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Amino acids enzyme methods

Brenner, M., and Neiderwieser, A. (1967). Thin-Layer Chromatography (TLC) of Amino Acids. Enzyme Structure. Methods Enzymol 11 39. [Pg.43]

Monosubshtuted hydantoins are a-amino acids cyclically protected at both the carboxyl- and the a-amino group. They can be easily prepared from an aldehyde and isocyanate or by the Bucherer-Bergs synthesis and similar methods. Indeed, the hydantoin synthesis is also a prachcal method for the preparahon of the racemic amino acid. Enzymes belonging to the dihydro-pyrimidinase family hydrolyze hydantoins to the carbamoyl amino acid. The latter can be hydrolyzed in turn to the amino acid by a second enzyme, a carbamoylase. Both enzymes can discriminate between enantiomers and, if their action is cooperative, either the L- or the D-amino acid can be obtained (Scheme 13.10) [36]. What makes the system of special interest is that the proton in the 5-position of the hydantoin ring (it will become the a-hydrogen in the a-amino acid) is considerably more acidic than conventional protons in amino acid esters or amides and much more acidic than the amino acid itself. Thus, the hydantoin can be often racemized in situ at slightly basic pH where the enzymes are stiU stable and active. If these condihons are met. [Pg.206]

Other endpoints that have been used to estimate extracellular enzyme activity include monitoring the concentrations of substrates and products of oxidation or hydrolysis reactions (e.g., H2O2, a-keto acids, amino acids). These methods are limited however, because there are multiple sources and sinks of these compounds in the environment. [Pg.1421]

However, the range of types of amino acids that can be resolved in this way is much greater than just the natural substrates (i.e. peptides made up of the twenty coded amino acids), because methods to relax the specificity of the enzymes have been found, in some cases by using organic solvents for the reactions. Penicillin acylase from Escherichia coli and an aminoacylase from Streptovercillium olivoreti-... [Pg.126]

Ten days after injection of label, the low-molecular-weight pool contained virtually no label, whereas label remained associated with phosphorylase. Subsequent isolation of phosphorylase from different animals over a range of time periods between 10 and 30 days defined an exponential decay, the rate constant of which was taken as the rate of degradation of phosphorylase. This was subsequently confirmed by independent measurement of the rate of turnover of the enzyme using continuous infusion of labeled amino acids—a method that is independent of reutilization artifacts. The rate of turnover of phosphorylase was the same when measured by either method (Beynon et al., 1986 Cookson and Beynon, 1989). [Pg.138]

Enzymatic methods offer in principle the possibility of a direct enantioselective synthesis of amino acids. Enzymes are often used for separation of racemic mixtures, as examplified in the case of methionine. Although racemic methionine is adequate for the animal feed sector, other applications require the enan-tiomerically pure (L)-form. For the resolution, (L)-acylases from Aspergillus sp. are often used, since they can accept a broad spectrum of substrates, are highly active, and very stable under the production conditions. [62]... [Pg.184]

More recently, the activity of serum peptidases was investigated by capillary electrophoresis with electrochemical detection [60]. Increased peptidase activity in blood is characteristic of a number of disease states. In this application, leu-enkephalin was used as a model substrate. Leu-enkephalin and its metabolites were separated and detected with CEEC following on-capillary copper complex-ation. By incorporating copper in the run buffer, peptides were complexed directly on-capillary [61]. The copper(ll) complexes could then be detected at +700 mV by oxidation to Cu(lll). The method shows good selectivity for peptides over amino acids. This method was used to monitor the metabohsm of leu-enkephalin by enzymes present in a serum sample (Eigure 10). [Pg.477]

In many cases only the racemic mixtures of a-amino acids can be obtained through chemical synthesis. Therefore, optical resolution (42) is indispensable to get the optically active L- or D-forms in the production of expensive or uncommon amino acids. The optical resolution of amino acids can be done in two general ways physical or chemical methods which apply the stereospecific properties of amino acids, and biological or enzymatic methods which are based on the characteristic behavior of amino acids in living cells in the presence of enzymes. [Pg.278]

Enzymatic Method. L-Amino acids can be produced by the enzymatic hydrolysis of chemically synthesized DL-amino acids or derivatives such as esters, hydantoins, carbamates, amides, and acylates (24). The enzyme which hydrolyzes the L-isomer specifically has been found in microbial sources. The resulting L-amino acid is isolated through routine chemical or physical processes. The D-isomer which remains unchanged is racemized chemically or enzymatically and the process is recycled. Conversely, enzymes which act specifically on D-isomers have been found. Thus various D-amino acids have been... [Pg.278]

