Absorbance measurements

Using this concept, Burdett developed a method in 1955 to obtain the concentrations in mono-, di- and polynuclear aromatics in gas oils from the absorbances measured at 197, 220 and 260 nm, with the condition that sulfur content be less than 1%. Knowledge of the average molecular weight enables the calculation of weight per cent from mole per cent. As with all methods based on statistical sampling from a population, this method is applicable only in the region used in the study extrapolation is not advised and usually leads to erroneous results. 

Eind the standard deviations of the slopes in matrix (3-78) for row 2, which refers to absorbances measured at 525 nm. 

When using a spectrophotometer for which the precision of absorbance measurements is limited by the uncertainty of reading %T, the analysis of highly absorbing solutions can lead to an unacceptable level of indeterminate errors. Consider the analysis of a sample for which the molar absorptivity is... 

A fiber that is strained and allowed to recover releases a portion of the work absorbed during straining. The ratio of the work recovered to the total work absorbed, measured by the respective areas under the stress—strain and stress—recovery curves, is designated as resiUence. 

Separate sample blanking requires an additional analytical channel, and is therefore wasteflil of both reagents and hardware. An alternative approach that is used on several automated systems, eg, Du Pont ACA, BM-Hitachi 704, Technicon RA-1000, is that of bichromatic analysis (5) where absorbance measurements are taken at two, rather than one, wavelength. When the spectral curves for the interference material and the chromogen of the species measured differ sufficiently, this can be an effective technique for reducing blank contributions to assay error. Bichromatic analysis is effective for blanks of both the first and second type. 

Aminoisoxazoles can be determined photometrically by reaction with sodium 1,2-naph-thoquinone-4-sulfonate and selective extraction of the resulting dye into CCI4 for absorbance measurements. This class of compound can be determined in the presence of sulfonamides, sulfanilamides, hydroxylamines and other select amines (74MI41610). 

Procedure To an aliquot of the sample solution containing 12.5 - 305 p.g of platinum(IV) were added 5 ml of hydrochloric acid - sodium acetate buffer of pH 2.1, 1 ml of O.IM Cu(II) sulphate solution, and 3.0 ml of 0.5% propericiazine solution. The solution was diluted to 25 ml with distilled water, mixed thoroughly, and the absorbance measured at 520 nm against a reagent blank solution after 10 min. The platinum concentration of the sample solution was determined using a standar d calibration curve. 

Atomic Absorption An analytical method in which the sample is converted into a vapor by passing it through a flame or other energy source and the absorbance at a particular wavelength is measured and compared with that of a reference substance. The absorbance measured is proportional to the concentration ot that substance in the sample. 

The cobalt complex is usually formed in a hot acetate-acetic acid medium. After the formation of the cobalt colour, hydrochloric acid or nitric acid is added to decompose the complexes of most of the other heavy metals present. Iron, copper, cerium(IV), chromium(III and VI), nickel, vanadyl vanadium, and copper interfere when present in appreciable quantities. Excess of the reagent minimises the interference of iron(II) iron(III) can be removed by diethyl ether extraction from a hydrochloric acid solution. Most of the interferences can be eliminated by treatment with potassium bromate, followed by the addition of an alkali fluoride. Cobalt may also be isolated by dithizone extraction from a basic medium after copper has been removed (if necessary) from acidic solution. An alumina column may also be used to adsorb the cobalt nitroso-R-chelate anion in the presence of perchloric acid, the other elements are eluted with warm 1M nitric acid, and finally the cobalt complex with 1M sulphuric acid, and the absorbance measured at 500 nm. 

Discussion. Small quantities of dissolved silicic acid react with a solution of a molybdate in an acid medium to give an intense yellow coloration, due probably to the complex molybdosilicic acid H4[SiMo12O40]. The latter may be employed as a basis for the colorimetric determination of silicate (absorbance measurements at 400 nm). It is usually better to reduce the complex acid to molybdenum blue (the composition is uncertain) a solution of a mixture of l-amino-2-naphthol-4-sulphonic acid and sodium hydrogensulphite solution is a satisfactory reducing agent. 

Thus, the analyte absorption is given by the difference between the absorbance measured at low lamp current and the absorbance at high lamp current. 

If the wavelength of maximum absorption of the analyte (Xmax) is known, it can be monitored and the detector may be considered to be selective for that analyte(s). Since UV absorptions are, however, generally broad, this form of detection is rarely sufficiently selective. If a diode-array instrument is available, more than one wavelength may be monitored and the ratio of absorbances measured. Agreement of the ratio measured from the unknown with that measured in a reference sample provides greater confidence that the analyte of interest is being measured, although it still does not provide absolute certainty. 

The plan, then, was to do the next 20 analyses according to a modified protocol that incorporated a weighing step after every volumetric operation. The evaluation of the absorbance measurements (data files VVV.dat, VWV.dat, and WWW.dat) was carried out according to three schemes for the aforementioned steps 2 to 4, to use either the volumetric or the gravimetric results, or use a combination thereof ... 

UV-absorbance measurement at a given wavelength, either on a photometer, which is known to be sufficiently selective, or on a HPLC... 

Absorbance measurements on mixtures of Sb(V) and Sb(III) in hydrochloric acid media, have led Whitney and Davidson - to propose a dimeric equilibrium... 

Rosseinsky and Nicol have investigated in detail the reaction in highly acidic perchlorate media which leads to the production of Mn ". Using [Mn(II)] in the range 2x 10 to 1 x 10 M with [MnO ] 10 M the rate law, found from absorbance measurements at a wavelength of 525 rn.fi, is... 

The problem has been partially resolved in a later note by Peterson and Duke describing their investigation of the reaction between Sn(II) and the ferricinium ion. Ferricinium perchlorate was prepared by oxidation of ferrocene with AgC104 in aqueous perchloric acid from the nature of the ferricinium structure, Fe(III) is unlikely to complex with more than one chloride ion. The reaction, followed by absorbance measurements on the ferricinium ion at 615 m/i, is first-order in both reactants. The chloride-ion dependence indicates a total of five Cl ions in the activated complex, four of which are deduced to be associated with Sn(ll) as SnCU . 

Eor estimation of the total carotenoid content of a sample, generally the absorbance is measured in petroleum ether at 450 nm and a mean value of 2500 forAj is used. In case a known carotenoid is present in the sample, the absorbance measured at the of that carotenoid and its corresponding Aj can be used for quantifi-... 

Direct measurement of adsorptive stripping voltaimnetric peaks using HMDE 0.60 V and accumulation potential of -0.40V Dilution in phosphate buffer and water, analyzed in Vis region Ion pair formation with octadecyltrimethylammonium bromide at pH 5.6, extraction of ion pair into n-butanol Sample solution mixed with 1 M HCl, ethanol and purification on Sephadex DEAE 25 gel, gel beads are filtered off, packed into 1 nun cell and absorbance measured... 

Step j Wavelength jc, CI2 X2 Br Absorbance Measured ) Estimated ) Innovation... 

Step j Wavelength Ch 2 Brj Absorbance Measured Estimated Innovation... 

Fig. 7.45 Outer two components of the magnetic hyperfine spectrum of a W/W source versus a Ta absorber, measured at zero velocity as a function of a longitudinal magnetic field ( Zeeman drive experiment) (from [185])...

In an unpublished study, Grieger and Hansel have used absorbance measurements to monitor the progress of the reaction forming an enzyme-substrate complex of imidazole (S) and metmyo-globin (E) at high pressures. Formation of the enzyme-substrate complex (ES) may be represented by the following equation... 

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