute if free from peroxides. Alternatively, a 1% solution of ferrous ammonium sulfate, O.IM in sulfuric acid and O.OIM in potassium thiocyanate should not increase appreciably in red colour when shaken with two volumes of the ether. 

Self-Test H.1B Write the balanced chemical equation for the reaction of solid magnesium nitride with aqueous sulfuric acid to form aqueous magnesium sulfate and aqueous ammonium sulfate. 

A wheat germ, cell-free, translation extract was fractionated into three concentrated parts using ammonium sulfate the 0 - 40 % saturated fraction, the 40 - 60 % saturated fraction, and the ribosome fraction. These fractions were tested for their ability to enhance the translational activity of the wheat germ, cell-free extract for dihydrofolate reductase. The fortified cell-free system supplemented with the 0 - 40 % ammonium sulfate fraction enhanced the efficiency of protein synthesis by 50 %. 

The catalyst was reformulated by Alwin Mittasch, who synthesized some 2500 different catalysts and performed more than 6500 tests. They arrived at a triply promoted catalyst consisting of a fused iron catalyst, with AI2O3 and CaO as structural promoters and potassium as an electronic promoter. The process was first commercialized by BASF, with the first plant located in Oppau in Germany producing 30 tons per day in 1913. The plant initially produced ammonium sulfate fertilizer, but when the First World War broke out it was redesigned to produce nitrates for ammunition. The plant was expanded and in 1915 it produced the equivalent of 230 tons ammonium per day. 

Test T. Dissolve 10 mg of primaquine diphosphate in 5 mL of water and add 1 mL of a 5% solution of ceric ammonium sulfate in dilute nitric acid, a violet color is produced (distinction from chloroquine) [2]. 

Doxil , of which I had the pleasure of being one of the inventors (1-12), serves as an example of successful passive targeting to tumors in animals and humans (1,3,4,13). The ammonium sulfate (AS) transmembrane inside high/outside low gradient-driven loading is also the basis for doxorubicin (DOX) loading for Targeted Doxil (14), which soon will be tested in clinical trials. 

Folin solution analychem An aqueous solution of 500 grams of ammonium sulfate, 5 grams of uranium acetate, and 6 grams of acetic acid in a volume of 1 liter used to test for uric acid. fo-lon S3,lu-sh3n ... 

The solubility of Kelex 100 has been reported to be 4.4 (pH 0.5), 1.4 (pH 1.0), and 1.6 (pH 1.5)ppm, in tests employing 0.5moldm Kelex 100 in Solvesso 150 diluent for the extraction of copper from solutions containing about 30 g dm of metals (Cu, Ni, and Co) [21]. Furthermore, over several months no change in the Kelex concentration was found in pilot plant operations with a 0.5moldm Kelex solution [21]. At higher pH values (up to 9) Kelex solubility has been determined at < 1 ppm in ammonium sulfate solutions up to 300 g dm [22]. It would be expected that the amphoteric nature of Kelex would show increasing solubility at pH values above 7, and lower than about 1, similar to the variation in solubility of... 

The cell-bound amylopullulanase was solubilized with detergent and lipase. It was then purified to homogeneity by treatment with streptomycin sulfate and ammonium sulfate, and by DEAE-Sephacel, octyl-Sepharose and puUulan-Sepharose column chromatography (12). The final enzyme solution was purified 3511-fold over the crude enzyme extract with an overall recovery of 42% and had a specific activity of 481 units/mg protein. The average molecular weight of the enzyme was 136,500 determined by gel filtration on Sephacryl S-200 and SDS-PAGE, and it had an isoelectric point at pH 5.9. It was rich in acidic and hydrophobic amino acids. The purified enzyme was quite thermostable in the absence of substrate even up to 90°C with essentially no loss of activity in 30 min. However, the enzyme lost about 40% of its original activity at 95 C tested for 30 min. The optimum tenq)erature for the action of the purified enzyme on pullulan was 90°C. However, the enzyme activity rapidly decreased on incubation at 95°C to only 38% of the maximal 30 min. The enzyme was stable at pH 3.0-5.0 and was optimally active at pH 5.5. It produced only maltotriose and no panose or isopanose from pullulan. 