Other immobilization methods are based on chemical and physical binding to soHd supports, eg, polysaccharides, polymers, glass, and other chemically and physically stable materials, which are usually modified with functional groups such as amine, carboxy, epoxy, phenyl, or alkane to enable covalent coupling to amino acid side chains on the enzyme surface. These supports may be macroporous, with pore diameters in the range 30—300 nm, to facihtate accommodation of enzyme within a support particle. Ionic and nonionic adsorption to macroporous supports is a gentle, simple, and often efficient method. Use of powdered enzyme, or enzyme precipitated on inert supports, may be adequate for use in nonaqueous media. Entrapment in polysaccharide/polymer gels is used for both cells and isolated enzymes. [Pg.291]

The choice of a suitable immobilization method for a given enzyme and appHcation is based on a number of considerations including previous experience, new experiments, enzyme cost and productivity, process demands, chemical and physical stabiHty of the support, approval and safety issues regarding support, and chemicals used. Enzyme characteristics that greatly influence the approach include intra- or extraceUular location size surface properties, eg, charge/pl, lysine content, polarity, and carbohydrate and active site, eg, amino acids or cofactors. The size, charge, and polarity of the substrate should also be considered. [Pg.291]

Biotransformations are carried out by either whole cells (microbial, plant, or animal) or by isolated enzymes. Both methods have advantages and disadvantages. In general, multistep transformations, such as hydroxylations of steroids, or the synthesis of amino acids, riboflavin, vitamins, and alkaloids that require the presence of several enzymes and cofactors are carried out by whole cells. Simple one- or two-step transformations, on the other hand, are usually carried out by isolated enzymes. Compared to fermentations, enzymatic reactions have a number of advantages including simple instmmentation reduced side reactions, easy control, and product isolation. [Pg.331]

Currently, a-amino acids are prepared by several routes such as by the fermentation of glucose, by enzyme action on several substances and by the hydrolysis of proteins. Many methods for synthesising the polymers are known, of which the polymerisation of A -carboxyanhydrides is of particular interest, as it yield-products of high molecular weight (Figure 18.24). [Pg.508]

The field of synthetic enzyme models encompasses attempts to prepare enzymelike functional macromolecules by chemical synthesis [30]. One particularly relevant approach to such enzyme mimics concerns dendrimers, which are treelike synthetic macromolecules with a globular shape similar to a folded protein, and useful in a range of applications including catalysis [31]. Peptide dendrimers, which, like proteins, are composed of amino acids, are particularly well suited as mimics for proteins and enzymes [32]. These dendrimers can be prepared using combinatorial chemistry methods on solid support [33], similar to those used in the context of catalyst and ligand discovery programs in chemistry [34]. Peptide dendrimers used multivalency effects at the dendrimer surface to trigger cooperativity between amino acids, as has been observed in various esterase enzyme models [35]. [Pg.71]

Several dozens of aldolases have been identified so far in nature [23,24], and many of these enzymes are commercially available at a scale sufficient for preparative applications. Enzyme catalysis is more attractive for the synthesis and modification of biologically relevant classes of organic compounds that are typically complex, multifunctional, and water soluble. Typical examples are those structurally related to amino acids [5-10] or carbohydrates [25-28], which are difficult to prepare and to handle by conventional methods of chemical synthesis and mandate the laborious manipulation of protective groups. [Pg.275]

Biochemical methods. In a series of similar compounds, such as amino acids or certain types of steroids, a given enzyme will usually attack only molecules with one kind of configuration. If the enzyme attacks only the l form of eight amino acids, say, then attack on the unknown ninth amino acid will also be on the l form. [Pg.142]

In many cases, the racemization of a substrate required for DKR is difficult As an example, the production of optically pure cc-amino acids, which are used as intermediates for pharmaceuticals, cosmetics, and as chiral synfhons in organic chemistry [31], may be discussed. One of the important methods of the synthesis of amino acids is the hydrolysis of the appropriate hydantoins. Racemic 5-substituted hydantoins 15 are easily available from aldehydes using a commonly known synthetic procedure (Scheme 5.10) [32]. In the next step, they are enantioselectively hydrolyzed by d- or L-specific hydantoinase and the resulting N-carbamoyl amino acids 16 are hydrolyzed to optically pure a-amino acid 17 by other enzymes, namely, L- or D-specific carbamoylase. This process was introduced in the 1970s for the production of L-amino acids 17 [33]. For many substrates, the racemization process is too slow and in order to increase its rate enzymes called racemases are used. In processes the three enzymes, racemase, hydantoinase, and carbamoylase, can be used simultaneously this enables the production of a-amino acids without isolation of intermediates and increases the yield and productivity. Unfortunately, the commercial application of this process is limited because it is based on L-selective hydantoin-hydrolyzing enzymes [34, 35]. For production of D-amino acid the enzymes of opposite stereoselectivity are required. A recent study indicates that the inversion of enantioselectivity of hydantoinase, the key enzyme in the... [Pg.103]

Initial approaches to directed evolution of enzymes rested upon the introduction of random mutations in random sites of the enzyme by the use of the error-prone PCR technique [92] or on the DNA-shuffling method [93]. Extensive research has also been reported in which every amino acid site in an enzyme was systematically subjected to saturation mutagenesis [94]. [Pg.111]


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