Alcohol stains, removal

Uses Manufacture of acetate rayon, acetic anhydride, acetone, acetyl compounds, cellulose acetates, chloroacetic acid, ethyl alcohol, ketene, methyl ethyl ketone, vinyl acetate, plastics and rubbers in tanning laundry sour acidulate and preservative in foods printing calico and dyeing silk solvent for gums, resins, volatile oils and other substances manufacture of nylon and fiber, vitamins, antibiotics and hormones production of insecticides, dyes, photographic chemicals, stain removers latex coagulant textile printing. 

Rinse sections in 50 per cent, alcohol to remove excess of stain. 

To clean a drawing tablet, wipe the rubberized surface with a damp cloth (no detergents ). If there is a tough stain that the damp cloth won t remove, use a cloth dampened with denatured alcohol. After removing the stain, follow with water-dampened cloth to remove any residue. 

CHEMICAL PROPERTIES stable under ordinary conditions of use and storage hazardous polymerization will not occur least reactive of the halogens low volatility at ordinary room temperatures reacts vigorously with reducing materials sodium thiosulfate solution or ammoniated alcohol may remove iodine stains reacts with ammonium hydroxide to form shock-sensitive iodides on drying FP (NA) LFLAJFL (NA) AT (NA) HF (0.0 kJ/mol crystal at 25°C, 62.4 kJ/mol gas at 25°C) HC (NA) Hf (15.52 kJ/mol at 386.8K). 

Cucumber cotyledons were inoculated with purified tobacco mosaic virus (TMV) 20 to 24 hours before vacuum infiltration with different concentrations of crude water extracts of plant leaves (4). After 7 days, inoculated leaves were harvested and stored 24 hours in the dark in a moist chamber to remove excess starch. Starch lesions were counted after clearing with alcohol and staining with an iodine-potassium iodide-lactic acid mixture. The inhibitory effects of various extracts were demonstrated by comparing lesion counts of treated cotyledons to counts on control cotyledons. 

Except for problems with delayed or inadequate fixation as noted above, problems with the trichrome stain are usually related to reagents other than the stain. If crystalline material is apparent after the specimen is stained, the crystals are probably mercuric chloride in the fixative which was not adequately removed because the iodine in the alcohol-iodine solution was too weak or because the slide was in this solution too short a time. If crystals are present after treatment with proper-strength iodine-alcohol, they are present in the specimen, which is thus unsatisfactory, and another specimen should be requested. 

Should the stain be unsatisfactory, the slide can be destained by placing it in xylene to remove the cover slip or immersion oil and then placing it in 50% alcohol for 10 min to hydrate the slide. Destain the slide in 10% acetic acid in water for several hours, and then wash it thoroughly first in water and then in 50 and 70% alcohols. Place the slide in stain for 8 min, and then complete the stain procedures. It is helpful to eliminate or shorten the destain step. 

Remove the slide from fixative bath vial and deposit in 95% ethyl alcohol for at least 10 min prior to staining. 

Nail polish remover Rubbing alcohol Wood and concrete stains... 

Rats are sacrificed on gestation day 20. Fetuses are removed by cesarean section after noting the number of resorptions, implantations, and normal fetuses. The size, weight, and any abnormality of each fetus are noted. Two-thirds of the fetuses are eviscerated and then preserved in absolute alcohol for staining with Alizarin Red S for skeletal assessment. The other one-third of the fetuses is fixed in Allen s modification of Bonin s fluid for slicing with a razor blade (Wilson s Technique) to evaluate visceral anomalies. 

Fitz sat up, shaking his head clear of Victor Padorin and Vladimir Garudin both. He quite fancied a brandy himself alcohol was supposed to be good at removing stubborn stains. Fortunately, there was only Skoglund to be seen when Fitz opened his eyes - his own eyes. The Professor was absorbed in whatever was unfolding on the readout panel just below one of the exhibit cabinets. 

AEC is alcohol soluble and its crisp red color contrasts well against hematoxylin. To avoid removal of the alcohol-soluble product, a non-alcohol based stain like Mayer s hematoxylin should be used. AEC has two reactive sites so that when one is converted it turns red but when both are saturated a green-brown color results. Aquamount causes slow loss of the stain and glycerol mounting is required, rendered permanent by sealing the edges of the cover slip. AEC may be chosen because it may be a lower-risk carcinogen compared to DAB. 

Uses Methyl alcohol is a clear, colorless liquid with a slight alcoholic odor. It is used in the synthesis of formaldehyde, methylamine, ethylene glycol, methacrylates, and as an industrial solvent for a number of products (e.g., inks, resins, adhesives, dyes for straw hats). Methyl alcohol is an important ingredient commonly used to prepare grease and dirt remover. It also is used in the manufacture of photographic films, plastics, celluloid, textile soaps, wood stains, coated fabrics, paper coatings, artificial leather, and other industrial products. 

Once on the slide, wax must be removed completely, in order that the aqueous antibody solution can properly adhere to and penetrate the tissue. This is usually done by heating the slides to about 60 °C to soften the wax, and then reversing the procedure described in A above. The slide is immersed in xylene, 100% alcohol and then diminishing concentrations of alcohol until the final buffer is fully aqueous. Note that 50 slides per 250 mL of xylene is the limit before the xylene is no longer effective, and residual wax begins causing artifacts in the final stained tissue. 

Gram-negative bacteria—Bacteria with a surface stain using crystal violet, or gentian violet treated with iodine that can be removed by flooding with alcohol, acetone, or aniline. 

The unusual features of the lipid constituents of M. tuberculosis were first observed by Aronson. The fat, as the lipids were at first termed, had acid-fast staining properties. Saponification of the fat yielded a quantity of soluble matter and a resistant residue. Aronson was able to prepare an acetate from the latter and identified the substance as an alcohol of very high molecular weight. The same author found that organic solvents removed some of the lipids with ease, these consisting largely of free fatty acids. 

Further investigation by Kresling confirmed earlier observations that saponification of the fat gave an alcohol of high molecular weight. He was unable to detect cholesterol in the tubercle lipids. It was found that ethereal hydrogen chloride would remove the acid-fast staining lipid fraction from the bacillary cell. 

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