Methane chemical ionization spectra of ammonium sulfate and ammonium bisulfate are similar to that of sulfuric acid. The elution profile, however, is quite different, as can be seen from Figure 7. If the salts were deposited on the filter in aerosol form, the shape of the elution profile was somewhat narrower and began at a slightly lower temperature compared to the profile obtained when the salt was deposited in aqueous solution from a syringe. In no case, however, did the elution profile of any of the salts tested overlap the profile for sulfuric acid. 

This paper presents the grade-efficiency curves of a 75 n flat bottom cyclone (RWB 1613) provided by the Amberger Kaolin Werke (AKW). It is tested for the ammonium sulfate-water system for both fines removal and product classification. Its results will be compared with the results for fines removal obtained when using an... 

The mixture is tested for peroxide as follows Prepare an approximately 1% solution of ferrous ammonium sulfate. Transfer 5 ml to each of two test tubes and add 0.5 iriL of 0.5 M sulfuric acid and 0.5 iil. of 0.1 M potassium thiocyanate solution to each tube. Add 5 mL of the methylene chloride solution to one of the test tubes and shake well. The aqueous phase in the methylene chloride tube should not develop a brown red color when examined parallel to the blank. 

An acidic solution of tellurium (IV) or tellurium (VI) is treated with sulfur dioxide and hydrazine hydrochloride. Tellurium precipitated from solution can be estimated by gravimetry. Selenium interferes with this test. A volumetric test involves converting tellurium to tellurous acid and oxidizing the acid with excess ceric sulfate in hot sulfuric acid in the presence of Cr3+ ion as catalyst. The excess ceric sulfate is measured by titration with a standard solution of ferrous ammonium sulfate. 

Results For the St. Louis data, the target transformation analysis results for the fine fraction without July Uth and 5th are given in table 6. The presence of a motor vehicle source, a sulfur source, a soil or flyash source, a titanium source, and a zinc source are indicated. The sulfur, titanium and zinc factors were determined from the simple initial test vectors for those elements. The concentration of sulfur was not related to any other elements and represents a secondary sulfate aerosol resulting from the conversion of primary sulfur oxide emissions. Titanium was found to be associated with sulfur, calcium, iron, and barium. Rheingrover ( jt) identified the source of titanium as a paint-pigment factory located to the south of station 112. The zinc factor, associated with the elements chlorine, potassium, iron and lead, is attributed to refuse incinerator emissions. This factor could also represent particles from zinc and/or lead smelters, though a high chlorine concentration is usually associated with particles from refuse incinerators ( ). The sulfur concentration in the refined sulfate factor is consistent with that of ammonium sulfate. The calculated lead concentration in the motor vehicle factor of ten percent and a lead to bromine ratio of about 0.28 are typical of values reported in the literature (25). The concentration of lead in... 

Scott (80) purified red cell acid phosphatase of homozygous types A and B by using ammonium sulfate and DEAE-cellulose chromatography. The relative activity of these isozyme preparations was the same when tested with a number of substrates. Type B enzyme showed small kinetic... 

Fractionation and Purification of Ex-1 Cellulase Component from Driselase. Driselase powder (50g) was extracted with several aliquots of water and the precipitate formed upon salting out with ammonium sulfate (on a saturation between 20% and 80%) was fractionated on a DEAE-Sephadex A-50 column. Each fraction was tested for -glucosi-dase, xylanase, CMCase, Avicelase activities, and protein content. The elution patterns are shown in Figures 1 and 2. 

A mixture of ammonium sulfate and sodium hydroxide was warmed in a test-tube. The gas was tested with moist red litmus paper. 

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